ais:Gene: Difference between revisions

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!{{table_heading_style}} |Standard name
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''ais''  
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!{{table_heading_style}} |Mnemonic
!align=left {{table_heading_style}} |Mnemonic
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!{{table_heading_style}} |Synonyms
!align=left {{table_heading_style}} |Synonyms
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''ECK2245, b2252, JW2246''<ref name='LIB:Riley_2006'/>
''ECK2245, b2252, JW2246''<ref name='LIB:Riley_2006'/>
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!Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
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MG1655
MG1655
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{{GBrowseFastaLink|ecoli|NC_000913|2363040|2363642|FASTA Dump}}
{{GBrowseFastaLink|ecoli|NC_000913|2363040|2363642|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=G7165 EcoCyc Sequence Dump]  
*[http://biocyc.org/ECOLI/sequence?object=G7165 EcoCyc Sequence Dump]  
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W3110
W3110
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{{GBrowseFastaLink|ecoli_W3110|AC_000091|2369688|2370290|FASTA Dump}}  
{{GBrowseFastaLink|ecoli_W3110|AC_000091|2369688|2370290|FASTA Dump}}  
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|[[PMID:16738554]]<ref name=PMID:16738554/>
[[PMID:16738554]]<ref name=PMID:16738554/>
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[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=108092 CGSC9811]<ref name='LIB:cgsc'/>  
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=108092 CGSC9811]<ref name='LIB:cgsc'/>  
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|[[PMID:16738554]]<ref name=PMID:16738554/>
[[PMID:16738554]]<ref name=PMID:16738554/>
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[[cgscmutation:99958|CGSC:99958]]
[[cgscmutation:99958|CGSC:99958]]
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!Resource!!Resource Type!!Source!!Notes/Reference
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JW2246
JW2246
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[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
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|[[PMID:16769691]]<ref name=PMID:16769691/>
[[PMID:16769691]]<ref name=PMID:16769691/>


Status:Clone OK
Status:Clone OK
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Primer 2:CCGTGGTTAACGAATTCCCCATC  
Primer 2:CCGTGGTTAACGAATTCCCCATC  
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[[yfaL:Gene|zfa-723::Tn10]]
[[yfaL:Gene|zfa-723::Tn10]]
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CAG12178 gyrA+ = [http://cgsc2.biology.yale.edu/Strain.php?ID=44155 CGSC7526]<ref name='LIB:cgsc'/>
CAG12178 gyrA+ = [http://cgsc2.biology.yale.edu/Strain.php?ID=44155 CGSC7526]<ref name='LIB:cgsc'/>
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est. P1 cotransduction: 48% <ref name='LIB:Nichols_linked_Tn'/>
est. P1 cotransduction: 48% <ref name='LIB:Nichols_linked_Tn'/> <br />
 
Synonyms:zei-723::Tn10, zfa-723::Tn10 nnnThe original CAG12178 was NalR.  This is a NalS derivative constructed by S. McAteer (CGSC).  
Synonyms:zei-723::Tn10, zfa-723::Tn10  
|-  
 
 
The original CAG12178 was NalR.  This is a NalS derivative constructed by S. McAteer (CGSC).  
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[[yfbO:Gene|zfb-223::Tn10]]
[[yfbO:Gene|zfb-223::Tn10]]
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CAG18484 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40073 CGSC7406]<ref name='LIB:cgsc'/>
CAG18484 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40073 CGSC7406]<ref name='LIB:cgsc'/>
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est. P1 cotransduction: 37% <ref name='LIB:Nichols_linked_Tn'/>
est. P1 cotransduction: 37% <ref name='LIB:Nichols_linked_Tn'/> <br />
Synonyms:zej-223::Tn10


Synonyms:zej-223::Tn10
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[https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]])
[https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]])
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[http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0007451&formSubmitReturn=1 ASAP:ABE-0007451]
[http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0007451&formSubmitReturn=1 ASAP:ABE-0007451]
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Revision as of 18:25, 29 January 2009

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

ais

Mnemonic
Synonyms

ECK2245, b2252, JW2246[1]

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

50.93 minutes 

MG1655: 2363642..2363040
gbrowse_img.php?coord=2362040..2364642&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 2370290..2369688
gbrowse_img.php?coord=2368688..2371290&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature type Location Description
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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Δais (Keio:JW2246)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC9811[3]

Δais-733::kan

PMID:16738554[2]

CGSC:99958


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2246

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCTTAGCTTTTTGCCGCTCTTC

Primer 2:CCGTGGTTAACGAATTCCCCATC

zfa-723::Tn10

Linked marker

CAG12178 gyrA+ = CGSC7526[3]

est. P1 cotransduction: 48% [5]
Synonyms:zei-723::Tn10, zfa-723::Tn10 nnnThe original CAG12178 was NalR. This is a NalS derivative constructed by S. McAteer (CGSC).

zfb-223::Tn10

Linked marker

CAG18484 = CGSC7406[3]

est. P1 cotransduction: 37% [5]
Synonyms:zej-223::Tn10

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:G7165

EchoBASE (EcoliWiki Page)

EchoBASE:EB2949

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2252

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C2794

EcoGene (EcoliWiki Page)

EcoGene:EG13155

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:AIS

ASAP (EcoliWikiPage)

ASAP:ABE-0007451


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Notes

Links

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Name URL Comments
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See Help:Links_table for how to enter or edit information in this section of EcoliWiki.

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. ↑ 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).


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