ZYCY10P3S2T: Difference between revisions

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==Chromosomal Genotype==
[[araE|Cp8.araE]] [[endA|ΔendA]] [[lacY|bla.lacY A177C]] [[3BAD.I-SceI]] [[2BAD.ΦC31]] [[4BAD.Φ31]] [[4BAD.Φ31]]
<br>
araE driven by cp8 promoter <ref name='PMID:11739756'/> <br>
araE driven by cp8 promoter <ref name='PMID:11739756'/> <br>
lacY A177C: driven by bla promoter, L-arabinose transporter function <ref name='PMID:10811905'/> <br>
lacY A177C: driven by bla promoter, L-arabinose transporter function <ref name='PMID:10811905'/> <br>
ΦC31: driven by L-arabinose inducible araCBAD system, intergrase
ΦC31: driven by L-arabinose inducible araCBAD system, intergrase


==Plasmids==
==Phage==
==Phenotypes==
==Construction==


==Notes==
For minicircle plasmid preparation based on attB/attP-Φ31 recombination
For minicircle plasmid preparation based on attB/attP-Φ31 recombination
Parental background plasmid was removed by I-SceI endonuclease
Parental background plasmid was removed by I-SceI endonuclease


BW27783
BW27783
__NOTOC__


==References==
==References==

Revision as of 03:07, 2 July 2011

See Help:Strains for more information about strains in EcoliWiki.

Name
Synonyms
Genotype
Phenotype
Plasmids
Phage
Sources

SBI System Biosciences

Construction
References



Notes

See Help:Strains for more information about strains in EcoliWiki.


araE driven by cp8 promoter [1]
lacY A177C: driven by bla promoter, L-arabinose transporter function [2]
ΦC31: driven by L-arabinose inducible araCBAD system, intergrase


For minicircle plasmid preparation based on attB/attP-Φ31 recombination Parental background plasmid was removed by I-SceI endonuclease

BW27783

References

See Help:References for how to manage references in EcoliWiki.

  1. ↑ Khlebnikov, A et al. (2001) Homogeneous expression of the P(BAD) promoter in Escherichia coli by constitutive expression of the low-affinity high-capacity AraE transporter. Microbiology (Reading, Engl.) 147 3241-7 PubMed
  2. ↑ Yu, D et al. (2000) An efficient recombination system for chromosome engineering in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 97 5978-83 PubMed