Category:Strain:BW25113: Difference between revisions
mNo edit summary |
|||
| Line 53: | Line 53: | ||
== Notes == | == Notes == | ||
BW25113 is a derivative of the F<sup>-</sup>, λ<sup>-</sup>, ''E. coli'' K-12 strain [http://cgsc2.biology.yale.edu/Strain.php?ID=7024 BD792 (CGSC6159)]<ref name='PMID:10829079'/>, which is a derivative of ''E. coli'' K-12 W1485. According to the CGSC, BD792 carries ''rpoS396''(Am), but this mutation is not listed in the published genotype for BW25113. | BW25113 is a derivative of the F<sup>-</sup>, λ<sup>-</sup>, ''E. coli'' K-12 strain [http://cgsc2.biology.yale.edu/Strain.php?ID=7024 BD792 (CGSC6159)]<ref name='PMID:10829079'/>, which is a derivative of ''E. coli'' K-12 W1485. According to the CGSC, BD792 carries ''rpoS396''(Am), but this mutation is not listed in the published genotype for BW25113, as according to Baba 2006 <ref name=PMID:16738554/>, a pseudoreversion rpoS (Q33) allele was transferred from MG1655 into a predecessor of BW25113. | ||
BW25113 was used to make the Keio Collection of single-gene knockouts. <ref name=PMID:16738554/> | BW25113 was used to make the Keio Collection of single-gene knockouts. <ref name=PMID:16738554/> | ||
Revision as of 17:50, 5 June 2013
See Help:Strains for more information about strains in EcoliWiki.
| Name | |
|---|---|
| Synonyms | |
| Genotype | |
| Phenotype | |
| Plasmids | |
| Phage | |
| Sources | |
| Construction | |
| References |
|
| edit table |
Notes
BW25113 is a derivative of the F-, λ-, E. coli K-12 strain BD792 (CGSC6159)[1], which is a derivative of E. coli K-12 W1485. According to the CGSC, BD792 carries rpoS396(Am), but this mutation is not listed in the published genotype for BW25113, as according to Baba 2006 [2], a pseudoreversion rpoS (Q33) allele was transferred from MG1655 into a predecessor of BW25113.
BW25113 was used to make the Keio Collection of single-gene knockouts. [2] [1] Available from the Coli Genetics Stock Center: CGSC#7636 The genotype for BW25113 in Datsenko et al. [1] said the strain is lacIQ, but it was later shown to be lacI+ Baba et al. (Supplementary Table 1)[2].
According to the CGSC, lacZ4787(del) has 4 tandem copies of the rrnB transcriptional terminator inserted by gene replacement into the region extending from near the SacII site near the N-terminus of lacZ through the promoter.
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 Datsenko, KA & Wanner, BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97 6640-5 PubMed
- ↑ 2.0 2.1 2.2 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed