msbA:Gene Product(s): Difference between revisions

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!align=left {{table heading style}} |Structures
!align=left {{table heading style}} |Structures
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<beststructure>msbA
<beststructure>
562</beststructure>
gene=msbA
 
taxon=562,83333
</beststructure>
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|-
!align=left {{table heading style}} |Models
!align=left {{table heading style}} |Models
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View models at:
View models at:
* [http://dragon.bio.purdue.edu/ecolpredict2/localsearch/cgi-bin/search3.cgi?q=msbA&sub=keywordSearc EcoliPredict]
* [http://dragon.bio.purdue.edu/ecolpredict2/localsearch/cgi-bin/search3.cgi?q=recA EcoliPredict]
* [http://modbase.compbio.ucsf.edu/modbase-cgi/model_search.cgi?searchmode=default&displaymode=moddetail&searchproperties=ALL&searchvalue=msbA&organism=562&organismtext= ModBase]
* [http://modbase.compbio.ucsf.edu/modbase-cgi/model_search.cgi?searchmode=default&displaymode=moddetail&searchproperties=ALL&searchvalue=recA&organism=562&organismtext= ModBase]
* [http://bioinf.cs.ucl.ac.uk/psipred/ PSI-PRED]
* [http://bioinf.cs.ucl.ac.uk/psipred/ PSI-PRED]
* [http://www.ebi.ac.uk/msd-srv/pqs/ Protein Quaternary Structure] (PQS)
* [http://www.ebi.ac.uk/msd-srv/pqs/ Protein Quaternary Structure] (PQS)

Revision as of 23:04, 25 August 2009

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

MsbA

Synonyms

fused lipid transporter subunits of ABC superfamily: membrane component[1], ATP-binding component[1], B0914[2][1], MsbA[2][1]

Product description

ATP-binding transport protein[2][3]

Lipid exporter to the outer membrane; exports LPS, phospholipids, and lipid A; drug export and resistance; ABC transporter flippase; biogenesis of outer membrane; lipid-activated ATPase[4]

EC number (for enzymes)


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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference(s) Evidence Code with/from Aspect Notes Status
GO:0005319

lipid transporter activity

PMID:11278265[5]

IMP: Inferred from Mutant Phenotype

F

Seeded from EcoCyc 12.5 [6]

complete

GO:0005524

ATP binding

PMID:8809774[7]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc 12.5 [6]

complete

GO:0005624

membrane fraction

PMID:16858726[8]

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc [9]

complete

GO:0006869

lipid transport

PMID:11278265[5]

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc 12.5 [6]

complete

GO:0015437

lipopolysaccharide-transporting ATPase activity

PMID:12119303[10]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc [9]

complete

GO:0016887

ATPase activity

PMID:12119303[10]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc 12.5 [6]

complete

GO:0000166

nucleotide binding

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR003593
SP_KW:KW-0547

F

Seeded from EcoCyc [9]

complete

GO:0005319

lipid transporter activity

GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01703

F

Seeded from EcoCyc 12.5 [6]

complete

GO:0005524

ATP binding

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR001140
InterPro:IPR003439
InterPro:IPR011527
InterPro:IPR011917
SP_KW:KW-0067

F

Seeded from EcoCyc 12.5 [6]

complete

GO:0006810

transport

GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR001140
InterPro:IPR011527
SP_KW:KW-0813

P

Seeded from EcoCyc [9]

complete

GO:0006869

lipid transport

GO_REF:0000002
GO_REF:0000004
GO_REF:0000020

IEA: Inferred from Electronic Annotation

InterPro:IPR011917
SP_KW:KW-0445
HAMAP:MF_01703

P

Seeded from EcoCyc 12.5 [6]

complete

GO:0009276

Gram-negative-bacterium-type cell wall

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011917

C

Seeded from EcoCyc [9]

complete

GO:0016020

membrane

GO_REF:0000004
GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0397
SP_KW:KW-0472

C

Seeded from EcoCyc 12.5 [6]

complete

GO:0016021

integral to membrane

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR001140
InterPro:IPR011527
InterPro:IPR011917
SP_KW:KW-0812

C

Seeded from EcoCyc [9]

complete

GO:0016787

hydrolase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0378

F

Seeded from EcoCyc [9]

complete

GO:0016887

ATPase activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003439

F

Seeded from EcoCyc 12.5 [6]

complete

GO:0017111

nucleoside-triphosphatase activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003593

F

Seeded from EcoCyc [9]

complete

GO:0042626

ATPase activity, coupled to transmembrane movement of substances

GO_REF:0000002
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001140
InterPro:IPR011527

F

Seeded from EcoCyc [9]

complete

GO:0005739

mitochondrion

PMID:16858726[8]

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc [9]

complete

GO:0005886

plasma membrane

GO_REF:0000004
GO_REF:0000004
GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_KW:KW-0997
SP_KW:KW-1003
SP_SL:SL-0037

C

Seeded from EcoCyc [9]

complete

GO:0000166

nucleotide binding

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR003593
SP_KW:KW-0547

F

Seeded from EcoCyc

complete

GO:0005319

lipid transporter activity

GO_REF:0000020
PMID:11278265[5]

IMP: Inferred from Mutant Phenotype

HAMAP:MF_01703

F

Seeded from EcoCyc

complete

GO:0005524

ATP binding

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000004
PMID:8809774[7]

IDA: Inferred from Direct Assay

InterPro:IPR001140
InterPro:IPR003439
InterPro:IPR011527
InterPro:IPR011917
InterPro:IPR017871
SP_KW:KW-0067

F

Seeded from EcoCyc

complete

GO:0005624

membrane fraction

PMID:16858726[8]

