rhaD:Gene: Difference between revisions
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Primer 2:CCaAGCGCCAGCGCACTGGCGAG | Primer 2:CCaAGCGCCAGCGCACTGGCGAG | ||
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[[yihO:Gene|zih-35::Tn10]] | |||
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Linked marker | |||
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CAG18495 = [http://cgsc2.biology.yale.edu/Strain.php?ID=37140 CGSC7467]<ref name='LIB:cgsc'/> | |||
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est. P1 cotransduction: 35% <ref name='LIB:Nichols_linked_Tn'/> | |||
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[[pflD:Gene|pflD501::Tn10]] | |||
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Linked marker | |||
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CAG18477 = [http://cgsc2.biology.yale.edu/Strain.php?ID=37152 CGSC7470]<ref name='LIB:cgsc'/> | |||
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est. P1 cotransduction: 9% <ref name='LIB:Nichols_linked_Tn'/> | |||
Synonyms:zij-501::Tn10 | |||
CAG18477 also carries metF79(Am) (CGSC). | |||
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Revision as of 23:40, 28 July 2007
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Alleles and Phenotypes | Genetic Resources | Accessions | Links | References | Suggestions |
Nomenclature
| Standard name |
rhaD |
|---|---|
| Mnemonic | |
| Synonyms |
ECK3895, b3902, JW3873[1] |
| edit table | |
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
Notes
Location(s) and DNA Sequence
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
88.18 minutes, 88.18 minutes |
MG1655: 4092295..4091471 |
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|
W3110 |
|
W3110: 3542409..3543233 |
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| edit table |
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
Notes
Sequence Features
| Feature type | Location | Description |
|---|---|---|
| edit table |
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
Notes
Alleles and Phenotypes
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability |
|---|---|---|---|---|---|---|
|
ΔrhaD (Keio:) |
deletion |
deletion |
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| edit table |
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
Notes
Genetic Resources
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW3873 |
Plasmid clone |
Status:Clone OK Primer 1:GCCCAAAACATTACTCAGTCCTG Primer 2:CCaAGCGCCAGCGCACTGGCGAG | |
|
Linked marker |
est. P1 cotransduction: 35% [5] | ||
|
Linked marker |
est. P1 cotransduction: 9% [5] Synonyms:zij-501::Tn10
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| edit table |
See Help:Gene_resources for help entering information into the Genetic Resources table.
Notes
Accessions in Other Databases
| Database | Accession | Notes |
|---|---|---|
| edit table |
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
See Help:Links_table for how to enter or edit information in this section of EcoliWiki.
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 4.0 4.1 CGSC: The Coli Genetics Stock Center
- ↑ 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).