copA:Gene Product(s): Difference between revisions
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<span id='Structure'></span><big>'''Structure'''</big><br /> | |||
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==== Structure figures ==== | |||
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===Notes=== | ===Notes=== | ||
Revision as of 15:32, 23 July 2009
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
CopA |
|---|---|
| Synonyms | |
| Product description |
YbaR[2]; Cu+-exporting ATPase[3] Copper-, silver-translocating P-type ATPase efflux pump; involved in copper resistance; copper- and silver-inducible; regulated by copper-responsive CueR activator[4] |
| EC number (for enzymes) | |
| edit table |
Notes
Function
Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference(s) | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0005507 |
copper ion binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc |
complete | |||
| GO:0004008 |
copper-exporting ATPase activity |
IEA: Inferred from Electronic Annotation |
InterPro:IPR006403 |
F |
Seeded from EcoCyc 12.5 [5] |
complete | ||
| GO:0005375 |
copper ion transmembrane transporter activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc 12.5 [5] |
complete | |||
| GO:0003824 |
catalytic activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc [6] |
complete | |||
| GO:0000287 |
magnesium ion binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc [6] |
complete | |||
| GO:0000166 |
nucleotide binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc [6] |
complete | |||
| GO:0016020 |
membrane |
GO_REF:0000002 |
IEA: Inferred from Electronic Annotation |
InterPro:IPR000695 |
C |
Seeded from EcoCyc [6] |
complete | |
| GO:0015992 |
proton transport |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc 12.5 [5] |
complete | |||
| GO:0005507 |
copper ion binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc [6] |
complete | |||
| GO:0005524 |
ATP binding |
GO_REF:0000002 |
IEA: Inferred from Electronic Annotation |
InterPro:IPR000695 |
F |
Seeded from EcoCyc [6] |
complete | |
| GO:0006810 |
transport |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc 12.5 [5] |
complete | |||
| GO:0006811 |
ion transport |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc [6] |
complete | |||
| GO:0006825 |
copper ion transport |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc 12.5 [5] |
complete | |||
| GO:0008152 |
metabolic process |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc [6] |
complete | |||
| GO:0015662 |
ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc 12.5 [5] |
complete | |||
| GO:0016021 |
integral to membrane |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc [6] |
complete | |||
| GO:0016787 |
hydrolase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc [6] |
complete | |||
| GO:0046873 |
metal ion transmembrane transporter activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc 12.5 [5] |
complete | |||
| GO:0016820 |
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc 12.5 [5] |
complete | |||
| GO:0030001 |
metal ion transport |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc 12.5 [5] |
complete | |||
| GO:0046872 |
metal ion binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc 12.5 [5] |
complete | |||
| GO:0031224 |
intrinsic to membrane |
IDA: Inferred from Direct Assay |
C |
Seeded from Riley et al 2006 [1]. CopA was purified from everted membrane vesicles (Fig. 6). |
complete | |||
| GO:0005886 |
plasma membrane |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-1003 |
C |
Seeded from EcoCyc [6] |
complete | ||
| GO:0006754 |
ATP biosynthetic process |
GO_REF:0000002 |
IEA: Inferred from Electronic Annotation |
InterPro:IPR000695 |
P |
Seeded from EcoCyc [6] |
complete | |
| GO:0000166 |
