bcsE:Expression: Difference between revisions
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{{Tab|Quickview|bcsE}}{{Tab|Gene|bcsE}}{{Tab|Gene Product(s)|bcsE}}{{Tab On|Expression|bcsE}}{{Tab|Evolution|bcsE}}{{Tab|On One Page|bcsE}}{{TabsBottom}} | {{Tab|Quickview|bcsE}}{{Tab|Gene|bcsE}}{{Tab|Gene Product(s)|bcsE}}{{Tab On|Expression|bcsE}}{{Tab|Evolution|bcsE}}{{Tab|On One Page|bcsE}}{{TabsBottom}} | ||
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|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]] | |{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]] | ||
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==Overview== | ===Overview=== | ||
{{Expression_overview_placeholder}} | {{Expression_overview_placeholder}} | ||
==Cellular Levels== | |||
{{BacktoTop}} | |||
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!|Molecule!!Organism or Strain!!Value!!Units!!Experimental Conditions!!Assay used!!Notes!!Reference(s) | |||
|- | |||
| | |||
Protein | |||
| | |||
Ecoli K-12 | |||
| | |||
0.564+/-0.683 | |||
| | |||
Molecules/cell | |||
| | |||
*Medium: | |||
*Temperature (°C): | |||
*Doubling time (min): Growth Rate(min): * | |||
*Growth phase: other: * | |||
| | |||
| | |||
Single Molecule Fluorescence | |||
| | |||
PMID:20671182 | |||
|- | |||
| | |||
Protein | |||
| | |||
''E. coli'' K-12 MG1655 | |||
| | |||
332 | |||
| | |||
molecules/cell/generation | |||
| | |||
*Medium: MOPS Complete | |||
* Temperature (°C): 37 | |||
*Doubling time (min): | |||
* *Growth phase: | |||
*OD: | |||
*other: | |||
| | |||
Ribosome Profiling | |||
| | |||
| | |||
PMID: 24766808 | |||
|- | |||
| | |||
Protein | |||
| | |||
''E. coli'' K-12 MG1655 | |||
| | |||
230 | |||
| | |||
molecules/cell/generation | |||
| | |||
*Medium: MOPS Minimal | |||
* Temperature (°C): 37 | |||
*Doubling time (min): | |||
* *Growth phase: | |||
*OD: | |||
*other: | |||
| | |||
Ribosome Profiling | |||
| | |||
| | |||
PMID: 24766808 | |||
|- | |||
| | |||
Protein | |||
| | |||
''E. coli'' K-12 MG1655 | |||
| | |||
310 | |||
| | |||
molecules/cell/generation | |||
| | |||
*Medium: MOPS Complete without Methionine | |||
* Temperature (°C): 37 | |||
*Doubling time (min): | |||
* *Growth phase: | |||
*OD: | |||
*other: | |||
| | |||
Ribosome Profiling | |||
| | |||
| | |||
PMID: 24766808 | |||
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===Notes=== | |||
==Transcription and Transcriptional Regulation== | ==Transcription and Transcriptional Regulation== | ||
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{|{{Prettytable}} | {|{{Prettytable}} | ||
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!Transcription unit(s) | !|Transcription unit(s) | ||
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[[:Category:Txn_unit:bcsE|bcsE]] | [[:Category:Txn_unit:bcsE|bcsE]] | ||
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<!--Transcription_notes--></protect> | <!--Transcription_notes--></protect> | ||
===Notes=== | ===Notes=== | ||
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==Translation and Regulation of Translation== | ==Translation and Regulation of Translation== | ||
<protect> | {{BacktoTop}} | ||
{{ | <protect><gbrowseImage> | ||
name=NC_000913:3694461..3694501 | |||
source=MG1655 | |||
type=Gene+DNA_+Protein | |||
preset=Nterminus | |||
</gbrowseImage> | |||
{{Nterm_closeup}} | |||
<!--Translation_notes--></protect> | <!--Translation_notes--></protect> | ||
===Notes=== | |||
{{Expression_translation_placeholder}} | {{Expression_translation_placeholder}} | ||
==Turnover and Regulation of Turnover== | ==Turnover and Regulation of Turnover== | ||
<!--Turnover_notes--></protect> | |||
===Notes=== | |||
{{Expression_turnover_placeholder}} | {{Expression_turnover_placeholder}} | ||
==Experimental== | ==Experimental== | ||
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<protect> | <protect> | ||
<big>'''Mutations Affecting Expression'''</big> | |||
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<!--box uid=421aa90e079fa326b6494f812ad13e79.. | <big>'''Expression Studies'''</big> | ||
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!Type!!Reference!!Notes | !|Type!!Reference!!Notes | ||
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microarray | |||
| | |||
[[geoprofiles:b3536|GEO Profiles:b3536]] ([[Gene_Expression_Omnibus_%28GEO%29|EcoliWiki Page]]) | |||
| | |||
NCBI GEO profiles for ''bcsE'' | |||
|- | |||
| | |||
microarray | |||
| | |||
[http://genexpdb.ou.edu/index.php?query=b3536 GenExpDB:b3536] ([[EcoliGenExpDB|EcoliWiki Page]]) | |||
| | |||
Summary of data for bcsE from multiple microarray studies | |||
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<!--box uid=421aa90e079fa326b6494f812ad13e79.. | <big>'''Expression Resources'''</big> | ||
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!Resource Name!!Resource Type!!Description!!Source!!Notes | !|Resource Name!!Resource Type!!Description!!Source!!Notes | ||
