thrL:Gene: Difference between revisions

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CAG18442 = [http://cgsc2.biology.yale.edu/Strain.php?ID=38985 CGSC7320]<ref name='LIB:cgsc'/>
CAG18442 = [http://cgsc2.biology.yale.edu/Strain.php?ID=38985 CGSC7320]<ref name='LIB:cgsc'/>
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est. P1 cotransduction: 99% <ref name='LIB:Nichols_linked_Tn'/>nnSynonyms:thr-34::Tn10  
est. P1 cotransduction: 99% <ref name='LIB:Nichols_linked_Tn'/> <br />
Synonyms:thr-34::Tn10  
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CAG12093 = [http://cgsc2.biology.yale.edu/Strain.php?ID=38008 CGSC7322]<ref name='LIB:cgsc'/>
CAG12093 = [http://cgsc2.biology.yale.edu/Strain.php?ID=38008 CGSC7322]<ref name='LIB:cgsc'/>
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est. P1 cotransduction: 28% <ref name='LIB:Nichols_linked_Tn'/>nnSynonyms:car-96::Tn10  
est. P1 cotransduction: 28% <ref name='LIB:Nichols_linked_Tn'/> <br />
Synonyms:car-96::Tn10  


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Revision as of 17:38, 29 January 2009

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

thrL

Mnemonic
Synonyms

ECK0001, b0001, JW4367[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

0 minutes 

MG1655: 190..255
gbrowse_img.php?coord=0..1255&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 190..255
gbrowse_img.php?coord=0..1255&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature type Location Description
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔthrL (Keio:JW4367)

deletion

deletion

PMID:16738554[2]

Shigen

ΔthrL721::kan

PMID:16738554[2]

CGSC:103154


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

Plasmid clone

Shigen

PMID:16769691[3]

Status:

Primer 1:

Primer 2:

thrA34::Tn10

Linked marker

CAG18442 = CGSC7320[4]

est. P1 cotransduction: 99% [5]
Synonyms:thr-34::Tn10

carB96::Tn10

Linked marker

CAG12093 = CGSC7322[4]

est. P1 cotransduction: 28% [5]
Synonyms:car-96::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11277

EchoBASE (EcoliWiki Page)

EchoBASE:EB1255

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0001

EcoGene (EcoliWiki Page)

EcoGene:EG11277

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:THRL

ASAP (EcoliWikiPage)

ASAP:ABE-0000006

ASAP (EcoliWikiPage)

ASAP:ABE-0000006


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Notes

Links

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Name URL Comments


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References

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  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  4. ↑ 4.0 4.1 CGSC: The Coli Genetics Stock Center
  5. ↑ 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).


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