rimK:Gene: Difference between revisions

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Primer 2:CCACCACCCGTTTTCAGGCAATA  
Primer 2:CCACCACCCGTTTTCAGGCAATA  
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zbh-29::Tn10
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Linked marker
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CAG18493 = [http://cgsc2.biology.yale.edu/Strain.php?ID=39400 CGSC7353]<ref name='LIB:cgsc'/>
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est. P1 cotransduction: 1% <ref name='LIB:Nichols_linked_Tn'/>
Synonyms:zbi-29::Tn10
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zca-1230::Tn10
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Linked marker
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CAG18478 = [http://cgsc2.biology.yale.edu/Strain.php?ID=39407 CGSC7356]<ref name='LIB:cgsc'/>
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est. P1 cotransduction: 7% <ref name='LIB:Nichols_linked_Tn'/>
Synonyms:zbj-1230::Tn10
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Revision as of 21:38, 28 July 2007

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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Standard name

rimK

Mnemonic
Synonyms

ECK0843, b0852, JW0836, nek[1]

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Notes

Location(s) and DNA Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

19.21 minutes 

MG1655: 891190..892092
gbrowse_img.php?coord=890190..893092&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 892389..893291
gbrowse_img.php?coord=891389..894291&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Notes

Sequence Features

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Feature type Location Description
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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Notes

Alleles and Phenotypes

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Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability

ΔrimK (Keio:JW0836)

deletion

deletion

PMID:16738554[2]

Shigen

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Notes

Genetic Resources

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Resource Resource Type Source Notes/Reference

JW0836

Plasmid clone

Shigen

PMID:16769691[3]

Status:Clone OK

Primer 1:GCCAAAATTGCCATATTGTCCCG

Primer 2:CCACCACCCGTTTTCAGGCAATA

zbh-29::Tn10

Linked marker

CAG18493 = CGSC7353[4]

est. P1 cotransduction: 1% [5]

Synonyms:zbi-29::Tn10

zca-1230::Tn10

Linked marker

CAG18478 = CGSC7356[4]

est. P1 cotransduction: 7% [5]

Synonyms:zbj-1230::Tn10

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Notes

Accessions in Other Databases

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Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10852

EchoBASE (EcoliWiki Page)

EchoBASE:EB0845

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0852

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0985

EcoGene (EcoliWiki Page)

EcoGene:EG10852

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:RIMK

CGSC (EcoliWiki Page)

CGSC:31682

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Notes

Links

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Name URL Comments
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See Help:Links_table for how to enter or edit information in this section of EcoliWiki.

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  4. ↑ 4.0 4.1 CGSC: The Coli Genetics Stock Center
  5. ↑ 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).