Phage lambda cI:Gene Product(s): Difference between revisions

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The structure of the N-terminal domain of lambda repressor in a cocrystal with operator DNA was described by Beamer and Pabo<ref name='PMID:1387915'/>. DNA recognition is via a helix-turn-helix DNA binding motif {{:Phage_lambda_cI:Gene_Product(s)/highlight_hth}}that contacts operator DNA in the major groove.  Additonal contacts are made by an N-terminal arm {{:Phage_lambda_cI:Gene_Product(s)/highlight_arm}}that reaches around the DNA.
The structure of the N-terminal domain of lambda repressor in a cocrystal with operator DNA was described by Beamer and Pabo<ref name='PMID:1387915'/>. DNA recognition is via a helix-turn-helix DNA binding motif {{:Phage_lambda_cI:Gene_Product(s)/highlight_hth}}that contacts operator DNA in the major groove.  Additonal contacts are made by an N-terminal arm {{:Phage_lambda_cI:Gene_Product(s)/highlight_arm}}that reaches around the DNA.
The N-terminal domain forms weak homodimers via helix 5-helix 5 contacts {{:Phage_lambda_cI:Gene_Product(s)/highlight_h5}}<ref name='PMID:3388019'/>.


==Gene Product Resources==
==Gene Product Resources==

Revision as of 06:26, 13 April 2009

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

Standard name

cI

Synonyms

cI, lambdap88

Product description

repressor

EC number (for enzymes)
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Notes

Function

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Gene Ontology

Qualifier GO ID GO term name Reference(s) Evidence Code with/from Aspect Notes Status
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Interactions


Notes

Localization

Compartment Description Evidence Notes
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Notes

Structure and Physical Properties

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Physical Properties

Sequence 
MSTKKKPLTQ EQLEDARRLK AIYEKKKNEL GLSQESVADK MGMGQSGVGA LFNGINALNA
YNAALLAKIL KVSVEEFSPS IAREIYEMYE AVSMQPSLRS EYEYPVFSHV QAGMFSPELR
TFTKGDAERW VSTTKKASDS AFWLEVEGNS MTAPTGSKPS FPDGMLILVD PEQAVEPGDF
CIARLGGDEF TFKKLIRDSG QVFLQPLNPQ YPMIPCNESC SVVGKVIASQ WPEETFG
Mol. Wt

26.21 kDa (calc)

pI
Extinction coefficient
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Domains/Motifs/Modification Sites

Type Residues Description Notes
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Structure

See Help:Jmol in EcoliWiki

Lambda repressor N-terminal domain/operator cocrystal PDB:1LMB
<jmol> <jmolApplet> <color>white</color> <name>1LMB</name> <uploadedFileContents >1LMB.pdb</uploadedFileContents> <script> wireframe off; spacefill off; trace off; spacefill on; rotate ON; color chain; hide water; select *:1, *:2; color cpk; </script> </jmolApplet> </jmol>
<jmol> <jmolCheckbox > <scriptWhenChecked > rotate on; </scriptWhenChecked > <scriptWhenUnchecked > rotate off; </scriptWhenUnchecked > <checked>true</checked> <text>rotate</text> </jmolCheckbox > <jmolCheckbox> <scriptWhenChecked > hide none; hide hoh; </scriptWhenChecked > <scriptWhenUnchecked > hide *:1,*:2; </scriptWhenUnchecked > <checked>true</checked> <text>DNA</text> </jmolCheckbox > <jmolCheckbox > <scriptWhenChecked > hide none; hide hoh; </scriptWhenChecked > <scriptWhenUnchecked > hide *:3, *:4; </scriptWhenUnchecked > <checked>true</checked> <text>Protein</text> </jmolCheckbox > <jmolButton><script>reset; wireframe off; spacefill off; trace off; hide water; spacefill on; rotate ON; color chain; select *:1, *:2; color cpk; </script><text>reset</text></jmolButton> </jmol>

Coming soon...

  • A new Structure table.
    We are currently working on reformatting the table to provide more information and references to structures and computational models. We would love to add your research here! If you are interested in adding your data, contact Daniel. You can find our [Blast results here].

See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

X-ray

X-ray

Models

lambda


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Notes

The structure of the N-terminal domain of lambda repressor in a cocrystal with operator DNA was described by Beamer and Pabo[1]. DNA recognition is via a helix-turn-helix DNA binding motif Phage lambda cI:Gene Product(s)/highlight hththat contacts operator DNA in the major groove. Additonal contacts are made by an N-terminal arm <jmol> <jmolLink> <target>1LMB</target> <script> select 1-6:3, 1-6:4; color yellow; </script> <text>(highlight arm)</text> </jmolLink> </jmol>that reaches around the DNA.

The N-terminal domain forms weak homodimers via helix 5-helix 5 contacts <jmol> <jmolLink> <target>1LMB</target> <script> select *:3,*:4; spacefill off; cartoon; select 84,87; spacefill on; select *; hide hoh; hide *:1,*:2; zoomto (84:4) 300; </script> <text>(highlight)</text> </jmolLink> </jmol>[2].

Gene Product Resources

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Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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Database Accession Notes

Genbank (EcoliWiki Page)

Genbank:NP_040628.1

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Notes

Links

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Name URL Comments
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References

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  1. ↑ Beamer, LJ & Pabo, CO (1992) Refined 1.8 A crystal structure of the lambda repressor-operator complex. J. Mol. Biol. 227 177-96 PubMed
  2. ↑ Reidhaar-Olson, JF & Sauer, RT (1988) Combinatorial cassette mutagenesis as a probe of the informational content of protein sequences. Science 241 53-7 PubMed


Categories

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