pheM:Gene: Difference between revisions

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Revision as of 23:48, 12 December 2009

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

pheM

Mnemonic
Synonyms

ECK1713, b1715, JW1705, phtL, G266[1][2]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

38.74 minutes 

MG1655: 1797294..1797250
gbrowse_img.php?coord=1797250..1797294&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 1800984..1800940
gbrowse_img.php?coord=1800940..1800984&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 1887865..1887821
gbrowse_img.php?coord=1887821..1887865&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature type Location Description
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔpheM (Keio:JW1705)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC9442[4]

ΔpheM787::kan

PMID:16738554[3]

CGSC:102322


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1705

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCAATGCTGCTATTTTCCGCTT

Primer 2:CCGGTGCTAAAGTAAAAAAAGAA

zdi-925::Tn10

Linked marker

CAG12151 = CGSC7385[4]

est. P1 cotransduction: 48% [6]
Synonyms:zdh-925::Tn10

zdj-276::Tn10

Linked marker

CAG18464 = CGSC7387[4]

est. P1 cotransduction: 24% [6]
Synonyms:zdi-276::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11272

EchoBASE (EcoliWiki Page)

EchoBASE:EB1251

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1715

EcoGene (EcoliWiki Page)

EcoGene:EG11272

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:PHEM

ASAP (EcoliWikiPage)

ASAP:ABE-0005727


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Notes

Links

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Name URL Comments
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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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