arnA:Gene: Difference between revisions

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{|  id="C46a9d9b5afd06"  class=" tableEdit Gene_sequence_features_table"   
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!|Feature type!!Location!!Description
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes


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Revision as of 22:18, 10 May 2010

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

arnA

Mnemonic

ara4N (4-amino-4-deoxy-L-arabinose)

Synonyms

yfbG, ECK2248, b2255, JW2249, pmrI, SAF[1][2]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

51 minutes 

MG1655: 2366061..2368043
gbrowse_img.php?coord=2366061..2368043&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 2314552..2316534
gbrowse_img.php?coord=2314552..2316534&genome=REL606&name=NC_012967&trackset=0&width=300&.png

BW2952

NC_012759: 2251866..2253848
gbrowse_img.php?coord=2251866..2253848&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

W3110

 

W3110: 2372709..2374691
gbrowse_img.php?coord=2372709..2374691&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 2457049..2459031
gbrowse_img.php?coord=2457049..2459031&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

arnAR619M

R619M

400-fold lower activity

seeded from UniProt:P77398

arnAR619E,Y

R619E,Y

No activity

seeded from UniProt:P77398

arnAE434Q

E434Q

No activity

seeded from UniProt:P77398

arnAS433T

S433T

No activity

seeded from UniProt:P77398

arnAE434A

E434A

100-fold lower specific activity

seeded from UniProt:P77398

arnAD140A,N

D140A,N

Less than 1% residual activity

seeded from UniProt:P77398

arnAS433A

S433A

40-fold lower specific activity

seeded from UniProt:P77398

arnAH104K

H104K

Less than 1% residual activity

seeded from UniProt:P77398

arnAN102A

N102A

No activity

seeded from UniProt:P77398

arnAH104A

H104A

25-fold lower activity

seeded from UniProt:P77398

ΔarnA736::kan

PMID:16738554[3]

CGSC:106130


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

Plasmid clone

Shigen

PMID:16769691[4]

Status:

Primer 1:

Primer 2:

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:G7168

EchoBASE (EcoliWiki Page)

EchoBASE:EB3844

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2255

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C2797

EcoGene (EcoliWiki Page)

EcoGene:EG14091

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:Z3513

ASAP (EcoliWikiPage)

ASAP:ABE-0007460


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed

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