ada:Gene Product(s): Difference between revisions

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|{{Sub}}[[#Localization |Localization]]
|{{Sub}}[[#Localization |Localization]]
|{{Sub}}[[#Sequence|Sequence]]
|{{Sub}}[[#Sequence|Sequence]]
|{{Sub}}[[#Domains/Motifs/Modification Sites|Domains]]
|{{Sub}}[[#Structure|Structure]]
|{{Sub}}[[#Structure|Structure]]
|{{Sub}}[[#Gene Product Resources|Resources]]
|{{Sub}}[[#Gene Product Resources|Resources]]
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|-
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!{{table heading style}} |Standard name
!align=left {{table heading style}} |Standard name
|
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Ada product
Ada  
|-
|-
!{{table heading style}} |Synonyms
!align=left {{table heading style}} |Synonyms
|
||
fused DNA-binding transcriptional dual regulator product<ref name='LIB:Riley_2006'/>, O6-methylguanine-DNA methyltransferase product<ref name='LIB:Riley_2006'/>, B2213 product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, Ada product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>  
''fused DNA-binding transcriptional dual regulator<ref name='LIB:Riley_2006'/>, O6-methylguanine-DNA methyltransferase<ref name='LIB:Riley_2006'/>, B2213<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, Ada<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/> , ECK2205, JW2201, b2213''
|-
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!{{table heading style}} |Product description
!align=left {{table heading style}} |Product description
|
||
Ada transcriptional dual regulator<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>
Ada transcriptional dual regulator<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>


O6-methylguanine-DNA methyltransferase, transcription factor; inducible; DNA repair against methylating and alkylating agents; binds Zn(II)<ref name='LIB:EcoGene'/>
|-
|-
!{{table heading style}} |EC number (for enzymes)
!align=left {{table heading style}} |EC number (for enzymes)
|
||
** [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.63 2.1.1.63]<ref name='LIB:Riley_2006'/>
* [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.63 2.1.1.63]<ref name='LIB:Riley_2006'/>
|-
 
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{{Product_nomenclature_help}}
 
<protect><!--Nomenclature_notes--></protect>
<protect><!--Nomenclature_notes--></protect>
===Notes===
===Notes===


==Function==
==Function==
{{BacktoTop}}
{{BacktoTop}}
<protect>
<protect>
<big>'''Gene Ontology'''</big>
<big>'''Gene Ontology'''</big><br /><!--box uid=421aa90e079fa326b6494f812ad13e79.24595.Z46aa98cac84ca-->
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****************************************************************************************** -->{{Product_GO_help}}
{|class = 'sortable' {{Prettytable}} id='167716'
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="Z46aa98cac84ca"  class=" tableEdit GO_table_product dataTable" 
|- {{table_heading_style}}
|- {{table_heading_style}}
!Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
!|Qualifier!!GO ID!!GO term name!!Reference!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|-
|
 
