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!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
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micF1 (del)
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Resistant to
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lowered the resistance of wild-type bacteria to nalidixic acid, ampicillin and tetracycline
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PMID:7679383
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Revision as of 17:49, 2 May 2011

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

micF

Mnemonic

mRNA interfering cRNA

Synonyms

ECK2208, b4439, JWR0043, stc, IS113[1]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

49.81 minutes 

MG1655: 2311106..2311198
<gbrowseImage> name=NC_000913:2311106..2311198 source=ecoli preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2196911..2197003
<gbrowseImage> name=NC_012759:2196911..2197003 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2316418..2316510
<gbrowseImage> name=AC_000091:2316418..2316510 source=ecoli_W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2402094..2402186
<gbrowseImage> name=NC_010473:2402094..2402186 source=ecoli_DH10B preset=GeneLocation </gbrowseImage>


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes


Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

micF1 (del)

Resistant to

lowered the resistance of wild-type bacteria to nalidixic acid, ampicillin and tetracycline

PMID:7679383[2]



Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

Plasmid clone

Shigen

PMID:16769691[3]

Status:

Primer 1:

Primer 2:

napA722::Tn10

Linked marker

CAG12098 = CGSC7400[4]

est. P1 cotransduction: 71% [5]
Synonyms:zeg-722, zei-722::Tn10

atoS298::Tn10

Linked marker

CAG12177 gyrA+ = CGSC7525[4]

est. P1 cotransduction: 74% [5]
Synonyms:zeh-298::Tn10, zej-298::Tn10 nnnThe original CAG12177 was NalR. This is a NalS derivative constructed by S. McAteer (CGSC).


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG30063

EchoBASE (EcoliWiki Page)

EchoBASE:EB4226

EcoGene (EcoliWiki Page)

EcoGene:EG30063

CGSC (EcoliWiki Page)

CGSC:18157



Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Chou, JH et al. (1993) Posttranscriptional repression of Escherichia coli OmpF protein in response to redox stress: positive control of the micF antisense RNA by the soxRS locus. J. Bacteriol. 175 1026-31 PubMed
  3. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  4. ↑ 4.0 4.1 CGSC: The Coli Genetics Stock Center
  5. ↑ 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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