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GFP Fusion | |||
| | |||
Intergenic region (3693732..3694091) fused to <i>gfpmut2</i>. | |||
| | |||
[http://www.openbiosystems.com/GeneExpression/Non-Mammalian/Bacteria/EcoliPromoters/ OpenBioSystems][[Category:Genes in OpenBioSystems with Promoter Fusions]] | |||
| | |||
GFP fusion described in Zaslaver, <i>et al.</i><br />Plate:109-AZ18; Well:D1<ref name="PMID: 16862137" /> | |||
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<protect><!--Expression_experimental_notes--></protect> | <protect><!--Expression_experimental_notes--></protect> | ||
===Notes=== | ===Notes=== | ||
==Accessions Related to | ==Accessions Related to ''bcsE'' Expression== | ||
<!--box uid=421aa90e079fa326b6494f812ad13e79.. | {{BacktoTop}} | ||
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|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!Database!!Accession!!Notes | !|Database!!Accession!!Notes | ||
|- | |- | ||
| | | | ||
[[EcoCyc]] | |||
| | | | ||
EcoCyc:EG12263 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
| | | | ||
[[EchoBASE]] | |||
| | | | ||
EchoBASE:EB2172 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
| | | | ||
[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]]) | [http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]]) | ||
| | | | ||
[http://chase.ou.edu/macro/searchlink.php?bnum=b3536 EcoliGenExpDB:b3536] | [http://chase.ou.edu/macro/searchlink.php?bnum=b3536 EcoliGenExpDB:b3536] | ||
| | | | ||
|- | |- | ||
| | |||
[[EcoGene]] | |||
| | |||
EcoGene:EG12263 | |||
| | |||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |||
| | |||
[[RegulonDB]] | |||
| | |||
RegulonDB:ECK120002144 | |||
| | |||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |||
| | | | ||
[[ASAP]] | |||
| | | | ||
ASAP:ABE-0011554 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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<protect><!--Accessions_notes--></protect> | <protect><!--Accessions_notes--></protect> | ||
===Notes=== | ===Notes=== | ||
==Links== | ==Links== | ||
{{BacktoTop}} | |||
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==References== | ==References== | ||
{{BacktoTop}} | |||
{{RefHelp}} | {{RefHelp}} | ||
<references/> | <references /> | ||
__NOTOC__ | __NOTOC__ | ||
===Categories=== | |||
{{BacktoTop}} | |||
[[Category:Txn_unit:bcsE]] | |||
Latest revision as of 19:51, 21 July 2015
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Overview | Transcription | Translation | Turnover | Experimental | Links | References | Suggestions |
Overview
This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Cellular Levels
| Molecule | Organism or Strain | Value | Units | Experimental Conditions | Assay used | Notes | Reference(s) |
|---|---|---|---|---|---|---|---|
|
Protein |
Ecoli K-12 |
0.564+/-0.683 |
Molecules/cell |
|
Single Molecule Fluorescence |
||
|
Protein |
E. coli K-12 MG1655 |
332 |
molecules/cell/generation |
|
Ribosome Profiling |
||
|
Protein |
E. coli K-12 MG1655 |
230 |
molecules/cell/generation |
|
Ribosome Profiling |
||
|
Protein |
E. coli K-12 MG1655 |
310 |
molecules/cell/generation |
|
Ribosome Profiling |
||
| edit table |
Notes
Transcription and Transcriptional Regulation
|
See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.
|
Notes
This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Translation and Regulation of Translation
<gbrowseImage> name=NC_000913:3694461..3694501 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.
Notes
This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Turnover and Regulation of Turnover
</protect>
Notes
This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.
Experimental
Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.
| Allele Name | Mutation | Phenotype | Reference |
|---|---|---|---|
| edit table |
Expression Studies
See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.
| Type | Reference | Notes |
|---|---|---|
|
microarray |
NCBI GEO profiles for bcsE | |
|
microarray |
Summary of data for bcsE from multiple microarray studies | |
| edit table |
Expression Resources
See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.
| Resource Name | Resource Type | Description | Source | Notes |
|---|---|---|---|---|
|
GFP Fusion |
Intergenic region (3693732..3694091) fused to gfpmut2. |
GFP fusion described in Zaslaver, et al. | ||
| edit table |
<protect>
Notes
Accessions Related to bcsE Expression
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Taniguchi, Y et al. (2010) Quantifying E. coli proteome and transcriptome with single-molecule sensitivity in single cells. Science 329 533-8 PubMed
- ↑ 2.0 2.1 2.2 Li, GW et al. (2014) Quantifying absolute protein synthesis rates reveals principles underlying allocation of cellular resources. Cell 157 624-35 PubMed
- ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed
Categories
