Cis Element:Phage lambda ori: Difference between revisions
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==Cis element summary table== | ==Cis element summary table== | ||
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!align=left {{table_heading_style}} |Standard Name | |||
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''Lambda ori'' | |||
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!align=left {{table_heading_style}} |Synonyms | |||
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!align=left {{table_heading_style}} |Function | |||
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Initiation control site for replication of the lambda genome | |||
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!align=left {{table_heading_style}} |Location | |||
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within ''O'' gene | |||
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!align=left {{table_heading_style}} |SO terms | |||
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===Notes=== | ===Notes=== | ||
== Location and Sequence == | == Location and Sequence == | ||
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!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links | |||
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Phage Lambda (NC_001416) | |||
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NC_001416: 39034..39137<br />{{GBrowseFigureByCoord|39024|39147|bacteriophage_lambda|NC_001416}} | |||
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{{GBrowseLink|bacteriophage_lambda|NC_001416|39034|39137|Gbrowse (Bacteriophage lambda)}} | |||
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{{GBrowseFastaLink|bacteriophage_lambda|NC_001416|39034|39137|FASTA Dump}} | |||
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===Notes=== | ===Notes=== | ||
* The lambda ''ori'' has 4 repeated segments (commonly referred to as 'iterons') located at the following coordinates<ref name='PMID:6455332'/>: | |||
#* ori1 - nt 39034 - 39051 | |||
#* ori2 - nt 39054 - 39071 | |||
#* ori3 - nt 39078 - 39095 | |||
#* ori4 - nt 39101 - 39118 | |||
Below is the location of each of the ''ori'' iteron (underlined) in the lambda genomic sequence <ref name='PMID:6455332'/> : | |||
<span style="font-size:larger;"><b>Lambda nt39034 <u>ATCCCTCAAA ACGAGGGA</u>AA <u>ATCCCCTAAAA CGAGGGAT</u>AA AAC<u>ATCCCTC AAATTGGGGG ATTGCT</u> 39100 | |||
Lambda nt39101 <u>ATCCCTCAAA ACAGGGGG</u>AC ACAAAAGACA CTATTACAAA AGAAAAAAGA AAA 39153 </b></span> | |||
== Interactions== | == Interactions== | ||
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!|Partner Type!!Partner!!Notes!!References!!Evidence | |||
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Protein | |||
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Phage lambda O protein | |||
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PMID:6264392 | |||
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Purified O protein protects lambda DNA in the ''ori'' region from ExoIII nuclease digestion (FIgure 2) and DNase cleavage (Figure 4). | |||
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===Notes=== | ===Notes=== | ||
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== Mutant alleles and phenotypes == | == Mutant alleles and phenotypes == | ||
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!|Allele!!Nt change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments | |||
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===Notes=== | ===Notes=== | ||
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== Genetic Resources == | == Genetic Resources == | ||
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!|Resource!!Resource Type!!Source!!Notes/Reference | |||
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===Notes=== | ===Notes=== | ||
== Accessions == | == Accessions == | ||
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!|Database!!Accession!!Notes | |||
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[[EcoCyc]] | |||
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EcoCyc: | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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[[EcoGene]] | |||
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EcoGene: | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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[[RegulonDB]] | |||
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RegulonDB: | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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[[EchoBASE]] | |||
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EchoBASE: | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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[[ASAP]] | |||
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ASAP: | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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===Notes=== | ===Notes=== | ||
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{{BacktoTop}} | {{BacktoTop}} | ||
{{RefHelp}} | {{RefHelp}} | ||
<references/> | <references /> | ||
===Categories=== | ===Categories=== | ||
[[Category:Phage lambda:Cis_Element]] | |||
Latest revision as of 08:40, 27 May 2013
Cis element summary table
| Standard Name |
Lambda ori |
|---|---|
| Synonyms | |
| Function |
Initiation control site for replication of the lambda genome |
| Location |
within O gene |
| SO terms |
|
| edit table |
Notes
Location and Sequence
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
Phage Lambda (NC_001416) |
NC_001416: 39034..39137 |
|||
| edit table |
Notes
- The lambda ori has 4 repeated segments (commonly referred to as 'iterons') located at the following coordinates[1]:
- ori1 - nt 39034 - 39051
- ori2 - nt 39054 - 39071
- ori3 - nt 39078 - 39095
- ori4 - nt 39101 - 39118
Below is the location of each of the ori iteron (underlined) in the lambda genomic sequence [1] :
Lambda nt39034 ATCCCTCAAA ACGAGGGAAA ATCCCCTAAAA CGAGGGATAA AACATCCCTC AAATTGGGGG ATTGCT 39100
Lambda nt39101 ATCCCTCAAA ACAGGGGGAC ACAAAAGACA CTATTACAAA AGAAAAAAGA AAA 39153
Interactions
See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
|
Protein |
Phage lambda O protein |
Purified O protein protects lambda DNA in the ori region from ExoIII nuclease digestion (FIgure 2) and DNase cleavage (Figure 4). | ||
| edit table |
Notes
Mutant alleles and phenotypes
| Allele | Nt change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|
| edit table |
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
| edit table |
Notes
Accessions
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
EcoCyc: |
Escherichia coli str. K-12 substr. MG1655 | |
|
EcoGene: |
Escherichia coli str. K-12 substr. MG1655 | |
|
RegulonDB: |
Escherichia coli str. K-12 substr. MG1655 | |
|
EchoBASE: |
Escherichia coli str. K-12 substr. MG1655 | |
|
ASAP: |
Escherichia coli str. K-12 substr. MG1655 | |
| edit table |
Notes
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 Moore, DD et al. () Sequence organization of the origins of DNA replication in lambdoid coliphages. Gene 14 91-101 PubMed
- ↑ Tsurimoto, T & Matsubara, K (1981) Purified bacteriophage lambda O protein binds to four repeating sequences at the lambda replication origin. Nucleic Acids Res. 9 1789-99 PubMed