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{|   id="Z49b023ab6b9bb"  class=" tableEdit New_Quickview_Table" 
 
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!{{table_heading_style}} |Gene Name
!align=left {{table_heading_style}} |Standard Name
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[[rpsL:Gene|''rpsL]]'' (synonyms: ''ECK3329, b3342, JW3304, asuB, strA'')<ref name='LIB:Riley_2006'/>
''rpsL''
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!{{table_heading_style}} |Product(s)  
!align=left {{table_heading_style}} |Gene Synonym(s)
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[[rpsL:Gene_Product(s)|RpsL product]]<ref name='LIB:Riley_2006'/>: 30S ribosomal subunit protein S12 product, B3342 product, StrA product, AsuB product
''ECK3329, b3342, JW3304, asuB, strA''<ref name='LIB:Riley_2006'/>, strA''
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!{{table_heading_style}} |Description
!align=left {{table_heading_style}} |Product Desc.
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30S ribosomal subunit protein S12<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>  
30S ribosomal subunit protein S12<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>;


Component of [[:Category:Complex:30S ribosomal subunit|30S ribosomal subunit]]<ref name='LIB:EcoCyc10.6'/>,[[:Category:Complex:ribosome|ribosome]]<ref name='LIB:EcoCyc10.6'/>
Component of [[:Category:Complex:30S ribosomal subunit|30S ribosomal subunit]]<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>; [[:Category:Complex:ribosome|ribosome]]<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>


30S ribosomal subunit protein S12; RNA chaperone<ref name='LIB:EcoGene'/>
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!align=left {{table_heading_style}} |Product Synonyms(s)
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''30S ribosomal subunit protein S12<ref name='LIB:Riley_2006'/>, B3342<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, StrA<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, AsuB<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, RpsL<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/> , asuB, ECK3329, JW3304, strA, b3342''
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!align=left {{table_heading_style}} |Function from GO
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<GO_nr />
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!{{table_heading_style}} |Knock-Out Phenotype
!align=left {{table_heading_style}} |Knock-Out Phenotype
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|-
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!{{table_heading_style}} |Regulation/Expression
!align=left {{table_heading_style}} |Regulation/Expression
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transcription unit(s): [[:Category:Txn_unit:rpsLG-fusA-tufA|rpsLG-fusA-tufA]]<ref name='LIB:EcoCyc10.6'/>  
transcription unit(s): [[:Category:Txn_unit:rpsLG-fusA-tufA|rpsLG-fusA-tufA]]<ref name='LIB:EcoCyc10.6'/>  
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!{{table_heading_style}} |Regulation/Activity
!align=left {{table_heading_style}} |Regulation/Activity
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!align=left {{table_heading_style}} |Quick Links
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[[File:porteco.png|link=http://porteco.org/AjaxSearch.jsp?searchString=rpsL&s=search]] [[File:EcoCyc.gif|link=http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10911]] [[File:regulondb.jpg|link=http://regulondb.ccg.unam.mx/gene?term=ECK120000902&organism=ECK12&format=jsp&type=gene ]] <br/><br/> [[File:dnadisplay.png|link=http://ecoliwiki.net/tools/display_dna.php?gene=rpsL&loc=MG1655%3A3472574..3472200,W3110%3A4165864..4166238,REL606%3A3402457..3402083,BW2952%3A3359357..3359731]] [[File:proteindisplay.png|link=http://ecoliwiki.net/tools/display_protein.php?f=NC_000913&start=3359357&end=3359731&gene=rpsL&aa=matvnqlvrkprarkvaksnvpaleacpqkrgvctrvytttpkkpnsalrkvcrvrltngfevtsyiggeghnlqehsvilirggrvkdlpgvryhtvrgaldcsgvkdrkqarskygvkrpka]]&nbsp;&nbsp;  [[File:pubmed.jpg|link=http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=Search&Term=rpsL+AND+escherichia+coli]]&nbsp;&nbsp;  [[File:textpresso.jpg|link=http://textpresso.tamu.edu/cgi-bin/textpresso/search?q=rpsL]]&nbsp;&nbsp;
 
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''RpsL'' is a ribosomal gene that encodes the highly conserved ''rps12'' protein of the ribosomal accuracy center in the ''Escherichia coli''. Numerous mutations in the ''rpsL'' gene generate resistance to, and in some instances dependence on, high levels of streptomycin. <ref name='PMID:10209746'/>
''RpsL'' is a ribosomal gene that encodes the highly conserved ''rps12'' protein of the ribosomal accuracy center in the ''Escherichia coli''. Numerous mutations in the ''rpsL'' gene generate resistance to, and in some instances dependence on, high levels of streptomycin. <ref name='PMID:10209746'/>
==References==
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Mutations in regions 41-45 (except when lys42 is replaced with arg), 87-93 and 53 result in streptomycin resistance.  A point mutation at 262 from A to G sustains protein synthesis in late stationary phase and increases the stability of the 70S complex in low magnesium concentrations.<ref name='LIB:EcoGene'/>

Latest revision as of 21:46, 27 May 2013

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
References Suggestions
Standard Name

rpsL

Gene Synonym(s)

ECK3329, b3342, JW3304, asuB, strA[1], strA

Product Desc.

30S ribosomal subunit protein S12[2][3];

Component of 30S ribosomal subunit[2][3]; ribosome[2][3]

30S ribosomal subunit protein S12; RNA chaperone[4]

Product Synonyms(s)

30S ribosomal subunit protein S12[1], B3342[2][1], StrA[2][1], AsuB[2][1], RpsL[2][1] , asuB, ECK3329, JW3304, strA, b3342

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): rpsLG-fusA-tufA[2]

Regulation/Activity
Quick Links



        

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RpsL is a ribosomal gene that encodes the highly conserved rps12 protein of the ribosomal accuracy center in the Escherichia coli. Numerous mutations in the rpsL gene generate resistance to, and in some instances dependence on, high levels of streptomycin. [5]

References

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  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ Toivonen, JM et al. (1999) Modelling in Escherichia coli of mutations in mitoribosomal protein S12: novel mutant phenotypes of rpsL. Mol. Microbiol. 31 1735-46 PubMed


Mutations in regions 41-45 (except when lys42 is replaced with arg), 87-93 and 53 result in streptomycin resistance. A point mutation at 262 from A to G sustains protein synthesis in late stationary phase and increases the stability of the 70S complex in low magnesium concentrations.[1]

  1. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
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