|
GO:0003677
|
DNA binding
|
GOA:interpro<br />GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR004026
|
F
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0003677
|
DNA binding
|
GOA:spkw<br />SP_KW:GO_REF:0000004
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0238
|
F
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0003700
|
transcription factor activity
|
GOA:interpro<br />GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR000005
|
F
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0003824
|
catalytic activity
|
GOA:interpro<br />GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR014048
|
F
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0003908
|
methylated-DNA-(protein)-cysteine S-methyltransferase activity
|
GOA:interpro<br />GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR001497
|
F
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0003908
|
methylated-DNA-(protein)-cysteine S-methyltransferase activity
|
GOA:interpro<br />GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR008332
|
F
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0003908
|
methylated-DNA-(protein)-cysteine S-methyltransferase activity
|
GOA:spec<br />GO_REF:0000003
|
IEA: Inferred from Electronic Annotation
|
EC:2.1.1.63
|
F
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0005622
|
intracellular
|
GOA:interpro<br />GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR000005
|
C
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0006281
|
DNA repair
|
GOA:interpro<br />GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR001497
|
P
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0006281
|
DNA repair
|
GOA:interpro<br />GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR004026
|
P
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0006281
|
DNA repair
|
GOA:interpro<br />GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR008332
|
P
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0006281
|
DNA repair
|
GOA:interpro<br />GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR014048
|
P
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0006281
|
DNA repair
|
GOA:spkw<br />SP_KW:GO_REF:0000004
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0234
|
P
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0006350
|
transcription
|
GOA:spkw<br />SP_KW:GO_REF:0000004
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0804
|
P
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0006355
|
regulation of transcription, DNA-dependent
|
GOA:interpro<br />GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR000005
|
P
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0006355
|
regulation of transcription, DNA-dependent
|
GOA:interpro<br />GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR004026
|
P
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0006974
|
response to DNA damage stimulus
|
GOA:spkw<br />SP_KW:GO_REF:0000004
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0227
|
P
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0008270
|
zinc ion binding
|
PMID:1581309
|
IDA: Inferred from Direct Assay
|
 
|
F
|
N-terminal domain binds Zn
|
complete
|-
|
 
|
GO:0008168
|
methyltransferase activity
|
GOA:interpro<br />GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR004026
|
F
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0008168
|
methyltransferase activity
|
GOA:spkw<br />SP_KW:GO_REF:0000004
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0489
|
F
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0008270
|
zinc ion binding
|
GOA:interpro<br />GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR004026
|
F
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0008270
|
zinc ion binding
|
GOA:spkw<br />SP_KW:GO_REF:0000004
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0862
|
F
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0008270
|
zinc ion binding
|
PMID:1581309
|
IDA: Inferred from Direct Assay
|
 
|
F
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0016740
|
transferase activity
|
GOA:spkw<br />SP_KW:GO_REF:0000004
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0808
|
F
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-  
|
|
 
|
|
GO:0005737
GO:0043565
|
|
cytoplasm
sequence-specific DNA binding
|
|
GOA:interpro<br />GO_REF:0000002
|
|
IEA: Inferred from Electronic Annotation
|
|
InterPro:IPR000005
|
|
C
F
|
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.  
Seeded from [[EcoCyc]] (v14.0)
|
|
required fields missing 
complete
|-
|-  
|
|
 
|
|
GO:0006281
GO:0003908
|
|
DNA repair
methylated-DNA-(protein)-cysteine S-methyltransferase activity
|
|
PMID:6754717
|
|
IDA: Inferred from Direct Assay
|
|
 
|
|
P
F
|
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
 
|
|
required fields missing 
complete
|-
|-  
|
|
 
|
|
GO:0006350
GO:0005625
|
|
transcription
soluble fraction
|
|
PMID:6754717
|
|
IDA: Inferred from Direct Assay
|
|
 
|
|
P
C
|
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
 
|
|
required fields missing 
complete
|-
|-  
|
|
 
|
|
GO:0016563
GO:0006307
|
|
transcription activator activity
DNA dealkylation involved in DNA repair
|
|
PMID:6754717
|
|
IDA: Inferred from Direct Assay
|
|
 
|
|
F
P
|
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
 
|
|
required fields missing 
complete
|-
|-  
|
|
 
|
|
GO:0016564
GO:0006307
|
|
transcription repressor activity
DNA dealkylation involved in DNA repair
|
|
PMID:
282622
|
|
IDA: Inferred from Direct Assay
|
|
 
|
|
F
P
|
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
 
|
|
required fields missing 
<div class="mw-parser-output"><p>complete
|-class='sortbottom'
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<span id="Interactions"></span><big>'''Interactions'''</big>
<span id="Interactions"></span><big>'''Interactions'''</big>
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!Partner Type!!Partner!!Notes
!|Partner Type!!Partner!!Notes!!References!!Evidence
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Protein
|
rplB<br />
|
 
