cfa:Gene: Difference between revisions

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[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:cfa genes]]

Revision as of 00:19, 25 September 2008

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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Standard name

cfa

Mnemonic
Synonyms

ECK1657, b1661, JW1653, cdfA[1]

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Notes

Location(s) and DNA Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

37.49 minutes 

MG1655: 1739437..1740585
gbrowse_img.php?coord=1738437..1741585&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 1743127..1744275
gbrowse_img.php?coord=1742127..1745275&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

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Notes

Sequence Features

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Feature type Location Description
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Notes

Alleles and Phenotypes

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Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Δcfa (Keio:JW1653)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC9407[3]

cfa-1

CGSC:10811

cfa-2

PMID:6092874[4] PMID:3519583[5]

CGSC:17027

cfa-3

PMID:3519583[5] PMID:6092874[4]

CGSC:17030

cfa-4

PMID:3519583[5] PMID:6092874[4]

CGSC:17032

cfa-5::kan

PMID:10411742[6] PMID:3038679[7]

CGSC:64828

cfa-0

CGSC:73935

Δcfa-739::kan

PMID:16738554[2]

CGSC:100276


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Notes

Genetic Resources

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Resource Resource Type Source Notes/Reference

JW1653

Plasmid clone

Shigen

PMID:16769691[8]

Status:Clone OK

Primer 1:GCCAGTTCATCGTGTATAGAAGA

Primer 2:CCGCGAGCCACTCGAAGGCCGTT

zdd-235::Tn10

Linked marker

CAG18461 = CGSC7378[3]

est. P1 cotransduction: % [9]

Synonyms:zdc-235::Tn10

zdi-925::Tn10

Linked marker

CAG12151 = CGSC7385[3]

est. P1 cotransduction: 21% [9]

Synonyms:zdh-925::Tn10

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Notes

Accessions in Other Databases

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Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11531

EchoBASE (EcoliWiki Page)

EchoBASE:EB1493

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1661

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C2055

EcoGene (EcoliWiki Page)

EcoGene:EG11531

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:CFA

CGSC (EcoliWiki Page)

CGSC:10810

ASAP (EcoliWikiPage)

ASAP:ABE-0005555


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Notes

Links

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Name URL Comments
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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ 4.0 4.1 4.2 Grogan, DW & Cronan, JE Jr (1984) Genetic characterization of the Escherichia coli cyclopropane fatty acid (cfa) locus and neighboring loci. Mol. Gen. Genet. 196 367-72 PubMed
  5. ↑ 5.0 5.1 5.2 Grogan, DW & Cronan, JE Jr (1986) Characterization of Escherichia coli mutants completely defective in synthesis of cyclopropane fatty acids. J. Bacteriol. 166 872-7 PubMed
  6. ↑ Chang, YY & Cronan, JE Jr (1999) Membrane cyclopropane fatty acid content is a major factor in acid resistance of Escherichia coli. Mol. Microbiol. 33 249-59 PubMed
  7. ↑ Fellay, R et al. (1987) Interposon mutagenesis of soil and water bacteria: a family of DNA fragments designed for in vitro insertional mutagenesis of gram-negative bacteria. Gene 52 147-54 PubMed
  8. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  9. ↑ 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).


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