dnaX:Expression: Difference between revisions
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The amount of gamma is estimated to be about 20 molecules per cell, based on an estimate of 10 dimers<ref name='PMID:6254978'/>. However, while gamma purifies as a dimer, it is present as one subunit in the τ<sub>2</sub>γδδ'χψ DnaX complex. | The amount of gamma is estimated to be about 20 molecules per cell, based on an estimate of 10 dimers<ref name='PMID:6254978'/>. However, while gamma purifies as a dimer, it is present as one subunit in the τ<sub>2</sub>γδδ'χψ DnaX complex. | ||
==Intracellular Levels== | |||
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==Transcription and Transcriptional Regulation== | ==Transcription and Transcriptional Regulation== | ||
Revision as of 22:23, 17 March 2009
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Overview | Transcription | Translation | Turnover | Experimental | Links | References | Suggestions |
Overview
This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
dnaX encodes two gene products from the same gene. A programmed frameshift is needed to generate the gamma subunit[1][2][3].
The amount of gamma is estimated to be about 20 molecules per cell, based on an estimate of 10 dimers[4]. However, while gamma purifies as a dimer, it is present as one subunit in the τ2γδδ'χψ DnaX complex.
Intracellular Levels
| Molecule | Organism or Strain | Molecules per cell | Growth conditions | Growth rate | Assay used | Notes | Reference(s) |
|---|---|---|---|---|---|---|---|
| edit table |
Notes
Transcription and Transcriptional Regulation
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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.
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Notes
This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Translation and Regulation of Translation
This picture shows the sequence around the N-terminus.
Notes
This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Turnover and Regulation of Turnover
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Notes
This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.
Experimental
Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.
| Allele Name | Mutation | Phenotype | Reference |
|---|---|---|---|
| edit table |
Expression Studies
See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.
| Type | Reference | Notes |
|---|---|---|
|
microarray |
NCBI GEO profiles for dnaX | |
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microarray |
Summary of data for dnaX from multiple microarray studies | |
| edit table |
Expression Resources
See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.
| Resource Name | Resource Type | Description | Source | Notes |
|---|---|---|---|---|
|
GFP Fusion |
Intergenic region (491102..491347) fused to gfpmut2. |
GFP fusion described in Zaslaver, et al. | ||
| edit table |
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Notes
Accessions Related to dnaX Expression
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
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| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
- ↑ Tsuchihashi, Z & Kornberg, A (1990) Translational frameshifting generates the gamma subunit of DNA polymerase III holoenzyme. Proc. Natl. Acad. Sci. U.S.A. 87 2516-20 PubMed
- ↑ Blinkowa, AL & Walker, JR (1990) Programmed ribosomal frameshifting generates the Escherichia coli DNA polymerase III gamma subunit from within the tau subunit reading frame. Nucleic Acids Res. 18 1725-9 PubMed
- ↑ Flower, AM & McHenry, CS (1990) The gamma subunit of DNA polymerase III holoenzyme of Escherichia coli is produced by ribosomal frameshifting. Proc. Natl. Acad. Sci. U.S.A. 87 3713-7 PubMed
- ↑ Hübscher, U & Kornberg, A (1980) The dnaZ protein, the gamma subunit of DNA polymerase III holoenzyme of Escherichia coli. J. Biol. Chem. 255 11698-703 PubMed
- ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed
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