|
PMID:16606699
|
Experiment(s):EBI-1142195<br />
|-
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Protein
|
rpsC<br />
|
 
|
PMID:16606699
|
Experiment(s):EBI-1142195<br />
|-
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Protein
|
moeB<br />
|
 
|
PMID:16606699
|
Experiment(s):EBI-1142195<br />
|-
|
Protein
|
fur<br />
|
 
|
PMID:16606699
|
Experiment(s):EBI-1142195<br />
|-
|
Protein
|
rpsG<br />
|
 
|
PMID:16606699
|
Experiment(s):EBI-1142195<br />
|-
|
Protein
|
ybiA<br />
|
 
|
PMID:16606699
|
Experiment(s):EBI-1142195<br />
 
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<!--Function_notes--></protect>
<!--Function_notes--></protect>
===Notes===
===Notes===
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!Compartment!!Description!!Evidence!!Notes
!|Compartment!!Description!!Evidence!!Reference/Source!!Notes
|-
|-  
|
|
membrane
membrane
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From EcoCyc<ref name='LIB:EcoCyc11.1'/>   
From EcoCyc<ref name='LIB:EcoCyc11.1'/>  
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{|{{Prettytable}}
{|   id="N46aa98eec4384"  class=" tableEdit Product_phys_prop_table" 
 
|-
!align=left {{table heading style}} |Name
||
 
|-
|-
!{{table heading style}} |Sequence
!align=left {{table heading style}} |Sequence
|
||
[http://biocyc.org/ECOLI/sequence-aa?object=PD00230 at EcoCyc]  
[http://biocyc.org/ECOLI/sequence-aa?object=PD00230 at EcoCyc]  
<pre>MKKATCLTDD QRWQSVLARD PNADGEFVFA VRTTGIFCRP SCRARHALRE NVSFYANASE
<pre>MKKATCLTDD QRWQSVLARD PNADGEFVFA VRTTGIFCRP SCRARHALRE NVSFYANASE
Line 249: Line 784:
KAVRAVASAC AANKLAIIIP CHRVVRGDGT LSGYRWGVSR KAQLLRREAE NEER</pre>  
KAVRAVASAC AANKLAIIIP CHRVVRGDGT LSGYRWGVSR KAQLLRREAE NEER</pre>  
|-
|-
!{{table heading style}} |Mol. Wt
!align=left {{table heading style}} |Length
|
||
39.32 kDa (calc) <ref name='LIB:EcoCyc10.6'/>
354
|-
|-
!{{table heading style}} |pI
!align=left {{table heading style}} |Mol. Wt
|
||
8.69<ref name='LIB:EcoCyc10.6'/>
39.323 kDa
|-
|-
!{{table heading style}} |Extinction coefficient
!align=left {{table heading style}} |pI
|
||
36440 - 37940 (calc based on 6 Y, 5 W, and 12 C residues)  
8.8 (calculated)
|-
|-
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!align=left {{table heading style}} |Extinction coefficient
||
36,440 - 37,940 (calc based on 6 Y, 5 W, and 12 C residues)
 
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<big>'''Domains/Motifs/Modification Sites'''</big>
 
<!--box uid=421aa90e079fa326b6494f812ad13e79.24595.Z46aa98ef3d7e3-->
<span id="Domains/Motifs/Modification Sites"><big>'''Domains/Motifs/Modification Sites'''</big></span><table>
<tr align="left" valign="top">
<td><!--box uid=421aa90e079fa326b6494f812ad13e79.24595.Z46aa98ef3d7e3-->
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|- {{table heading style}}
!Type!!Residues!!Description!!Notes
!|Type!!Residues!!Description!!Notes!!References
|-class='sortbottom'
|-
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|
Domain
|
10..75
|
[http://pfam.janelia.org/family/PF02805 PF02805  Metal binding domain of Ada]
|
 
