ZYCY10P3S2T: Difference between revisions
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== Notes == | == Notes == | ||
{{See|Help:Strains|strains}} | |||
==Alternate Names== | |||
==Sources== | |||
SBI System Biosciences | |||
==Chromosomal Genotype== | |||
[[araE|Cp8.araE]] [[endA|ΔendA]] [[lacY|bla.lacY A177C]] [[3BAD.I-SceI]] [[2BAD.ΦC31]] [[4BAD.Φ31]] [[4BAD.Φ31]] | |||
<br> | |||
araE driven by cp8 promoter <ref name='PMID:11739756'/> <br> | |||
lacY A177C: driven by bla promoter, L-arabinose transporter function <ref name='PMID:10811905'/> <br> | |||
ΦC31: driven by L-arabinose inducible araCBAD system, intergrase | |||
==Plasmids== | |||
==Phage== | |||
==Phenotypes== | |||
==Construction== | |||
==Notes== | |||
For minicircle plasmid preparation based on attB/attP-Φ31 recombination | |||
Parental background plasmid was removed by I-SceI endonuclease | |||
BW27783 | |||
==References== | |||
{{RefHelp}} | |||
<references/> | |||
__NOTOC__ | |||
==References== | ==References== | ||
Revision as of 03:04, 2 July 2011
See Help:Strains for more information about strains in EcoliWiki.
| Name |
|---|
| Synonyms |
| Genotype |
| Phenotype |
| Plasmids |
| Phage |
| Sources |
| Construction |
| References |
| edit table |
Notes
See Help:Strains for more information about strains in EcoliWiki.
Alternate Names
Sources
SBI System Biosciences
Chromosomal Genotype
Cp8.araE ΔendA bla.lacY A177C 3BAD.I-SceI 2BAD.ΦC31 4BAD.Φ31 4BAD.Φ31
araE driven by cp8 promoter [1]
lacY A177C: driven by bla promoter, L-arabinose transporter function [2]
ΦC31: driven by L-arabinose inducible araCBAD system, intergrase
Plasmids
Phage
Phenotypes
Construction
Notes
For minicircle plasmid preparation based on attB/attP-Φ31 recombination Parental background plasmid was removed by I-SceI endonuclease
BW27783
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Khlebnikov, A et al. (2001) Homogeneous expression of the P(BAD) promoter in Escherichia coli by constitutive expression of the low-affinity high-capacity AraE transporter. Microbiology (Reading, Engl.) 147 3241-7 PubMed
- ↑ Yu, D et al. (2000) An efficient recombination system for chromosome engineering in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 97 5978-83 PubMed
References
See Help:References for how to manage references in EcoliWiki.