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc

complete

GO:0005886

plasma membrane

GO_REF:0000004
GO_REF:0000004
GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_KW:KW-0997
SP_KW:KW-1003
SP_SL:SL-0037

C

Seeded from EcoCyc

complete

GO:0006810

transport

GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR001140
InterPro:IPR011527
SP_KW:KW-0813

P

Seeded from EcoCyc

complete

GO:0006869

lipid transport

GO_REF:0000020
GO_REF:0000002
GO_REF:0000004
PMID:11278265[5]

IMP: Inferred from Mutant Phenotype

HAMAP:MF_01703
InterPro:IPR011917
SP_KW:KW-0445

P

Seeded from EcoCyc

complete

GO:0009276

Gram-negative-bacterium-type cell wall

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011917

C

Seeded from EcoCyc

complete

GO:0015437

lipopolysaccharide-transporting ATPase activity

PMID:12119303[10]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc

complete

GO:0016020

membrane

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0472

C

Seeded from EcoCyc

complete

GO:0016021

integral to membrane

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR001140
InterPro:IPR011527
InterPro:IPR011917
SP_KW:KW-0812

C

Seeded from EcoCyc

complete

GO:0016787

hydrolase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0378

F

Seeded from EcoCyc

complete

GO:0016887

ATPase activity

GO_REF:0000002
GO_REF:0000002
PMID:12119303[10]

IDA: Inferred from Direct Assay

InterPro:IPR003439
InterPro:IPR017871

F

Seeded from EcoCyc

complete

GO:0017111

nucleoside-triphosphatase activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003593

F

Seeded from EcoCyc

complete

GO:0042626

ATPase activity, coupled to transmembrane movement of substances

GO_REF:0000002
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001140
InterPro:IPR011527

F

Seeded from EcoCyc

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Notes

plasma membrane

C-terminus localized in the cytoplasm with 6 predicted transmembrane domains

Daley et al. (2005) [11]


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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MHNDKDLSTW QTFRRLWPTI APFKAGLIVA GVALILNAAS DTFMLSLLKP LLDDGFGKTD
RSVLVWMPLV VIGLMILRGI TSYVSSYCIS WVSGKVVMTM RRRLFGHMMG MPVSFFDKQS
TGTLLSRITY DSEQVASSSS GALITVVREG ASIIGLFIMM FYYSWQLSII LIVLAPIVSI
AIRVVSKRFR NISKNMQNTM GQVTTSAEQM LKGHKEVLIF GGQEVETKRF DKVSNRMRLQ
GMKMVSASSI SDPIIQLIAS LALAFVLYAA SFPSVMDSLT AGTITVVFSS MIALMRPLKS
LTNVNAQFQR GMAACQTLFT ILDSEQEKDE GKRVIERATG DVEFRNVTFT YPGRDVPALR
NINLKIPAGK TVALVGRSGS GKSTIASLIT RFYDIDEGEI LMDGHDLREY TLASLRNQVA
LVSQNVHLFN DTVANNIAYA RTEQYSREQI EEAARMAYAM DFINKMDNGL DTVIGENGVL
LSGGQRQRIA IARALLRDSP ILILDEATSA LDTESERAIQ AALDELQKNR TSLVIAHRLS
TIEKADEIVV VEDGVIVERG THNDLLEHRG VYAQLHKMQF GQ
Length

582

Mol. Wt

64.462 kDa

pI

8.8 (calculated)

Extinction coefficient

46,870 - 47,120 (calc based on 13 Y, 5 W, and 2 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes

Domain

342 - 578

ABC transporter

UniProt:P60752

Domain

27 - 310

ABC transmembrane type-1

UniProt:P60752

Domain

245 - 267

Predicted membrane-spanning helix.

TMD predicted by TMHMM[12]

Domain

21 - 43

Predicted membrane-spanning helix.

TMD predicted by TMHMM[12]

Domain

63 - 85

Predicted membrane-spanning helix.

TMD predicted by TMHMM[12]

Domain

140 - 162

Predicted membrane-spanning helix.

TMD predicted by TMHMM[12]

Domain

167 - 186

Predicted membrane-spanning helix.

TMD predicted by TMHMM[12]

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<motif_map/>

tmhmm.php?gene=msbA&show=pic&.jpg
Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=msbA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10613-MONOMER

UniProt (EcoliWiki Page)

UniProt:P60752

PFAM (EcoliWiki Page)

PFAM:PF00664

RefSeq (EcoliWiki Page)

RefSeq:NP_415434

ModBase (EcoliWiki Page)

ModBase:P60752


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Notes

Links

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Name URL Comments
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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 5.3 Doerrler, WT et al. (2001) An Escherichia coli mutant defective in lipid export. J. Biol. Chem. 276 11461-4 PubMed
  6. ↑ 6.0 6.1 6.2 6.3 6.4 6.5 6.6 6.7 6.8 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  7. ↑ 7.0 7.1 Polissi, A & Georgopoulos, C (1996) Mutational analysis and properties of the msbA gene of Escherichia coli, coding for an essential ABC family transporter. Mol. Microbiol. 20 1221-33 PubMed
  8. ↑ 8.0 8.1 8.2 Lasserre, JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27 3306-21 PubMed
  9. ↑ 9.00 9.01 9.02 9.03 9.04 9.05 9.06 9.07 9.08 9.09 9.10 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  10. ↑ 10.0 10.1 10.2 10.3 Doerrler, WT & Raetz, CR (2002) ATPase activity of the MsbA lipid flippase of Escherichia coli. J. Biol. Chem. 277 36697-705 PubMed
  11. ↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
  12. ↑ 12.0 12.1 12.2 12.3 12.4 Krogh, A et al. (2001) Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. J. Mol. Biol. 305 567-80 PubMed

Categories

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