|
PMID:19920124
|-
|
Domain
|
95..133
|
[http://pfam.janelia.org/family/PF00165 PF00165  Bacterial regulatory helix-turn-helix proteins, AraC family]
|
 
|
PMID:19920124
|-
|
Domain
|
140..182
|
[http://pfam.janelia.org/family/PF00165 PF00165  Bacterial regulatory helix-turn-helix proteins, AraC family]
|
 
|
PMID:19920124
|-
|
Domain
|
269..353
|
[http://pfam.janelia.org/family/PF01035 PF01035  6-O-methylguanine DNA methyltransferase, DNA binding domain]
|
 
|
PMID:19920124
|-
|
Domain
|
188..266
|
[http://pfam.janelia.org/family/PF02870 PF02870  6-O-methylguanine DNA methyltransferase, ribonuclease-like domain]
|
 
|
PMID:19920124
 
|- class="tableEdit_footer"
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{{Product_domains_motifs_help}}
<td>
<!-- motifs column 2-->
<motif_map/>
</td>
</tr>
</table>


<span id="Structure"></span><big>'''Structure'''</big>
<table><tr valign='top'><td>
<span id='Structure'></span><big>'''Structure'''</big><br />
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{|class = 'sortable' {{Prettytable}} id='113678'
{|   id="M46a82cf901614"  class=" tableEdit Product_structure_table" 
|- {{table heading style}}
 
!Structure Type!!Organism!!Accession!!Residues covered!!Notes
|-
|-class='sortbottom'
!align=left {{table heading style}} |Structures
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.24595.M46a82cf901614&page=24595&pagename={{FULLPAGENAMEE}} edit table] || || || ||
||
<beststructure>
gene=ada
taxon=562,83333
</beststructure>
|-
!align=left {{table heading style}} |Models
||
View models at:
* [http://www.proteinmodelportal.org/query/uniprot/P06134 Protein Model Portal]
* [http://bioinf.cs.ucl.ac.uk/genome3d/read?uniprot_id=P06134 PSI-PRED (P06134)]
* [http://spock.genes.nig.ac.jp/~genome/cgi-bin/gkeytext.pl.cgi?A&QUERY1=P06134 Genomes TO Protein (P06134)] (GTOP)
 
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<!--Structure_notes--></protect>
</td><td></protect>
==== Structure figures ====
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
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===Notes===
===Notes===


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===Notes===
===Notes===
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|- {{table_heading_style}}
|- {{table_heading_style}}
!Database!!Accession!!Notes
!|Database!!Accession!!Notes
|-
|-
|
[http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/protein/16130150 GI:16130150]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/protein/NP_416717.1 GeneID:946710]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[[ASAP]]
|
ASAP:ABE-0007311
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[http://www.pir.uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
|
[http://www.uniprot.org/uniprot/P06134 UniProtKB/Swiss-Prot:P06134]
|
Escherichia coli str. K-12 substr. MG1655
|-  
|
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
|
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=PD00230 EcoCyc:PD00230] 
EcoCyc:EG10029
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|-  
|
|
[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
[[EcoGene]]
|
|
[[uniprot:P06134|UniProt:P06134]] 
EcoGene:EG10029
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|-  
|
|
[http://pfam.sanger.ac.uk/ PFAM] ([[PFAM|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI (Gene)] ([[NCBI (Gene)|EcoliWiki Page]])
|
|
[http://pfam.sanger.ac.uk/family?acc=PF02805 PFAM:PF02805]
[http://www.ncbi.nlm.nih.gov/gene?term=946710 GeneID:946710]
|
|
Escherichia coli str. K-12 substr. MG1655
|-
|-  
|
|
[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])
[[RegulonDB]]
|
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_416717 RefSeq:NP_416717] 
RegulonDB:ECK120000026
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|-  
|
|
[http://modbase.compbio.ucsf.edu ModBase] ([[ModBase|EcoliWiki Page]])
[[EchoBASE]]
|
|
[http://salilab.org/modbase/searchbyid?databaseID=P06134 ModBase:P06134] 
EchoBASE:EB0028
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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===Notes===
===Notes===
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{{RefHelp}}
{{RefHelp}}
<references/>
<references />
__NOTOC__
__NOTOC__
</noinclude>
===Categories===
===Categories===
{{BacktoTop}}
{{BacktoTop}}
[[Category:Proteins]]
[[Category:Proteins]]
[[Category:GO:0005737_!_cytoplasm]][[Category:GO:0006281_!_DNA repair]][[Category:GO:0006350_!_transcription]][[Category:GO:0016563_!_transcription activator activity]][[Category:GO:0016564_!_transcription repressor activity]][[Category:Location:membrane]]

Latest revision as of 19:15, 6 June 2024

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

Ada

Synonyms

fused DNA-binding transcriptional dual regulator[1], O6-methylguanine-DNA methyltransferase[1], B2213[2][1], Ada[2][1] , ECK2205, JW2201, b2213

Product description

Ada transcriptional dual regulator[2][3]

O6-methylguanine-DNA methyltransferase, transcription factor; inducible; DNA repair against methylating and alkylating agents; binds Zn(II)[4]

EC number (for enzymes)
edit table


Notes

Function

[back to top]


Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004026

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0238

F

Seeded from EcoCyc (v14.0)

complete

GO:0003700

transcription factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000005

F

Seeded from EcoCyc (v14.0)

complete

GO:0003824

catalytic activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR014048

F

Seeded from EcoCyc (v14.0)

complete

GO:0003908

methylated-DNA-(protein)-cysteine S-methyltransferase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001497

F

Seeded from EcoCyc (v14.0)

complete

GO:0003908

methylated-DNA-(protein)-cysteine S-methyltransferase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008332

F

Seeded from EcoCyc (v14.0)

complete

GO:0003908

methylated-DNA-(protein)-cysteine S-methyltransferase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:2.1.1.63

F

Seeded from EcoCyc (v14.0)

complete

GO:0005622

intracellular

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000005

C

Seeded from EcoCyc (v14.0)

complete

GO:0006281

DNA repair

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001497

P

Seeded from EcoCyc (v14.0)

complete

GO:0006281

DNA repair

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004026

P

Seeded from EcoCyc (v14.0)

complete

GO:0006281

DNA repair

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008332

P

Seeded from EcoCyc (v14.0)

complete

GO:0006281

DNA repair

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR014048

P

Seeded from EcoCyc (v14.0)

complete

GO:0006281

DNA repair

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0234

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0804

P

Seeded from EcoCyc (v14.0)

complete

GO:0006355

regulation of transcription, DNA-dependent

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000005

P

Seeded from EcoCyc (v14.0)

complete

GO:0006355

regulation of transcription, DNA-dependent

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004026

P

Seeded from EcoCyc (v14.0)

complete

GO:0006974

response to DNA damage stimulus

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0227

P

Seeded from EcoCyc (v14.0)

complete

GO:0008270

zinc ion binding

PMID:1581309[5]

IDA: Inferred from Direct Assay

F

N-terminal domain binds Zn

complete

GO:0008168

methyltransferase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004026

F

Seeded from EcoCyc (v14.0)

complete

GO:0008168

methyltransferase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0489

F

Seeded from EcoCyc (v14.0)

complete

GO:0008270

zinc ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004026

F

Seeded from EcoCyc (v14.0)

complete

GO:0008270

zinc ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0862

F

Seeded from EcoCyc (v14.0)

complete

GO:0008270

zinc ion binding

PMID:1581309[5]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0016740

transferase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0808

F

Seeded from EcoCyc (v14.0)

complete

GO:0043565

sequence-specific DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000005

F

Seeded from EcoCyc (v14.0)

complete

GO:0003908

methylated-DNA-(protein)-cysteine S-methyltransferase activity

PMID:6754717[6]

