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{{Tab On|Quickview|pnp}}{{Tab|Gene|pnp}}{{Tab|Gene Product(s)|pnp}}{{Tab|Expression|pnp}}{{Tab|Evolution|pnp}}{{Tab|On One Page|pnp}}{{TabsBottom}}
{{Tab On|Quickview|pnp}}{{Tab|Gene|pnp}}{{Tab|Gene Product(s)|pnp}}{{Tab|Expression|pnp}}{{Tab|Evolution|pnp}}{{Tab|On One Page|pnp}}{{TabsBottom}}
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!{{table_heading_style}} |Gene Name
!align=left {{table_heading_style}} |Standard Name
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[[pnp:Gene|''pnp]]'' (synonyms: ''ECK3152, b3164, JW5851, bfl'')<ref name='LIB:Riley_2006'/>
''pnp''
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!{{table_heading_style}} |Product(s)  
!align=left {{table_heading_style}} |Gene Synonym(s)
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[[pnp:Gene_Product(s)|Pnp product]]<ref name='LIB:Riley_2006'/>: polynucleotide phosphorylase/polyadenylase product, B3164 product, Bfl product, polynucleotide phosphorylase product
''ECK3152, b3164, JW5851, bfl''<ref name='LIB:Riley_2006'/>, bfl''
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!{{table_heading_style}} |Description
!align=left {{table_heading_style}} |Product Desc.
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Pnp<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>  
Pnp<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>;


Component of [[:Category:Complex:polynucleotide phosphorylase|polynucleotide phosphorylase]]<ref name='LIB:EcoCyc11.1'/>,[[:Category:Complex:degradosome|degradosome]]<ref name='LIB:EcoCyc11.1'/>
Component of [[:Category:Complex:polynucleotide phosphorylase|polynucleotide phosphorylase]]<ref name='LIB:EcoCyc11.1'/>; [[:Category:Complex:degradosome|degradosome]]<ref name='LIB:EcoCyc11.1'/>


Polynucleotide phosphorylase; exoribonuclease; PNPase component of RNA degradosome; cold shock protein required for growth at low temperatures<ref name='LIB:EcoGene'/>
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!align=left {{table_heading_style}} |Product Synonyms(s)
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''polynucleotide phosphorylase/polyadenylase<ref name='LIB:Riley_2006'/>, B3164<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, Bfl<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, Pnp<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, polynucleotide phosphorylase<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/> , bfl, ECK3152, JW5851, b3164''
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!align=left {{table_heading_style}} |Function from GO
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<GO_nr />
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!{{table_heading_style}} |Knock-Out Phenotype
!align=left {{table_heading_style}} |Knock-Out Phenotype
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|-
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!{{table_heading_style}} |Regulation/Expression
!align=left {{table_heading_style}} |Regulation/Expression
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transcription unit(s): [[:Category:Txn_unit:rpsO-pnp|rpsO-pnp]]<ref name='LIB:EcoCyc10.6'/>, [[:Category:Txn_unit:pnp|pnp]]<ref name='LIB:EcoCyc10.6'/>, [[:Category:Txn_unit:metY-yhbC-nusA-infB-rbfA-truB-rpsO-pnp|metY-yhbC-nusA-infB-rbfA-truB-rpsO-pnp]]<ref name='LIB:EcoCyc10.6'/>  
transcription unit(s): [[:Category:Txn_unit:rpsO-pnp|rpsO-pnp]]<ref name='LIB:EcoCyc10.6'/>, [[:Category:Txn_unit:pnp|pnp]]<ref name='LIB:EcoCyc10.6'/>, [[:Category:Txn_unit:metY-yhbC-nusA-infB-rbfA-truB-rpsO-pnp|metY-yhbC-nusA-infB-rbfA-truB-rpsO-pnp]]<ref name='LIB:EcoCyc10.6'/>  
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!{{table_heading_style}} |Regulation/Activity
!align=left {{table_heading_style}} |Regulation/Activity
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!align=left {{table_heading_style}} |Quick Links
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[[File:porteco.png|link=http://porteco.org/AjaxSearch.jsp?searchString=pnp&s=search]] [[File:EcoCyc.gif|link=http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10743]] [[File:regulondb.jpg|link=http://regulondb.ccg.unam.mx/gene?term=ECK120000734&organism=ECK12&format=jsp&type=gene ]] <br/><br/> [[File:dnadisplay.png|link=http://ecoliwiki.net/tools/display_dna.php?gene=pnp&loc=MG1655%3A3309259..3307055,W3110%3A3311092..3308888,DH10B%3A3407004..3404800,REL606%3A3246471..3244267,BW2952%3A3194203..3196338]] [[File:proteindisplay.png|link=http://ecoliwiki.net/tools/display_protein.php?f=NC_000913&start=3194203&end=3196338&gene=pnp&aa=mrrrsgintsavryclrkrkditllnpivrkfqygqhtvtletgmmarqataavmvsmddtavfvtvvgqkkakpgqdffpltvnyqertyaagripgsffrregrpsegetliarlidrpirplfpegfvnevqviatvvsvnpqvnpdivamigasaalslsgipfngpigaarvgyindqyvlnptqdelkeskldlvvagteaavlmveseaqllsedqmlgavvfgheqqqvviqninelvkeagkprwdwqpepvnealnarvaalaearlsdayritdkqeryaqvdviksetiatllaedetldenelgeilhaieknvvrsrvlagepridgrekdmirgldvrtgvlprthgsalftrgetqalvtatlgtardaqvldelmgertdtflfhynfppysvgetgmvgspkrreighgrlakrgvlavmpdmdkfpytvrvvseitesngsssmasvcgaslalmdagvpikaavagiamglvkegdnyvvlsdilgdedhlgdmdfkvagsrdgisalqmdikiegitkeimqvalnqakgarlhilgvmeqainaprgdisefaprihtikinpdkikdvigkggsviralteetgttieieddgtvkiaatdgekakhairrieeitaeievgrvytgkvtrivdfgafvaigggkeglvhisqiadkrvekvtdylqmgqevpvkvlevdrqgrirlsikeateqsqpaaapeapaaeqge]]&nbsp;&nbsp;  [[File:pubmed.jpg|link=http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=Search&Term=pnp+AND+escherichia+coli]]&nbsp;&nbsp;  [[File:textpresso.jpg|link=http://textpresso.tamu.edu/cgi-bin/textpresso/search?q=pnp]]&nbsp;&nbsp;
 
