Cis Element:Phage lambda ori: Difference between revisions

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__NOTOC__
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==Cis element summary table==
==Cis element summary table==
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|-
!align=left {{table_heading_style}} |Standard Name
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''Lambda ori''
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!align=left {{table_heading_style}} |Synonyms
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|-
!align=left {{table_heading_style}} |Function
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Initiation control site for replication of the lambda genome
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!align=left {{table_heading_style}} |Location
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within ''O'' gene
|-
!align=left {{table_heading_style}} |SO terms
||
 
 
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===Notes===
===Notes===


== Location and Sequence ==
== Location and Sequence ==
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!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-
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Phage Lambda (NC_001416)
|
 
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NC_001416: 39034..39137<br />{{GBrowseFigureByCoord|39024|39147|bacteriophage_lambda|NC_001416}}
 
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{{GBrowseLink|bacteriophage_lambda|NC_001416|39034|39137|Gbrowse (Bacteriophage lambda)}}
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{{GBrowseFastaLink|bacteriophage_lambda|NC_001416|39034|39137|FASTA Dump}}
 
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===Notes===
===Notes===
* The lambda ''ori'' has 4 repeated segments (commonly referred to as 'iterons') located at the following coordinates<ref name='PMID:6455332'/>:
#* ori1 - nt 39034 - 39051
#* ori2 - nt 39054 - 39071
#* ori3 - nt 39078 - 39095
#* ori4 - nt 39101 - 39118
Below is the location of each of the ''ori'' iteron (underlined) in the lambda genomic sequence <ref name='PMID:6455332'/> :
<span style="font-size:larger;"><b>Lambda  nt39034  <u>ATCCCTCAAA ACGAGGGA</u>AA <u>ATCCCCTAAAA CGAGGGAT</u>AA AAC<u>ATCCCTC AAATTGGGGG ATTGCT</u>  39100
Lambda  nt39101  <u>ATCCCTCAAA ACAGGGGG</u>AC ACAAAAGACA CTATTACAAA AGAAAAAAGA AAA  39153 </b></span>


== Interactions==
== Interactions==
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!|Partner Type!!Partner!!Notes!!References!!Evidence
|-
|
Protein
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Phage lambda O protein
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|
PMID:6264392
|
Purified O protein protects lambda DNA in the ''ori'' region from ExoIII nuclease digestion (FIgure 2) and DNase cleavage (Figure 4).
 
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===Notes===
===Notes===
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== Mutant alleles and phenotypes ==
== Mutant alleles and phenotypes ==
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!|Allele!!Nt change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
 
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===Notes===
===Notes===
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== Genetic Resources ==
== Genetic Resources ==
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!|Resource!!Resource Type!!Source!!Notes/Reference
 
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===Notes===
===Notes===


== Accessions ==
== Accessions ==
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!|Database!!Accession!!Notes
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[[EcoCyc]]
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EcoCyc:
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[[EcoGene]]
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EcoGene:
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[[RegulonDB]]
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RegulonDB:
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
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[[EchoBASE]]
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EchoBASE:
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[[ASAP]]
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ASAP:
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
 
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===Notes===
===Notes===
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<references/>
<references />


===Categories===
===Categories===
[[Category:Phage lambda:Cis_Element]]

Latest revision as of 08:40, 27 May 2013

Cis element summary table

Standard Name

Lambda ori

Synonyms
Function

Initiation control site for replication of the lambda genome

Location

within O gene

SO terms


Notes

Location and Sequence

[back to top]


See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

Phage Lambda (NC_001416)

NC_001416: 39034..39137
gbrowse_img.php?coord=39024..39147&genome=bacteriophage_lambda&name=NC_001416&trackset=0&width=300&.png


Notes

  • The lambda ori has 4 repeated segments (commonly referred to as 'iterons') located at the following coordinates[1]:
    • ori1 - nt 39034 - 39051
    • ori2 - nt 39054 - 39071
    • ori3 - nt 39078 - 39095
    • ori4 - nt 39101 - 39118

Below is the location of each of the ori iteron (underlined) in the lambda genomic sequence [1] :

Lambda  nt39034  ATCCCTCAAA ACGAGGGAAA ATCCCCTAAAA CGAGGGATAA AACATCCCTC AAATTGGGGG ATTGCT  39100
Lambda  nt39101  ATCCCTCAAA ACAGGGGGAC ACAAAAGACA CTATTACAAA AGAAAAAAGA AAA   39153 

Interactions

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See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Phage lambda O protein

PMID:6264392[2]

Purified O protein protects lambda DNA in the ori region from ExoIII nuclease digestion (FIgure 2) and DNase cleavage (Figure 4).


Notes

Mutant alleles and phenotypes

[back to top]


Allele Nt change(s) Phenotype: Type Phenotype: Description Reference Availability Comments


Notes

Genetic Resources

[back to top]


See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

Notes

Accessions

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:

Escherichia coli str. K-12 substr. MG1655


Notes

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 Moore, DD et al. () Sequence organization of the origins of DNA replication in lambdoid coliphages. Gene 14 91-101 PubMed
  2. ↑ Tsurimoto, T & Matsubara, K (1981) Purified bacteriophage lambda O protein binds to four repeating sequences at the lambda replication origin. Nucleic Acids Res. 9 1789-99 PubMed

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