nucleotide binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc |
complete | |||
| GO:0000287 |
magnesium ion binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc |
complete | |||
| GO:0003824 |
catalytic activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc |
complete | |||
| GO:0004008 |
copper-exporting ATPase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc |
complete | |||
| GO:0005524 |
ATP binding |
GO_REF:0000002 |
IEA: Inferred from Electronic Annotation |
InterPro:IPR000695 |
F |
Seeded from EcoCyc |
complete | |
| GO:0005886 |
plasma membrane |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-1003 |
C |
Seeded from EcoCyc |
complete | ||
| GO:0006754 |
ATP biosynthetic process |
GO_REF:0000002 |
IEA: Inferred from Electronic Annotation |
InterPro:IPR000695 |
P |
Seeded from EcoCyc |
complete | |
| GO:0006810 |
transport |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc |
complete | |||
| GO:0006811 |
ion transport |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc |
complete | |||
| GO:0006825 |
copper ion transport |
IMP: Inferred from Mutant Phenotype |
P |
Seeded from EcoCyc |
complete | |||
| GO:0008152 |
metabolic process |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc |
complete | |||
| GO:0010273 |
detoxification of copper ion |
IMP: Inferred from Mutant Phenotype |
P |
Seeded from EcoCyc |
complete | |||
| GO:0015662 |
ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism |
GO_REF:0000002 |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc |
complete | ||
| GO:0016020 |
membrane |
GO_REF:0000002 |
IEA: Inferred from Electronic Annotation |
InterPro:IPR000695 |
C |
Seeded from EcoCyc |
complete | |
| GO:0016021 |
integral to membrane |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc |
complete | |||
| GO:0016787 |
hydrolase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc |
complete | |||
| GO:0016820 |
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc |
complete | |||
| GO:0030001 |
metal ion transport |
IEA: Inferred from Electronic Annotation |
InterPro:IPR006121 |
P |
Seeded from EcoCyc |
complete | ||
| GO:0031224 |
intrinsic to membrane |
IDA: Inferred from Direct Assay |
C |
Seeded from EcoCyc |
complete | |||
| GO:0046872 |
metal ion binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc |
complete | |||
| GO:0015662 |
ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism |
IDA: Inferred from Direct Assay |
F |
Figure 2 |
complete | |||
| GO:0015662 |
ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0006825 |
copper ion transport |
IMP: Inferred from Mutant Phenotype |
P |
Everted membrane vesicles prepared from a ΔcopA strain that expressed copA from a plasmid accumulated 64Cu. Accumulation required ATP. |
complete | |||
| GO:0043682 |
copper-transporting ATPase activity |
F |
required field missing | |||||
| GO:0016021 |
integral to membrane |
ISM: Inferred from Sequence Model |
C |
Predicted transmembrane domains shown below in the table of Domains/Motifs/Modification sites. |
complete | |||
| GO:0010273 |
detoxification of copper ion |
IMP: Inferred from Mutant Phenotype |
P |
copA mutants are sensitive to copper ions. |
complete | |||
| GO:0016887 |
ATPase activity |
IDA: Inferred from Direct Assay |
F |
Fig. 6 shows ATPase activity of CopA purified from membrane vesicles. |
complete | |||
| edit table |
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
|
Protein |
sdhA |
Experiment(s):EBI-1136866 | ||
|
Protein |
glpD |
Experiment(s):EBI-1136866 | ||
|
Protein |
ydcD |
Experiment(s):EBI-1136866 | ||
|
Protein |
dnaK |
Experiment(s):EBI-1136866 | ||
|
Protein |
groL |
Experiment(s):EBI-1136866 | ||
|
Protein |
hflB |
Experiment(s):EBI-1136866 | ||
| edit table |
Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Notes |
|---|---|---|---|
|
plasma membrane |
C-terminus localized in the cytoplasm with 8 predicted transmembrane domains |
Daley et al. (2005) [12] |
|
| edit table |
Notes
Structure and Physical Properties
Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Sequence |
MSQTIDLTLD GLSCGHCVKR VKESLEQRPD VEQADVSITE AHVTGTASAE QLIETIKQAG YDASVSHPKA KPLAESSIPS EALTAVSEAL PAATADDDDS QQLLLSGMSC ASCVTRVQNA LQSVPGVTQA RVNLAERTAL VMGSASPQDL VQAVEKAGYG AEAIEDDAKR RERQQETAVA TMKRFRWQAI VALAVGIPVM VWGMIGDNMM VTADNRSLWL VIGLITLAVM VFAGGHFYRS AWKSLLNGAA TMDTLVALGT GVAWLYSMSV NLWPQWFPME ARHLYYEASA MIIGLINLGH MLEARARQRS SKALEKLLDL TPPTARLVTD EGEKSVPLAE VQPGMLLRLT TGDRVPVDGE ITQGEAWLDE AMLTGEPIPQ QKGEGDSVHA GTVVQDGSVL FRASAVGSHT TLSRIIRMVR QAQSSKPEIG QLADKISAVF VPVVVVIALV SAAIWYFFGP APQIVYTLVI ATTVLIIACP CALGLATPMS IISGVGRAAE FGVLVRDADA LQRASTLDTV VFDKTGTLTE GKPQVVAVKT FADVDEAQAL RLAAALEQGS SHPLARAILD KAGDMQLPQV NGFRTLRGLG VSGEAEGHAL LLGNQALLNE QQVGTKAIEA EITAQASQGA TPVLLAVDGK AVALLAVRDP LRSDSVAALQ RLHKAGYRLV MLTGDNPTTA NAIAKEAGID EVIAGVLPDG KAEAIKHLQS EGRQVAMVGD GINDAPALAQ ADVGIAMGGG SDVAIETAAI TLMRHSLMGV ADALAISRAT LHNMKQNLLG AFIYNSIGIP VAAGILWPFT GTLLNPVVAG AAMALSSITV VSNANRLLRF KPKE |
|---|---|
| Length |
834 |
| Mol. Wt |
87.872 kDa |
| pI |
5.4 (calculated) |
| Extinction coefficient |
69,900 - 70,650 (calc based on 10 Y, 10 W, and 6 C residues) |
| edit table |
Domains/Motifs/Modification Sites
|
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/>
|
|
Structure
|
Structure figures
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
| Resource type | Source | Notes/Reference |
|---|---|---|
|
Purification protocol |
| |
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
| ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.00 5.01 5.02 5.03 5.04 5.05 5.06 5.07 5.08 5.09 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 6.00 6.01 6.02 6.03 6.04 6.05 6.06 6.07 6.08 6.09 6.10 6.11 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
- ↑ 7.0 7.1 7.2 7.3 7.4 7.5 Fan, B & Rosen, BP (2002) Biochemical characterization of CopA, the Escherichia coli Cu(I)-translocating P-type ATPase. J. Biol. Chem. 277 46987-92 PubMed
- ↑ 8.0 8.1 8.2 8.3 Rensing, C et al. (2000) CopA: An Escherichia coli Cu(I)-translocating P-type ATPase. Proc. Natl. Acad. Sci. U.S.A. 97 652-6 PubMed
- ↑ 9.0 9.1 Petersen, C & Møller, LB (2000) Control of copper homeostasis in Escherichia coli by a P-type ATPase, CopA, and a MerR-like transcriptional activator, CopR. Gene 261 289-98 PubMed
- ↑ 10.0 10.1 10.2 10.3 10.4 10.5 10.6 10.7 10.8 Krogh, A et al. (2001) Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. J. Mol. Biol. 305 567-80 PubMed
- ↑ 11.0 11.1 11.2 11.3 11.4 11.5 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
- ↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
Categories
- GO:0005507 ! copper ion binding
- GO:0004008 ! copper-exporting ATPase activity
- GO:0005375 ! copper ion transmembrane transporter activity
- GO:0003824 ! catalytic activity
- GO:0000287 ! magnesium ion binding
- GO:0000166 ! nucleotide binding
- GO:0016020 ! membrane
- GO:0015992 ! proton transport
- GO:0005524 ! ATP binding
- GO:0006810 ! transport
- GO:0006811 ! ion transport
- GO:0006825 ! copper ion transport
- GO:0008152 ! metabolic process
- GO:0015662 ! ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism
- GO:0016021 ! integral component of membrane
- GO:0016787 ! hydrolase activity
- GO:0046873 ! metal ion transmembrane transporter activity
- GO:0016820 ! hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances
- GO:0030001 ! metal ion transport
- GO:0046872 ! metal ion binding
- GO:0031224 ! intrinsic component of membrane
- GO:0005886 ! plasma membrane
- GO:0006754 ! ATP biosynthetic process
- GO:0010273 ! detoxification of copper ion
- GO:0043682 ! copper-transporting ATPase activity
- GO:0016887 ! ATPase activity
- TMHMM Prediction
- Proteins
- GO:0016021 ! integral to membrane