glpX:Gene

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Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

glpX

Mnemonic
Synonyms

ECK3917, b3925, JW3896[1]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

88.64 minutes 

MG1655: 4113602..4112592
gbrowse_img.php?coord=4112592..4113602&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 4095061..4094051
gbrowse_img.php?coord=4094051..4095061&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 3521102..3522112
gbrowse_img.php?coord=3521102..3522112&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 4213299..4212289
gbrowse_img.php?coord=4212289..4213299&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature type Location Description


Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔglpX (Keio:JW3896)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC10811[3]

glpX221::SpcR

PMID:10986273[4]

CGSC:84041

ΔglpX784::kan

PMID:16738554[2]

CGSC:101194



Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3896

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCAGACGAGAACTTGCCATCGA

Primer 2:CCGAGGATGTGCACCTGCATTTC

zih-35::Tn10

Linked marker

CAG18495 = CGSC7467[3]

est. P1 cotransduction: 11% [6]

pflD501::Tn10

Linked marker

CAG18477 = CGSC7470[3]

est. P1 cotransduction: 30% [6]
Synonyms:zij-501::Tn10 nnnCAG18477 also carries metF79(Am) (CGSC).


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11517

EchoBASE (EcoliWiki Page)

EchoBASE:EB1479

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3925

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C4877

EcoGene (EcoliWiki Page)

EcoGene:EG11517

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:GLPX

CGSC (EcoliWiki Page)

CGSC:30884

ASAP (EcoliWikiPage)

ASAP:ABE-0012821



Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ Donahue, JL et al. (2000) Purification and characterization of glpX-encoded fructose 1, 6-bisphosphatase, a new enzyme of the glycerol 3-phosphate regulon of Escherichia coli. J. Bacteriol. 182 5624-7 PubMed
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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