rapA:Gene: Difference between revisions

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''hepA''
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''ΔhepA769::kan''
rapADE280AA
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DE280AA
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Loss of function. Still interacts with RNAP
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PMID:16738554
seeded from [http://www.uniprot.org/uniprot/P60240 UniProt:P60240]  
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[[cgscmutation:101346|CGSC:101346]]
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rapADE280AA
''ΔhepA769::kan''
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DE280AA
 
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Loss of function. Still interacts with RNAP
 
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PMID:16738554
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[[cgscmutation:101346|CGSC:101346]]
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seeded from [http://www.uniprot.org/uniprot/P60240 UniProt:P60240]


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Revision as of 21:17, 25 February 2010

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

hepA

Mnemonic
Synonyms

ECK0060, b0059, JW0058, rapA, yabA[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

1.3 minutes 

MG1655: 63264..60358
gbrowse_img.php?coord=60358..63264&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 66068..63162
gbrowse_img.php?coord=63162..66068&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 63264..60358
gbrowse_img.php?coord=60358..63264&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 62378..60358
gbrowse_img.php?coord=60358..62378&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature type Location Description
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔhepA (Keio:JW0058)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC8366[3]

rapAK183A

K183A

Loss of function. Still interacts with RNAP

seeded from UniProt:P60240

rapADE280AA

DE280AA

Loss of function. Still interacts with RNAP

seeded from UniProt:P60240

ΔhepA769::kan

PMID:16738554[2]

CGSC:101346


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Notes

HepA has a plausible function and reasonable map location to be the gene affected by the psu-1 mutation.

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0058

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCCCTTTTACACTTGGTCAACG

Primer 2:CCCTGATGCGTTACAACGATCAA

carB96::Tn10

Linked marker

CAG12093 = CGSC7322[3]

est. P1 cotransduction: 34% [5]
Synonyms:car-96::Tn10

leuO3051::Tn10

Linked marker

CAG12095 = CGSC7324[3]

est. P1 cotransduction: 42% [5]
Synonyms:zab-3051::Tn10 nnnThe start of leuO was reannotated by Riley et al. (2006).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11083

EchoBASE (EcoliWiki Page)

EchoBASE:EB1075

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0059

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0070

EcoGene (EcoliWiki Page)

EcoGene:EG11083

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:Z0067

CGSC (EcoliWiki Page)

CGSC:29491

ASAP (EcoliWikiPage)

ASAP:ABE-0000198


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. ↑ 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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