asnS:Gene: Difference between revisions

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Fixing REL gene coord display.
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!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
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asnSP231L
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P231L
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(in temperature-sensitive mutant HO202)
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Strain variation; seeded from [http://www.uniprot.org/uniprot/P0A8M0 UniProt:P0A8M0]


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Revision as of 18:00, 18 February 2010

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

asnS

Mnemonic
Synonyms

ECK0921, b0930, JW0913, lcs, tss[1]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

21.27 minutes 

MG1655: 988208..986808
gbrowse_img.php?coord=986808..988208&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 1006076..1004676
gbrowse_img.php?coord=1004676..1006076&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 989407..988007
gbrowse_img.php?coord=988007..989407&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 1042136..1040736
gbrowse_img.php?coord=1040736..1042136&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature type Location Description


Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

asnS40(ts)

temperature sensitive

CGSC:7220

asnSP231L

P231L

(in temperature-sensitive mutant HO202)

Strain variation; seeded from UniProt:P0A8M0


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0913

Plasmid clone

Shigen

PMID:16769691[2]

Status:Clone OK

Primer 1:GCCAGCGTTGTGCCTGTAGCCGA

Primer 2:CCGAAGCTGGCGTTACGCGGAGT

zca-1230::Tn10

Linked marker

CAG18478 = CGSC7356[3]

est. P1 cotransduction: 18% [4]
Synonyms:zbj-1230::Tn10

zcb-3059::Tn10

Linked marker

CAG12094 = CGSC7358[3]

est. P1 cotransduction: 58% [4]


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10094

EchoBASE (EcoliWiki Page)

EchoBASE:EB0092

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0930

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C1072

EcoGene (EcoliWiki Page)

EcoGene:EG10094

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:ASNS

CGSC (EcoliWiki Page)

CGSC:993

ASAP (EcoliWikiPage)

ASAP:ABE-0003162



Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  3. ↑ 3.0 3.1 CGSC: The Coli Genetics Stock Center
  4. ↑ 4.0 4.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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