rsmF:Gene Product(s): Difference between revisions
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Purified recombinant YebU protein retains its specificity for C1407 in vitro, | Purified recombinant YebU protein retains its specificity for C1407 in vitro, | ||
and methylates 30 S subunits (but not naked 16 S rRNA or 70 S ribosomes) | and methylates 30 S subunits (but not naked 16 S rRNA or 70 S ribosomes) | ||
isolated from yebU knockout strains.<ref name='PMID:16678201'/>. Andersen and Douthwaite | isolated from yebU knockout strains.<ref name='PMID:16678201'/>. Andersen and Douthwaite suggest that a more | ||
appropriate designation for yebU would be the rRNA small subunit | appropriate designation for yebU would be the rRNA small subunit | ||
methyltransferase gene rsmF. | methyltransferase gene rsmF. | ||
Revision as of 16:36, 13 November 2008
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
| Standard name |
YebU product, m5C methyltransferase |
|---|---|
| Synonyms |
predicted methyltransferase product[1], B1835 product[2][1], YebU product[2][1], RsmF product[2][1] |
| Product description |
16S rRNA m5C1407 specific methyltransferase[3][2][4]. Inactivation of the yebU gene leads to loss of methylation at C1407 in 16 S rRNA, but does not interfere with methylation at C1962 in 23 S rRNA. Purified recombinant YebU protein retains its specificity for C1407 in vitro, and methylates 30 S subunits (but not naked 16 S rRNA or 70 S ribosomes) isolated from yebU knockout strains.[3]. Andersen and Douthwaite suggest that a more appropriate designation for yebU would be the rRNA small subunit methyltransferase gene rsmF. |
| EC number (for enzymes) |
|
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Notes
Function
Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference(s) | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0031167 |
rRNA methylation |
P |
Seeded from EcoCyc 11.1[4]. |
required fields missing | ||||
| GO:0016434 |
rRNA (cytosine) methyltransferase activity |
F |
Seeded from EcoCyc 11.1[4]. |
required fields missing | ||||
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Interactions
| Partner Type | Partner | Notes |
|---|---|---|
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Notes
Localization
| Compartment | Description | Evidence | Notes |
|---|---|---|---|
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Notes
Structure and Physical Properties
Physical Properties
| Sequence |
MLVAQHTVYF PDAFLTQMRE AMPSTLSFDD FLAACQRPLR RSIRVNTLKI SVADFLQLTA PYGWTLTPIP WCEEGFWIER DNEDALPLGS TAEHLSGLFY IQEASSMLPV AALFADGNAP QRVMDVAAAP GSKTTQISAR MNNEGAILAN EFSASRVKVL HANISRCGIS NVALTHFDGR VFGAAVPEMF DAILLDAPCS GEGVVRKDPD ALKNWSPESN QEIAATQREL IDSAFHALRP GGTLVYSTCT LNQEENEAVC LWLKETYPDA VEFLPLGDLF PGANKALTEE GFLHVFPQIY DCEGFFVARL RKTQAIPALP APKYKVGNFP FSPVKDREAG QIRQAATGVG LNWDENLRLW QRDKELWLFP VGIEALIGKV RFSRLGIKLA ETHNKGYRWQ HEAVIALASP DNMNAFELTP QEAEEWYRGR DVYPQAAPVA DDVLVTFQHQ PIGLAKRIGS RLKNSYPREL VRDGKLFTGN A |
|---|---|
| Mol. Wt |
53.23 kDa (calc) [2] |
| pI | |
| Extinction coefficient |
71390 - 72265 (calc based on 11 Y, 10 W, and 7 C residues) |
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Domains/Motifs/Modification Sites
| Type | Residues | Description | Notes |
|---|---|---|---|
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Structure
| Structures |
<beststructure>rsmF 562</beststructure> |
|---|---|
| Models |
View models at: |
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Notes
Gene Product Resources
| Resource type | Source | Notes/Reference |
|---|---|---|
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Notes
Accessions in Other Databases
| Database | Accession | Notes |
|---|---|---|
|
| ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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See Help:Links_table for how to enter or edit information in this section of EcoliWiki.
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 Andersen, NM & Douthwaite, S (2006) YebU is a m5C methyltransferase specific for 16 S rRNA nucleotide 1407. J. Mol. Biol. 359 777-86 PubMed
- ↑ 4.0 4.1 4.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
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