ZYCY10P3S2T
See Help:Strains for more information about strains in EcoliWiki.
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| Name | |
|---|---|
| Synonyms | |
| Genotype |
Cp8.araE ΔendA bla.lacY A177C 3BAD.I-SceI 2BAD.ΦC31 4BAD.Φ31 4BAD.Φ31 |
| Phenotype | |
| Plasmids | |
| Phage | |
| Sources | |
| Construction | |
| References |
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| edit table |
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Notes
See Help:Strains for more information about strains in EcoliWiki.
araE driven by cp8 promoter [1]
lacY A177C: driven by bla promoter, L-arabinose transporter function [2]
ΦC31: driven by L-arabinose inducible araCBAD system, intergrase
For minicircle plasmid preparation based on attB/attP-Φ31 recombination
Parental background plasmid was removed by I-SceI endonuclease
BW27783
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Khlebnikov, A et al. (2001) Homogeneous expression of the P(BAD) promoter in Escherichia coli by constitutive expression of the low-affinity high-capacity AraE transporter. Microbiology (Reading, Engl.) 147 3241-7 PubMed
- ↑ Yu, D et al. (2000) An efficient recombination system for chromosome engineering in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 97 5978-83 PubMed
