arnA:Gene: Difference between revisions

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Less than 1% residual activity
Less than 1% residual activity
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seeded from [http://www.uniprot.org/uniprot/P77398 UniProt:P77398]
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arnAS433A
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S433A
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40-fold lower specific activity
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arnAS433A
arnAS433T
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S433A
S433T
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40-fold lower specific activity
No activity
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Revision as of 17:26, 18 February 2010

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

arnA

Mnemonic
Synonyms

yfbG, ECK2248, b2255, JW2249, pmrI, SAF[1][2]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

51 minutes 

MG1655: 2366061..2368043
gbrowse_img.php?coord=2366061..2368043&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 2314552..2316534
gbrowse_img.php?coord=2314552..2316534&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 2372709..2374691
gbrowse_img.php?coord=2372709..2374691&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 2457049..2459031
gbrowse_img.php?coord=2457049..2459031&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature type Location Description


Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

arnAD140A,N

D140A,N

Less than 1% residual activity

seeded from UniProt:P77398

arnAS433A

S433A

40-fold lower specific activity

seeded from UniProt:P77398

arnAH104K

H104K

Less than 1% residual activity

seeded from UniProt:P77398

arnAN102A

N102A

No activity

seeded from UniProt:P77398

arnAH104A

H104A

25-fold lower activity

seeded from UniProt:P77398

ΔarnA736::kan

PMID:16738554[3]

CGSC:106130

arnAS433T

S433T

No activity

seeded from UniProt:P77398


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

Plasmid clone

Shigen

PMID:16769691[4]

Status:

Primer 1:

Primer 2:


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:G7168

EchoBASE (EcoliWiki Page)

EchoBASE:EB3844

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2255

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C2797

EcoGene (EcoliWiki Page)

EcoGene:EG14091

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:Z3513

ASAP (EcoliWikiPage)

ASAP:ABE-0007460



Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed

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