glpF:Gene: Difference between revisions

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{{GBrowseLink|ecoli_W3110|AC_000091|3517591|3520436|GBrowse(W3110)}}  
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{{GBrowseLink|ecoli_DH10B|NC_010473|4213275|4217500|GBrowse(DH10B)}}
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Revision as of 20:01, 17 August 2011

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

glpF

Mnemonic

Glycerol phosphate

Synonyms

ECK3919, b3927, JW3898[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

88.7 minutes, 88.7 minutes 

MG1655: 4116113..4115268
<gbrowseImage> name=NC_000913:4115268..4116113 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4097572..4096727
<gbrowseImage> name=NC_012967:4096727..4097572 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4004937..4005782
<gbrowseImage> name=NC_012759:4004937..4005782 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3518591..3519436
<gbrowseImage> name=AC_000091:3518591..3519436 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4215810..4214965
<gbrowseImage> name=NC_010473:4214965..4215810 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔglpF (Keio:JW3898)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC10813[3]

glpFV126I

V126I

(in strain: HB101)

Strain variation; seeded from UniProt:P0AER0

glpFM160I

M160I

(in strain: HB101)

Strain variation; seeded from UniProt:P0AER0

glpFG239S

G239S

(in strain: HB101)

Strain variation; seeded from UniProt:P0AER0

glpFPL236FW

PL236FW

No detectable water or glycerol permeability

seeded from UniProt:P0AER0

glpF17

CGSC:5657

ΔglpF786::kan

PMID:16738554[2]

CGSC:101186


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3898

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCAGTCAAACATCAACCTTGAA

Primer 2:CCaAGCGAAGCTTTTTGTTCTGA

zih-35::Tn10

Linked marker

CAG18495 = CGSC7467[3]

est. P1 cotransduction: 9% [5]

pflD501::Tn10

Linked marker

CAG18477 = CGSC7470[3]

est. P1 cotransduction: 34% [5]
Synonyms:zij-501::Tn10 nnnCAG18477 also carries metF79(Am) (CGSC).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10396

EchoBASE (EcoliWiki Page)

EchoBASE:EB0391

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3927

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C4879

EcoGene (EcoliWiki Page)

EcoGene:EG10396

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:GLPF

CGSC (EcoliWiki Page)

CGSC:692

ASAP (EcoliWikiPage)

ASAP:ABE-0012828


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. ↑ 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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