mtn:Gene Product(s): Difference between revisions

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<span id="Structure"></span><big>'''Structure'''</big>
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<span id='Structure'></span><big>'''Structure'''</big><br />
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==== Structure figures ====
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
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===Notes===
===Notes===


Revision as of 16:23, 23 July 2009

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

Pfs

Synonyms

5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase[1], B0159[2][1], Mtn[2][1], YadA[2][1], Pfs[2][1]

Product description

5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase[2][3];

Component of 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase[3]

5'-Methylthioadenosine nucleosidase; S-adenosylhomocysteine and 5'-deoxyadenosine are also substrates[4]

EC number (for enzymes) 
3.2.2.9[1]
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference(s) Evidence Code with/from Aspect Notes Status
GO:0005737

cytoplasm

PMID:16858726[5]

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc [6]

complete

GO:0003824

catalytic activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000845

F

Seeded from EcoCyc [6]

complete

GO:0008782

adenosylhomocysteine nucleosidase activity

GO_REF:0000002
GO_REF:0000003

IEA: Inferred from Electronic Annotation

InterPro:IPR010049
EC:3.2.2.9

F

Seeded from EcoCyc [6]

complete

GO:0008930

methylthioadenosine nucleosidase activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010049

F

Seeded from EcoCyc [6]

complete

GO:0009116

nucleoside metabolic process

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000845

P

Seeded from EcoCyc [6]

complete

GO:0009164

nucleoside catabolic process

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010049

P

Seeded from EcoCyc [6]

complete

GO:0016787

hydrolase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0378

F

Seeded from EcoCyc [6]

complete

GO:0019509

methionine salvage

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010049

P

Seeded from EcoCyc [6]

complete

GO:0003001

generation of a signal involved in cell-cell signaling

PMID:11489131[7]

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc

complete

GO:0003824

catalytic activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000845

F

Seeded from EcoCyc

complete

GO:0005737

cytoplasm

PMID:16858726[5]

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc

complete

GO:0008782

adenosylhomocysteine nucleosidase activity

GO_REF:0000002
GO_REF:0000003
PMID:9524204[8]

IDA: Inferred from Direct Assay

InterPro:IPR010049
EC:3.2.2.9

F

Seeded from EcoCyc

complete

GO:0008930

methylthioadenosine nucleosidase activity

GO_REF:0000002
PMID:9524204[8]

IDA: Inferred from Direct Assay

InterPro:IPR010049

F

Seeded from EcoCyc

complete

GO:0009116

nucleoside metabolic process

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000845

P

Seeded from EcoCyc

complete

GO:0009164

nucleoside catabolic process

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010049

P

Seeded from EcoCyc

complete

GO:0016787

hydrolase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0378

F

Seeded from EcoCyc

complete

GO:0019509

methionine salvage

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010049

P

Seeded from EcoCyc

complete

GO:0015949

nucleobase, nucleoside and nucleotide interconversion

P

Seeded from EcoCyc 11.1[3].

required fields missing

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase

could be indirect

Protein

Subunits of 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase

could be indirect


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Notes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MKIGIIGAME EEVTLLRDKI ENRQTISLGG CEIYTGQLNG TEVALLKSGI GKVAAALGAT
LLLEHCKPDV IINTGSAGGL APTLKVGDIV VSDEARYHDA DVTAFGYEYG QLPGCPAGFK
ADDKLIAAAE ACIAELNLNA VRGLIVSGDA FINGSVGLAK IRHNFPQAIA VEMEATAIAH
VCHNFNVPFV VVRAISDVAD QQSHLSFDEF LAVAAKQSSL MVESLVQKLA HG

at EcoCyc 

MKIGIIGAME EEVTLLRDKI ENRQTISLGG CEIYTGQLNG TEVALLKSGI GKVAAALGAT
LLLEHCKPDV IINTGSAGGL APTLKVGDIV VSDEARYHDA DVTAFGYEYG QLPGCPAGFK
ADDKLIAAAE ACIAELNLNA VRGLIVSGDA FINGSVGLAK IRHNFPQAIA VEMEATAIAH
VCHNFNVPFV VVRAISDVAD QQSHLSFDEF LAVAAKQSSL MVESLVQKLA HG
Length

232

232

Mol. Wt

24.353 kDa

24.353 kDa

pI

4.9 (calculated)

4.9 (calculated)

Extinction coefficient

5,960 - 6,585 (calc based on 4 Y, W, and 5 C residues)

5,960 - 6,585 (calc based on 4 Y, W, and 5 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure>mtn 562</beststructure>

Models

View models at:

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Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

PDB (EcoliWiki Page)

PDB:1Z5P

EcoCyc (EcoliWiki Page)

EcoCyc:EG11090-MONOMER

SwissModel (EcoliWiki Page)

SwissModel:P0AF12

UniProt (EcoliWiki Page)

UniProt:P0AF12

PFAM (EcoliWiki Page)

PFAM:PF01048

RefSeq (EcoliWiki Page)

RefSeq:NP_414701

ModBase (EcoliWiki Page)

ModBase:P0AF12


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Notes

Links

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Name URL Comments
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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 Lasserre, JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27 3306-21 PubMed
  6. ↑ 6.0 6.1 6.2 6.3 6.4 6.5 6.6 6.7 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  7. ↑ Schauder, S et al. (2001) The LuxS family of bacterial autoinducers: biosynthesis of a novel quorum-sensing signal molecule. Mol. Microbiol. 41 463-76 PubMed
  8. ↑ 8.0 8.1 Cornell, KA & Riscoe, MK (1998) Cloning and expression of Escherichia coli 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase: identification of the pfs gene product. Biochim. Biophys. Acta 1396 8-14 PubMed

Categories

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