Cis Element:Phage lambda ori

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Cis element summary table

Standard Name

Lambda ori

Synonyms
Function

Initiation control site for replication of the lambda genome

Location

within O gene

SO terms


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Notes

Location and Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

Phage Lambda (NC_001416)

NC_001416: 39034..39137
gbrowse_img.php?coord=39024..39147&genome=bacteriophage_lambda&name=NC_001416&trackset=0&width=300&.png

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Notes

  • The lambda ori has 4 repeated segments (commonly referred to as 'iterons') located at the following coordinates[1]:
    • ori1 - nt 39034 - 39051
    • ori2 - nt 39054 - 39071
    • ori3 - nt 39078 - 39095
    • ori4 - nt 39101 - 39118

Below is the location of each of the ori iteron (underlined) in the lambda genomic sequence [1] : 

Lambda  nt39034  ATCCCTCAAA ACGAGGGAAA ATCCCCTAAAA CGAGGGATAA AACATCCCTC AAATTGGGGG ATTGCT  39100
Lambda  nt39101  ATCCCTCAAA ACAGGGGGAC ACAAAAGACA CTATTACAAA AGAAAAAAGA AAA   39153

Interactions

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Partner Type Partner Notes References Evidence

Protein

Phage lambda O protein

PMID:6264392[2]

Purified O protein protects lambda DNA in the ori region from ExoIII nuclease digestion (FIgure 2) and DNase cleavage (Figure 4).

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Notes

Mutant alleles and phenotypes

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Allele Nt change(s) Phenotype: Type Phenotype: Description Reference Availability Comments
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Notes

Genetic Resources

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Resource Resource Type Source Notes/Reference
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Notes

Accessions

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Database Accession Notes

EcoCyc

EcoCyc:

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:

Escherichia coli str. K-12 substr. MG1655

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Notes

References

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  1. ↑ 1.0 1.1 Moore, DD et al. () Sequence organization of the origins of DNA replication in lambdoid coliphages. Gene 14 91-101 PubMed
  2. ↑ Tsurimoto, T & Matsubara, K (1981) Purified bacteriophage lambda O protein binds to four repeating sequences at the lambda replication origin. Nucleic Acids Res. 9 1789-99 PubMed

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