PMID:10074094: Difference between revisions

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!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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[[plsB:Gene_Product(s)]]
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GO:0005887
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integral to plasma membrane
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IDA: Inferred from Direct Assay
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C
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The authors detected FLAG-tagged PlmB (expressed from a plasmid) in the membrane preparations of cells (Figure 1A).
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complete


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Revision as of 18:33, 19 October 2009

Heath, RJ and Rock, CO (1999) A missense mutation accounts for the defect in the glycerol-3-phosphate acyltransferase expressed in the plsB26 mutant.J. Bacteriol. 181:1944-6

Abstract The sn-glycerol-3-phosphate acyltransferase (plsB) catalyzes the first step in membrane phospholipid formation. A conditional Escherichia coli mutant (plsB26) has a single missense mutation (G1045A) predicting the expression of an acyltransferase with an Ala349Thr substitution. The PlsB26 protein had a significantly reduced glycerol-3-phosphate acyltransferase specific activity coupled with an elevated Km for glycerol-3-phosphate.
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Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status

plsB:Gene_Product(s)

GO:0005887

integral to plasma membrane

IDA: Inferred from Direct Assay

C

The authors detected FLAG-tagged PlmB (expressed from a plasmid) in the membrane preparations of cells (Figure 1A).

complete

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