BW25113: Difference between revisions
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#REDIRECT [[Category:Strain:BW25113]] | |||
{{Strain Help}} | |||
== | ==Alternate Names == | ||
== | ==Chromosomal Genotype == | ||
∆''(araD-araB)567'', ∆''lacZ4787''(::rrnB-3), lambda<sup>-</sup>, ''rph-1'', ∆''(rhaD-rhaB)568'', ''hsdR514'' | |||
==Plasmids == | |||
== | ==Phage == | ||
==Construction | ==Phenotypes == | ||
==Construction == | |||
BW25113 is a derivative of the F<sup>-</sup>, λ<sup>-</sup>, ''E. coli'' K-12 strain [http://cgsc2.biology.yale.edu/Strain.php?ID=7024 BD792 (CGSC6159)]<ref name='PMID:10829079'/>, which is a derivative of ''E. coli'' K-12 W1485. According to the CGSC, BD792 carries ''rpoS396''(Am), but this mutation is not listed in the published genotype for BW25113. | |||
==Notes== | ==Notes== | ||
BW25113 was used to make the Keio Collection of single-gene knockouts. <ref name=PMID:16738554/> | |||
<ref name=PMID:10829079/> Available from the Coli Genetics Stock Center: [http://cgsc2.biology.yale.edu/Strain.php?ID=64667 CGSC#7636] | <ref name=PMID:10829079/> Available from the Coli Genetics Stock Center: [http://cgsc2.biology.yale.edu/Strain.php?ID=64667 CGSC#7636] | ||
The genotype for BW25113 in Datsenko et al. <ref name=PMID:10829079/> said the strain is lacI<sup>Q</sup>, but it was later shown to be lacI<sup>+</sup> Baba et al. (Supplementary Table 1)<ref name=PMID:16738554/>. | |||
According to the CGSC, ''lacZ4787(del)'' has 4 tandem copies of the rrnB transcriptional terminator inserted by gene replacement into the region extending from near the SacII site near the N-terminus of ''lacZ'' through the promoter. | |||
==References== | ==References== | ||
{{RefHelp}} | {{RefHelp}} | ||
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[[Category:Strains]] | [[Category:Strains]] [[Category:Strain:W1485]] | ||
Latest revision as of 18:43, 1 April 2013
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Alternate Names
Chromosomal Genotype
∆(araD-araB)567, ∆lacZ4787(::rrnB-3), lambda-, rph-1, ∆(rhaD-rhaB)568, hsdR514
Plasmids
Phage
Phenotypes
Construction
BW25113 is a derivative of the F-, λ-, E. coli K-12 strain BD792 (CGSC6159)[1], which is a derivative of E. coli K-12 W1485. According to the CGSC, BD792 carries rpoS396(Am), but this mutation is not listed in the published genotype for BW25113.
Notes
BW25113 was used to make the Keio Collection of single-gene knockouts. [2] [1] Available from the Coli Genetics Stock Center: CGSC#7636 The genotype for BW25113 in Datsenko et al. [1] said the strain is lacIQ, but it was later shown to be lacI+ Baba et al. (Supplementary Table 1)[2].
According to the CGSC, lacZ4787(del) has 4 tandem copies of the rrnB transcriptional terminator inserted by gene replacement into the region extending from near the SacII site near the N-terminus of lacZ through the promoter.
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 Datsenko, KA & Wanner, BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97 6640-5 PubMed
- ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed