metG:Gene Product(s): Difference between revisions

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F
F
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Seeded from EcoCyc
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complete
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GO:0005624
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membrane fraction
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PMID:16858726
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IDA: Inferred from Direct Assay
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C
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Seeded from EcoCyc
Seeded from EcoCyc

Revision as of 02:36, 22 May 2009

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

MetG

Synonyms

methionyl-tRNA synthetase[1], B2114[2][1], MetG[2][1], MetRS[2][1]

Product description

methionyl-tRNA synthetase[2][3];

Component of methionyl-tRNA synthetase[2][3]

Methionine--tRNA ligase[4]

EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference(s) Evidence Code with/from Aspect Notes Status
GO:0005515

protein binding

PMID:16606699[5]

IPI: Inferred from Physical Interaction

UniProtKB:P60422

F

Seeded from EcoCyc [6]

complete

GO:0005624

membrane fraction

PMID:16858726[7]

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc [6]

complete

GO:0000049

tRNA binding

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR002547
SP_KW:KW-0820

F

Seeded from EcoCyc [6]

complete

GO:0000166

nucleotide binding

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR001412
InterPro:IPR002304
InterPro:IPR004495
InterPro:IPR009080
InterPro:IPR014758
InterPro:IPR015413
SP_KW:KW-0547

F

Seeded from EcoCyc [6]

complete

GO:0003723

RNA binding

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0694

F

Seeded from EcoCyc [6]

complete

GO:0004812

aminoacyl-tRNA ligase activity

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR001412
InterPro:IPR009080
InterPro:IPR015413
SP_KW:KW-0030

F

Seeded from EcoCyc [6]

complete

GO:0004825

methionine-tRNA ligase activity

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000003
GO_REF:0000020

IEA: Inferred from Electronic Annotation

InterPro:IPR002304
InterPro:IPR004495
InterPro:IPR014758
EC:6.1.1.10
HAMAP:MF_00098

F

Seeded from EcoCyc 12.5 [8]

complete

GO:0005524

ATP binding

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000004
GO_REF:0000020

IEA: Inferred from Electronic Annotation

InterPro:IPR001412
InterPro:IPR002304
InterPro:IPR004495
InterPro:IPR009080
InterPro:IPR014758
InterPro:IPR015413
SP_KW:KW-0067
HAMAP:MF_00098

F

Seeded from EcoCyc 12.5 [8]

complete

GO:0005737

cytoplasm

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR001412
InterPro:IPR002304
InterPro:IPR004495
InterPro:IPR009080
InterPro:IPR014758
InterPro:IPR015413
SP_KW:KW-0963

C

Seeded from EcoCyc 12.5 [8]

complete

GO:0006412

translation

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR001412
InterPro:IPR002304
InterPro:IPR004495
InterPro:IPR009080
InterPro:IPR014758
InterPro:IPR015413
SP_KW:KW-0648

P

Seeded from EcoCyc [6]

complete

GO:0006418

tRNA aminoacylation for protein translation

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001412
InterPro:IPR009080
InterPro:IPR015413

P

Seeded from EcoCyc [6]

complete

GO:0006431

methionyl-tRNA aminoacylation

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000020

IEA: Inferred from Electronic Annotation

InterPro:IPR002304
InterPro:IPR004495
InterPro:IPR014758
HAMAP:MF_00098

P

Seeded from EcoCyc 12.5 [8]

complete

GO:0008270

zinc ion binding

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0862

F

Seeded from EcoCyc [6]

complete

GO:0016874

ligase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0436

F

Seeded from EcoCyc [6]

complete

GO:0046872

metal ion binding

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0479

F

Seeded from EcoCyc [6]

complete

GO:0005739

mitochondrion

PMID:16858726[7]

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc [6]

complete

GO:0005829

cytosol

PMID:16858726[7]

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc [6]

complete

GO:0000049

tRNA binding

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR002547
SP_KW:KW-0820

F

Seeded from EcoCyc

complete

GO:0000166

nucleotide binding

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR001412
InterPro:IPR002304
InterPro:IPR004495
InterPro:IPR009080
InterPro:IPR014758
InterPro:IPR015413
SP_KW:KW-0547

