PMID:10074094: Difference between revisions
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Revision as of 03:42, 9 January 2010
Heath, RJ and Rock, CO (1999) A missense mutation accounts for the defect in the glycerol-3-phosphate acyltransferase expressed in the plsB26 mutant.J. Bacteriol. 181:1944-6
| Abstract | The sn-glycerol-3-phosphate acyltransferase (plsB) catalyzes the first step in membrane phospholipid formation. A conditional Escherichia coli mutant (plsB26) has a single missense mutation (G1045A) predicting the expression of an acyltransferase with an Ala349Thr substitution. The PlsB26 protein had a significantly reduced glycerol-3-phosphate acyltransferase specific activity coupled with an elevated Km for glycerol-3-phosphate. |
| Links | PubMed |
Significance
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Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0005887 |
integral to plasma membrane |
IDA: Inferred from Direct Assay |
C |
The authors detected FLAG-tagged PlmB (expressed from a plasmid) in the membrane preparations of cells (Figure 1A). |
complete | |||
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