modE:Gene: Difference between revisions

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Partial loss of repression by modE
Partial loss of repression by modE
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seeded from [http://www.uniprot.org/uniprot/P0A9G8 UniProt:P0A9G8]
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modET125I
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T125I
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Transcription repression by modE even in the absence of molybdate
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modET125I
modEG133D
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T125I
G133D
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Revision as of 17:48, 18 February 2010

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

modE

Mnemonic
Synonyms

ECK0750, b0761, JW0744, modR, chlD[1]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

17.09 minutes 

MG1655: 793867..793079
gbrowse_img.php?coord=793079..793867&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 775273..774485
gbrowse_img.php?coord=774485..775273&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 795066..794278
gbrowse_img.php?coord=794278..795066&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 847795..847007
gbrowse_img.php?coord=847007..847795&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature type Location Description


Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔmodE (Keio:JW0744)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC8806[3]

modEA76V

A76V

Partial loss of repression by modE

seeded from UniProt:P0A9G8

modET125I

T125I

Transcription repression by modE even in the absence of molybdate

seeded from UniProt:P0A9G8

ΔmodE733::kan

PMID:16738554[2]

CGSC:101916

modEG133D

G133D

Transcription repression by modE even in the absence of molybdate

seeded from UniProt:P0A9G8


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0744

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCCAGGCCGAAATCCTTCTCAC

Primer 2:CCGCACAGCGTGGCGATAATCAC

nadA57::Tn10

Linked marker

CAG12147 = CGSC7351[3]

est. P1 cotransduction: 62% [5]

zbh-29::Tn10

Linked marker

CAG18493 = CGSC7353[3]

est. P1 cotransduction: 34% [5]
Synonyms:zbi-29::Tn10


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:G6395

EchoBASE (EcoliWiki Page)

EchoBASE:EB3017

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0761

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0837

EcoGene (EcoliWiki Page)

EcoGene:EG13227

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:MODE

ASAP (EcoliWikiPage)

ASAP:ABE-0002585



Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. ↑ 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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