F

Seeded from EcoCyc

complete

GO:0003723

RNA binding

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0694

F

Seeded from EcoCyc

complete

GO:0004812

aminoacyl-tRNA ligase activity

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR001412
InterPro:IPR009080
InterPro:IPR015413
SP_KW:KW-0030

F

Seeded from EcoCyc

complete

GO:0004825

methionine-tRNA ligase activity

GO_REF:0000020
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000003

IEA: Inferred from Electronic Annotation

HAMAP:MF_00098
InterPro:IPR002304
InterPro:IPR004495
InterPro:IPR014758
EC:6.1.1.10

F

Seeded from EcoCyc

complete

GO:0005515

protein binding

PMID:16606699[5]

IPI: Inferred from Physical Interaction

UniProtKB:P60422

F

Seeded from EcoCyc

complete

GO:0005524

ATP binding

GO_REF:0000020
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

HAMAP:MF_00098
InterPro:IPR001412
InterPro:IPR002304
InterPro:IPR004495
InterPro:IPR009080
InterPro:IPR014758
InterPro:IPR015413
SP_KW:KW-0067

F

Seeded from EcoCyc

complete

GO:0005624

membrane fraction

PMID:16858726[7]

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of methionyl-tRNA synthetase

could be indirect

Protein

rplB

PMID:16606699[5]

Experiment(s):EBI-1141964

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Notes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc

MTQVAKKILV TCALPYANGS IHLGHMLEHI QADVWVRYQR MRGHEVNFIC ADDAHGTPIM
LKAQQLGITP EQMIGEMSQE HQTDFAGFNI SYDNYHSTHS EENRQLSELI YSRLKENGFI
KNRTISQLYD PEKGMFLPDR FVKGTCPKCK SPDQYGDNCE VCGATYSPTE LIEPKSVVSG
ATPVMRDSEH FFFDLPSFSE MLQAWTRSGA LQEQVANKMQ EWFESGLQQW DISRDAPYFG
FEIPNAPGKY FYVWLDAPIG YMGSFKNLCD KRGDSVSFDE YWKKDSTAEL YHFIGKDIVY
FHSLFWPAML EGSNFRKPSN LFVHGYVTVN GAKMSKSRGT FIKASTWLNH FDADSLRYYY
TAKLSSRIDD IDLNLEDFVQ RVNADIVNKV VNLASRNAGF INKRFDGVLA SELADPQLYK
TFTDAAEVIG EAWESREFGK AVREIMALAD LANRYVDEQA PWVVAKQEGR DADLQAICSM
GINLFRVLMT YLKPVLPKLT ERAEAFLNTE LTWDGIQQPL LGHKVNPFKA LYNRIDMRQV
EALVEASKEE VKAAAAPVTG PLADDPIQET ITFDDFAKVD LRVALIENAE FVEGSDKLLR
LTLDLGGEKR NVFSGIRSAY PDPQALIGRH TIMVANLAPR KMRFGISEGM VMAAGPGGKD
IFLLSPDAGA KPGHQVK
Length

677

Mol. Wt

76.25 kDa (calc) [2]

pI
Extinction coefficient

96260 - 97260 (calc based on 24 Y, 11 W, and 8 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes

Domain

575 - 677

tRNA-binding

UniProt:P00959

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<motif_map/>

Structure See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure>metG 562</beststructure>

Models

View models at:

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:METG-MONOMER

SwissModel (EcoliWiki Page)

SwissModel:P00959

UniProt (EcoliWiki Page)

UniProt:P00959

PFAM (EcoliWiki Page)

PFAM:PF00133

RefSeq (EcoliWiki Page)

RefSeq:NP_416617

ModBase (EcoliWiki Page)

ModBase:P00959

PDB (EcoliWiki Page)

PDB:1F4L


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Notes

Links

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Name URL Comments
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References

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  1. ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  6. ↑ 6.00 6.01 6.02 6.03 6.04 6.05 6.06 6.07 6.08 6.09 6.10 6.11 6.12 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  7. ↑ 7.0 7.1 7.2 7.3 Lasserre, JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27 3306-21 PubMed
  8. ↑ 8.0 8.1 8.2 8.3 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7


Categories

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