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est. P1 cotransduction: 34% <ref name='LIB:Nichols_linked_Tn'/> <br />  
est. P1 cotransduction: 34% <ref name='LIB:Nichols_linked_Tn'/> <br />  
Synonyms:zij-501::Tn10 nnnCAG18477 also carries metF79(Am) (CGSC).  
Synonyms:zij-501::Tn10 nnnCAG18477 also carries metF79(Am) (CGSC).  
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[[:Category:Kohara_Phage:4H12|4H12]]
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Kohara Phage
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=539 Genobase]
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PMID:3038334


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Revision as of 21:24, 2 September 2011

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

glpF

Mnemonic

Glycerol phosphate

Synonyms

ECK3919, b3927, JW3898[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

88.7 minutes, 88.7 minutes 

MG1655: 4116113..4115268
<gbrowseImage> name=NC_000913:4115268..4116113 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4097572..4096727
<gbrowseImage> name=NC_012967:4096727..4097572 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4004937..4005782
<gbrowseImage> name=NC_012759:4004937..4005782 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3518591..3519436
<gbrowseImage> name=AC_000091:3518591..3519436 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4215810..4214965
<gbrowseImage> name=NC_010473:4214965..4215810 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔglpF (Keio:JW3898)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC10813[3]

glpFV126I

V126I

(in strain: HB101)

Strain variation; seeded from UniProt:P0AER0

glpFM160I

M160I

(in strain: HB101)

Strain variation; seeded from UniProt:P0AER0

glpFG239S

G239S

(in strain: HB101)

Strain variation; seeded from UniProt:P0AER0

glpFPL236FW

PL236FW

No detectable water or glycerol permeability

seeded from UniProt:P0AER0

glpF17

CGSC:5657

ΔglpF786::kan

PMID:16738554[2]

CGSC:101186


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3898

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCAGTCAAACATCAACCTTGAA

Primer 2:CCaAGCGAAGCTTTTTGTTCTGA

zih-35::Tn10

Linked marker

CAG18495 = CGSC7467[3]

est. P1 cotransduction: 9% [5]

pflD501::Tn10

Linked marker

CAG18477 = CGSC7470[3]

est. P1 cotransduction: 34% [5]
Synonyms:zij-501::Tn10 nnnCAG18477 also carries metF79(Am) (CGSC).

4H12

Kohara Phage

Genobase

PMID:3038334[6]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10396

EchoBASE (EcoliWiki Page)

EchoBASE:EB0391

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3927

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C4879

EcoGene (EcoliWiki Page)

EcoGene:EG10396

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:GLPF

CGSC (EcoliWiki Page)

CGSC:692

ASAP (EcoliWikiPage)

ASAP:ABE-0012828


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. ↑ 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  6. ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed

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