fruA:Gene: Difference between revisions

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''ECK2160, b2167, JW2154, ptsF''<ref name='LIB:Riley_2006'/>
''ECK2160, b2167, JW2154, ptsF''<ref name='LIB:Riley_2006'/>
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*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli_W3110/?plugin=FastaDumper&name=W3110:2263053..2264744&plugin_action=Go FASTA Dump]  
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!Feature type!!Location!!Description
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Synonyms:zeg-722, zei-722::Tn10  
Synonyms:zeg-722, zei-722::Tn10  
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Revision as of 08:28, 30 April 2008

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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Standard name

fruA

Mnemonic
Synonyms

ECK2160, b2167, JW2154, ptsF[1]

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Notes

Location(s) and DNA Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

48.66 minutes 

MG1655: 2259432..2257741
gbrowse_img.php?coord=2256741..2260432&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 2264744..2263053
gbrowse_img.php?coord=2262053..2265744&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Notes

Sequence Features

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Feature type Location Description
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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Notes

Alleles and Phenotypes

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Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔfruA (Keio:JW2154)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC9747[3]

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Notes

A homologous recombination using various digestive enzymes allowed the mutant allele, fruA2 to be derived.The initial transformant colonies were selected in the presence of both thiostrepton and apramycin. FruA is specifically involved in fructose catabolism. A nonpolar fruA mutation impairs growth on fructose and also leads to a constitutive low expression of the fru operon. Uptake and phosphorylation of frustose to fructose-1-phosphate by fruA is necessary to relieve repression by fruR[4].

Genetic Resources

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Resource Resource Type Source Notes/Reference

JW2154

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCAAAACGCTGCTGATTATTGA

Primer 2:CCgGCTGCTTTCGCTACTGCGTC

zbd-3104::Tn10

Linked marker

CAG12021 = CGSC7344[3]

est. P1 cotransduction: % [6]

Synonyms:zbc-3105::Tn10, zbd-3105::Tn10

napA722::Tn10

Linked marker

CAG12098 = CGSC7400[3]

est. P1 cotransduction: 15% [6]

Synonyms:zeg-722, zei-722::Tn10

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Notes

Accessions in Other Databases

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Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10336

EchoBASE (EcoliWiki Page)

EchoBASE:EB0332

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2167

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C2702

EcoGene (EcoliWiki Page)

EcoGene:EG10336

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:FRUA

CGSC (EcoliWiki Page)

CGSC:350

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Notes

Links

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Name URL Comments
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See Help:Links_table for how to enter or edit information in this section of EcoliWiki.

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ Barrière, C et al. (2005) Fructose utilization in Lactococcus lactis as a model for low-GC gram-positive bacteria: its regulator, signal, and DNA-binding site. J. Bacteriol. 187 3752-61 PubMed
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).


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