IDA: Inferred from Direct Assay

F

complete

GO:0005625

soluble fraction

PMID:6754717[6]

IDA: Inferred from Direct Assay

C

complete

GO:0006307

DNA dealkylation involved in DNA repair

PMID:6754717[6]

IDA: Inferred from Direct Assay

P

complete

GO:0006307

DNA dealkylation involved in DNA repair

PMID:282622[7]

IDA: Inferred from Direct Assay

P

complete

edit table

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

rplB

PMID:16606699[8]

Experiment(s):EBI-1142195

Protein

rpsC

PMID:16606699[8]

Experiment(s):EBI-1142195

Protein

moeB

PMID:16606699[8]

Experiment(s):EBI-1142195

Protein

fur

PMID:16606699[8]

Experiment(s):EBI-1142195

Protein

rpsG

PMID:16606699[8]

Experiment(s):EBI-1142195

Protein

ybiA

PMID:16606699[8]

Experiment(s):EBI-1142195
edit table


Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

membrane

From EcoCyc[3]


edit table


Notes

Structure and Physical Properties

[back to top]


Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MKKATCLTDD QRWQSVLARD PNADGEFVFA VRTTGIFCRP SCRARHALRE NVSFYANASE
ALAAGFRPCK RCQPEKANAQ QHRLDKITHA CRLLEQETPV TLEALADQVA MSPFHLHRLF
KATTGMTPKA WQQAWRARRL RESLAKGESV TTSILNAGFP DSSSYYRKAD ETLGMTAKQF
RHGGENLAVR YALADCELGR CLVAESERGI CAILLGDDDA TLISELQQMF PAADNAPADL
MFQQHVREVI ASLNQRDTPL TLPLDIRGTA FQQQVWQALR TIPCGETVSY QQLANAIGKP
KAVRAVASAC AANKLAIIIP CHRVVRGDGT LSGYRWGVSR KAQLLRREAE NEER
Length

354

Mol. Wt

39.323 kDa

pI

8.8 (calculated)

Extinction coefficient

36,440 - 37,940 (calc based on 6 Y, 5 W, and 12 C residues)

edit table


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

10..75

PF02805 Metal binding domain of Ada

PMID:19920124[9]

Domain

95..133

PF00165 Bacterial regulatory helix-turn-helix proteins, AraC family

PMID:19920124[9]

Domain

140..182

PF00165 Bacterial regulatory helix-turn-helix proteins, AraC family

PMID:19920124[9]

Domain

269..353

PF01035 6-O-methylguanine DNA methyltransferase, DNA binding domain

PMID:19920124[9]

Domain

188..266

PF02870 6-O-methylguanine DNA methyltransferase, ribonuclease-like domain

PMID:19920124[9]

edit table

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=ada taxon=562,83333 </beststructure>

Models

View models at:

edit table

Structure figures

Notes

Gene Product Resources

[back to top]


See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
edit table


Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130150

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946710

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007311

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P06134

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10029

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10029

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946710

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000026

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0028

Escherichia coli str. K-12 substr. MG1655

edit table


Notes

Links

[back to top]




References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 Myers, LC et al. (1992) Zinc binding by the methylation signaling domain of the Escherichia coli Ada protein. Biochemistry 31 4541-7 PubMed
  6. ↑ 6.0 6.1 6.2 Demple, B et al. (1982) Repair of alkylated DNA in Escherichia coli. Physical properties of O6-methylguanine-DNA methyltransferase. J. Biol. Chem. 257 13776-80 PubMed
  7. ↑ Schendel, PF & Robins, PE (1978) Repair of O6-methylguanine in adapted Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 75 6017-20 PubMed
  8. ↑ 8.0 8.1 8.2 8.3 8.4 8.5 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  9. ↑ 9.0 9.1 9.2 9.3 9.4 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

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