ftsL:Expression: Difference between revisions

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<noinclude>{{TabsTop}}
<noinclude>{{TabsTop}}
{{Tab|Quickview|ftsL}}{{Tab|Gene|ftsL}}{{Tab|Gene Product(s)|ftsL}}{{Tab On|Expression|ftsL}}{{Tab|Evolution|ftsL}}{{Tab|On One Page|ftsL}}{{TabsBottom}}
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|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
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</noinclude>[[Category:Txn_unit:mraZW-ftsLI-murEF-mraY-murD-ftsW-murGC-ddlB-ftsQAZ]]
</noinclude>
==Overview==
===Overview===
{{Expression_overview_placeholder}}
{{Expression_overview_placeholder}}
==Cellular Levels==
{{BacktoTop}}
<!--box uid=bc145ea147f7e36caf09c1bfeed4ee9d.32700.W49c026fa03d55-->
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="W49c026fa03d55"  class=" tableEdit MoleculesPerCellTable" 
|- align='left' bgcolor='#CCCCFF'
!|Molecule!!Organism or Strain!!Value!!Units!!Experimental Conditions!!Assay used!!Notes!!Reference(s)
|-
|
Protein
|
E. coli LMG83, LMG145, LMG142 (see table 1)
|
20 - 40
|
molecules/cell
|
*Medium:  NZY (rich media, recipe described on page 7717)
*Temperature (°C):  37
*Doubling time (min): Growth Rate(min): 
*Growth phase: other:
|
enzyme activity
|
A chromosomal FtsL-phoA (alkaline phosphatase) fusion was assayed for AP activity & shown to be relatively comparable to the previously reported activity/concentration of FtsQ-phoA estimated at 20-40 molecules per cell (reported by Carson et al., PMID:2007547).
|
PMID:1332942
|-
|
Protein
|
''E. coli'' K-12 MG1655
|
416
|
molecules/cell/generation
|
*Medium: MOPS Complete 
* Temperature (°C):  37
*Doubling time (min):
* *Growth phase: 
*OD:
*other:
|
Ribosome Profiling
|
|
PMID: 24766808
|-
|
Protein
|
''E. coli'' K-12 MG1655
|
201
|
molecules/cell/generation
|
*Medium: MOPS Minimal 
* Temperature (°C):  37
*Doubling time (min):
* *Growth phase: 
*OD:
*other:
|
Ribosome Profiling
|
|
PMID: 24766808
|-
|
Protein
|
''E. coli'' K-12 MG1655
|
423
|
molecules/cell/generation
|
*Medium: MOPS Complete without Methionine 
* Temperature (°C):  37
*Doubling time (min):
* *Growth phase: 
*OD:
*other:
|
Ribosome Profiling
|
|
PMID: 24766808
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===Notes===
==Transcription and Transcriptional Regulation==
==Transcription and Transcriptional Regulation==
{{BacktoTop}}
<protect>
<protect>
{|{{Prettytable}}
{|{{Prettytable}}
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!Transcription unit(s)
!|Transcription unit(s)
|-
|-  
|
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[[:Category:Txn_unit:mraZW-ftsLI-murEF-mraY-murD-ftsW-murGC-ddlB-ftsQAZ|mraZW-ftsLI-murEF-mraY-murD-ftsW-murGC-ddlB-ftsQAZ]]  
[[:Category:Txn_unit:mraZW-ftsLI-murEF-mraY-murD-ftsW-murGC-ddlB-ftsQAZ|mraZW-ftsLI-murEF-mraY-murD-ftsW-murGC-ddlB-ftsQAZ]]  
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<!--Transcription_notes--></protect>
<!--Transcription_notes--></protect>
===Notes===
===Notes===
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==Translation and Regulation of Translation==
==Translation and Regulation of Translation==
<protect>{{GBrowseFigureByCoord|91017|91047|ecoli|MG1655|GenesNew+DNA}}
{{BacktoTop}}
{{Expression_translation_help}}
<protect><gbrowseImage>
name=NC_000913:91012..91052
source=MG1655
type=Gene+DNA_+Protein
preset=Nterminus
</gbrowseImage>
{{Nterm_closeup}}
 
<!--Translation_notes--></protect>
<!--Translation_notes--></protect>
===Notes===
{{Expression_translation_placeholder}}
{{Expression_translation_placeholder}}


==Turnover and Regulation of Turnover==
==Turnover and Regulation of Turnover==
<!--Turnover_notes--></protect>
===Notes===
{{Expression_turnover_placeholder}}
{{Expression_turnover_placeholder}}


==Experimental==
==Experimental==
{{BacktoTop}}
<protect>
<protect>
===Mutations Affecting Expression===
<big>'''Mutations Affecting Expression'''</big>
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===Expression Studies===
 
