BL21(DE3): Difference between revisions
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==Sources == | ==Sources == | ||
[http://bccm.belspo.be/catalogues/genecorner-hosts BCCM/GeneCorner Plasmid Collection] | |||
==Chromosomal Genotype == | ==Chromosomal Genotype == | ||
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==Notes== | ==Notes== | ||
The strain contains DE3, a λ prophage carrying the T7 RNA polymerase gene under control of the lac UV5 promoter and lacIq. IPTG is required to induce expression of the T7 RNA polymerase. Protein expression from transforming plasmids containing a T7 promoter-driven expression system is repressed until IPTG induction of T7 RNA polymerase expression occurs. | |||
The strain does not contain the lon protease and is also deficient in the outer membrane protease, OmpT. The lack of two key proteases reduces the degradation of heterologous proteinsexpressed in the strain | |||
==References== | ==References== | ||
Studier and Moffatt, J. Mol. Biol. 189 (1986), 113-130 [https://www.ncbi.nlm.nih.gov/pubmed/3537305 PMID: 3537305] | |||
__NOTOC__ | __NOTOC__ | ||
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[[Category:Expression Strains]] | [[Category:Expression Strains]] | ||
[[Category:Stratagene Strains]] | [[Category:Stratagene Strains]] | ||
[[Category:BCCM/GeneCorner]] | |||
Revision as of 13:50, 6 January 2020
See Help:Strains for more iformation about strains in EcoliWiki.
Alternate Names
Sources
BCCM/GeneCorner Plasmid Collection
Chromosomal Genotype
E. coli B dcm ompT hsdS(rB-mB-) gal
Plasmids
Phage
Phenotypes
Construction
Notes
The strain contains DE3, a λ prophage carrying the T7 RNA polymerase gene under control of the lac UV5 promoter and lacIq. IPTG is required to induce expression of the T7 RNA polymerase. Protein expression from transforming plasmids containing a T7 promoter-driven expression system is repressed until IPTG induction of T7 RNA polymerase expression occurs. The strain does not contain the lon protease and is also deficient in the outer membrane protease, OmpT. The lack of two key proteases reduces the degradation of heterologous proteinsexpressed in the strain
References
Studier and Moffatt, J. Mol. Biol. 189 (1986), 113-130 PMID: 3537305