SOS Response
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General Description
A set of reversible checkpoints induced upon DNA damage, or regions of single stranded DNA.
Components
Function
In response to DNA damage, the function of the SOS Response is to postpone Septation and repair the damaged DNA. Many genes involved in DNA repair are expressed upon LexA repressor cleavage.
Regulation
RecA bound to single-stranded DNA leads to its activation. This activated form of RecA, or RecA* causes autocleavage of the LexA repressor[1]. It was later discovered that the mechanism of LexA cleavage is autodigestion catalyzed by RecA*[2].
Cell Biology
The expression of multiple genes is upregulated upon induction of the SOS response. The LexA repressor control the expression of:
- sulA[3]
- ftsK[4]
- uvrA[5]
- uvrB[6]
- Peterson et al. (1988) finds that the derepression of other specific genes promote DNA repair and mutagenesis[7].
Experimental Resources
Effect of antibiotics on the SOS response
Several antibiotics induce the SOS response
- mitomycin C (working concentration-0.1 µg/ml)[4]
- ciprofloxacin (300 ug on filter disc)[8]
- mitomycin C (10 ug on filter disc)[8]
- trimethoprim (10 ug on filter disc)[8]
Comparison with other organisms
Notes
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References
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- ↑ Little, JW () Control of the SOS regulatory system by the level of RecA protease. Biochimie 64 585-9 PubMed
- ↑ Little, JW (1984) Autodigestion of lexA and phage lambda repressors. Proc. Natl. Acad. Sci. U.S.A. 81 1375-9 PubMed
- ↑ Mizusawa, S et al. (1983) Transcription of the sulA gene and repression by LexA. J. Mol. Biol. 171 337-43 PubMed
- ↑ 4.0 4.1 Wang, L & Lutkenhaus, J (1998) FtsK is an essential cell division protein that is localized to the septum and induced as part of the SOS response. Mol. Microbiol. 29 731-40 PubMed
- ↑ Sancar, A et al. (1982) LexA protein inhibits transcription of the E. coli uvrA gene in vitro. Nature 298 96-8 PubMed
- ↑ Sancar, GB et al. (1982) The uvrB gene of Escherichia coli has both lexA-repressed and lexA-independent promoters. Cell 28 523-30 PubMed
- ↑ Peterson, KR et al. (1988) Derepression of specific genes promotes DNA repair and mutagenesis in Escherichia coli. J. Bacteriol. 170 1-4 PubMed
- ↑ 8.0 8.1 8.2 Thi, TD et al. (2011) Effect of recA inactivation on mutagenesis of Escherichia coli exposed to sublethal concentrations of antimicrobials. J. Antimicrob. Chemother. 66 531-8 PubMed