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<big>'''Expression Studies'''</big>
<!--*** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. *** -->
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|
microarray
|
[[geoprofiles:b0083|GEO Profiles:b0083]] ([[Gene_Expression_Omnibus_%28GEO%29|EcoliWiki Page]])
|
NCBI GEO profiles for ''ftsL''
|-
|
microarray
|
[http://genexpdb.ou.edu/index.php?query=b0083 GenExpDB:b0083] ([[EcoliGenExpDB|EcoliWiki Page]])
|
Summary of data for ftsL from multiple microarray studies 
 
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===Expression Resources===
 
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<big>'''Expression Resources'''</big>
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<protect><!--Expression_experimental_notes--></protect>
<protect><!--Expression_experimental_notes--></protect>
===Notes===
===Notes===




==Accessions Related to ftsL Expression==
==Accessions Related to ''ftsL'' Expression==
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|- {{table_heading_style}}
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!Database!!Accession!!Notes
!|Database!!Accession!!Notes
|-
|-  
|
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
|
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG11086 EcoCyc:EG11086] 
EcoCyc:EG11086
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|-  
|
|
[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
[[EchoBASE]]
|
|
[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB1078 EchoBASE:EB1078] 
EchoBASE:EB1078
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|-  
|
|
[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])  
[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])  
|
|
[http://chase.ou.edu/macro/searchlink.php?bnum=b0083 EcoliGenExpDB:b0083]
[http://chase.ou.edu/macro/searchlink.php?bnum=b0083 EcoliGenExpDB:b0083]
|
|
   
   
|-
|-
|
[[EcoGene]]
|
EcoGene:EG11086
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[[RegulonDB]]
|
RegulonDB:ECK120001074
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|
|
[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
[[ASAP]]
|
|
[http://ecogene.org/geneinfo.php?eg_id=EG11086 EcoGene:EG11086] 
ASAP:ABE-0000306
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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<protect><!--Accessions_notes--></protect>
<protect><!--Accessions_notes--></protect>
===Notes===
===Notes===
{{EXPRESSION_ACCESSIONS_NOTES}}
 


==Links==
==Links==
{{BacktoTop}}
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<noinclude>
<noinclude>
==References==
==References==
{{BacktoTop}}
{{RefHelp}}
{{RefHelp}}
<references/>
<references />
__NOTOC__
__NOTOC__
</noinclude>
===Categories===
{{BacktoTop}}
[[Category:Txn_unit:mraZW-ftsLI-murEF-mraY-murD-ftsW-murGC-ddlB-ftsQAZ]]

Latest revision as of 19:37, 21 July 2015

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Overview Transcription Translation Turnover Experimental Links References Suggestions

Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

[back to top]


See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli LMG83, LMG145, LMG142 (see table 1)

20 - 40

molecules/cell

  • Medium: NZY (rich media, recipe described on page 7717)
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

enzyme activity

A chromosomal FtsL-phoA (alkaline phosphatase) fusion was assayed for AP activity & shown to be relatively comparable to the previously reported activity/concentration of FtsQ-phoA estimated at 20-40 molecules per cell (reported by Carson et al., PMID:2007547[1]).

PMID:1332942[2]

Protein

E. coli K-12 MG1655

416

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID:24766808[3]

Protein

E. coli K-12 MG1655

201

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID:24766808[3]

Protein

E. coli K-12 MG1655

423

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID:24766808[3]

edit table

Notes

Transcription and Transcriptional Regulation

[back to top]


See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

mraZW-ftsLI-murEF-mraY-murD-ftsW-murGC-ddlB-ftsQAZ

edit table
Figure courtesy of RegulonDB


Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

[back to top]


<gbrowseImage> name=NC_000913:91012..91052 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.


Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

[back to top]


Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
edit table


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b0083 (EcoliWiki Page)

NCBI GEO profiles for ftsL

microarray

GenExpDB:b0083 (EcoliWiki Page)

Summary of data for ftsL from multiple microarray studies

edit table


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
edit table

<protect>

Notes

Accessions Related to ftsL Expression

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11086

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1078

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0083

EcoGene

EcoGene:EG11086

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001074

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000306

Escherichia coli str. K-12 substr. MG1655

edit table


Notes

Links

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References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. ↑ Carson, MJ et al. (1991) The FtsQ protein of Escherichia coli: membrane topology, abundance, and cell division phenotypes due to overproduction and insertion mutations. J. Bacteriol. 173 2187-95 PubMed
  2. ↑ Guzman, LM et al. (1992) FtsL, an essential cytoplasmic membrane protein involved in cell division in Escherichia coli. J. Bacteriol. 174 7716-28 PubMed
  3. ↑ 3.0 3.1 3.2 Li, GW et al. (2014) Quantifying absolute protein synthesis rates reveals principles underlying allocation of cellular resources. Cell 157 624-35 PubMed

Categories

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Retrieved from "https://ecoliwiki.org/colipedia/index.php?title=ftsL:Expression&oldid=1501180"