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==Notes==
===Notes===
{{BacktoTop}}
Implicated in degadation of sRNAs<ref name='PMID:18203924'/>
 
Cold induction occurs by reversal of post-translational autoregulation. The pnp mRNA leader is cleaved by RNase III, allowing PNPase to repress its own translation. Mutation of Gly454 impairs autogenous regulation without affecting catalytic activity. Mutants have elevated OM vesiculation (McBroom, 2006). Pnp can add long polynucleotide tails to mRNAs terminated by Rho factor (Mohanty, 2006). The overexpression of the Pnp S1 domain suppresses the cold growth defect of quadruple cspABEG mutant strain BX04 (Xia, 2001).<ref name='LIB:EcoGene'/>




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==References==
==References==
{{BacktoTop}}
<references/>
<references/>
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Latest revision as of 21:54, 27 May 2013

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
References Suggestions
Standard Name

pnp

Gene Synonym(s)

ECK3152, b3164, JW5851, bfl[1], bfl

Product Desc.

Pnp[2][3];

Component of polynucleotide phosphorylase[3]; degradosome[3]

Polynucleotide phosphorylase; exoribonuclease; PNPase component of RNA degradosome; cold shock protein required for growth at low temperatures[4]

Product Synonyms(s)

polynucleotide phosphorylase/polyadenylase[1], B3164[2][1], Bfl[2][1], Pnp[2][1], polynucleotide phosphorylase[2][1] , bfl, ECK3152, JW5851, b3164

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): rpsO-pnp[2], pnp[2], metY-yhbC-nusA-infB-rbfA-truB-rpsO-pnp[2]

Regulation/Activity
Quick Links



        

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Notes

[back to top]


Implicated in degadation of sRNAs[5]

Cold induction occurs by reversal of post-translational autoregulation. The pnp mRNA leader is cleaved by RNase III, allowing PNPase to repress its own translation. Mutation of Gly454 impairs autogenous regulation without affecting catalytic activity. Mutants have elevated OM vesiculation (McBroom, 2006). Pnp can add long polynucleotide tails to mRNAs terminated by Rho factor (Mohanty, 2006). The overexpression of the Pnp S1 domain suppresses the cold growth defect of quadruple cspABEG mutant strain BX04 (Xia, 2001).[4]



References

[back to top]


  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ Andrade, JM & Arraiano, CM (2008) PNPase is a key player in the regulation of small RNAs that control the expression of outer membrane proteins. RNA 14 543-51 PubMed
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