DNA Mismatch Repair: Difference between revisions
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*Physical Damage to Bases | *Physical Damage to Bases | ||
*Homologous Recombination | *Homologous Recombination | ||
Review Articles<ref name='PMID:8811176'/> | |||
==Components== | ==Components== | ||
*[[mutS:Gene_Product(s)|MutS]]- Mismatch Recognition and Specificity | *d(GATC) sequences<ref name='PMID:3550791'/> | ||
*[[mutH:Gene_Product(s)|MutH]] | *[[mutS:Gene_Product(s)|MutS]]<ref name='PMID:1542666'/><ref name='PMID:3014530'/>- Mismatch Recognition and Specificity | ||
*[[mutL:Gene_Product(s)|MutL]] | *[[mutH:Gene_Product(s)|MutH]]<ref name='PMID:9880500'/>- d(GATC)-specific Endonuclease | ||
*[[mutL:Gene_Product(s)|MutL]]<ref name='PMID:10734142'/> | |||
==Function== | ==Function== | ||
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==Cell Biology== | ==Cell Biology== | ||
*Recognition of a Mismatch<ref name='PMID:14634210'/><ref name='PMID:8633074'/> | |||
*MutS binds DNA non-specifically and bends it in search of a mismatch | **MutS binds DNA non-specifically and bends it in search of a mismatch | ||
*MutS undergoes a conformation change when bound to a mismatched duplex to form an initial recognition complex (DNA is kinked) | **MutS undergoes a conformation change when bound to a mismatched duplex to form an initial recognition complex (DNA is kinked) | ||
*MutS undergoes a further conformation change to a final recognition complex (DNA is unbent) | **MutS undergoes a further conformation change to a final recognition complex (DNA is unbent) | ||
*Excision of the Mismatch | |||
**MutH endonuclease is activated by MutS, MutL, ATP, Mg<sup>2+</sup>, and a mismatched piece of DNA<ref name='PMID:1601880'/> | |||
**Mismatch repair excision is bidirectional<ref name='PMID:8389365'/><ref name='PMID:8505311'/> | |||
==Experimental Resources== | ==Experimental Resources== | ||
Latest revision as of 20:33, 31 August 2010
General Description
"A system for the correction of errors in which an incorrect base, which cannot form hydrogen bonds with the corresponding base in the parent strand, is incorporated into the daughter strand. The mismatch repair system promotes genomic fidelity by repairing base-base mismatches, insertion-deletion loops and heterologies generated during DNA replication and recombination." [ISBN:0198506732 "Oxford Dictionary of Biochemistry and Molecular Biology"]
Corrects mismatched and unpaired bases arising from:
- Replication Errors
- Physical Damage to Bases
- Homologous Recombination
Review Articles[1]
Components
- d(GATC) sequences[2]
- MutS[3][4]- Mismatch Recognition and Specificity
- MutH[5]- d(GATC)-specific Endonuclease
- MutL[6]
Function
Regulation
Cell Biology
- Recognition of a Mismatch[7][8]
- MutS binds DNA non-specifically and bends it in search of a mismatch
- MutS undergoes a conformation change when bound to a mismatched duplex to form an initial recognition complex (DNA is kinked)
- MutS undergoes a further conformation change to a final recognition complex (DNA is unbent)
- Excision of the Mismatch
Experimental Resources
Comparison with other Organisms
Notes
Related GO Terms
References
- ↑ Modrich, P & Lahue, R (1996) Mismatch repair in replication fidelity, genetic recombination, and cancer biology. Annu. Rev. Biochem. 65 101-33 PubMed
- ↑ Lahue, RS et al. (1987) Requirement for d(GATC) sequences in Escherichia coli mutHLS mismatch correction. Proc. Natl. Acad. Sci. U.S.A. 84 1482-6 PubMed
- ↑ Parker, BO & Marinus, MG (1992) Repair of DNA heteroduplexes containing small heterologous sequences in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 89 1730-4 PubMed
- ↑ Su, SS & Modrich, P (1986) Escherichia coli mutS-encoded protein binds to mismatched DNA base pairs. Proc. Natl. Acad. Sci. U.S.A. 83 5057-61 PubMed
- ↑ Hall, MC & Matson, SW (1999) The Escherichia coli MutL protein physically interacts with MutH and stimulates the MutH-associated endonuclease activity. J. Biol. Chem. 274 1306-12 PubMed
- ↑ Spampinato, C & Modrich, P (2000) The MutL ATPase is required for mismatch repair. J. Biol. Chem. 275 9863-9 PubMed
- ↑ Wang, H et al. (2003) DNA bending and unbending by MutS govern mismatch recognition and specificity. Proc. Natl. Acad. Sci. U.S.A. 100 14822-7 PubMed
- ↑ Smith, J & Modrich, P (1996) Mutation detection with MutH, MutL, and MutS mismatch repair proteins. Proc. Natl. Acad. Sci. U.S.A. 93 4374-9 PubMed
- ↑ Au, KG et al. (1992) Initiation of methyl-directed mismatch repair. J. Biol. Chem. 267 12142-8 PubMed
- ↑ Cooper, DL et al. (1993) Methyl-directed mismatch repair is bidirectional. J. Biol. Chem. 268 11823-9 PubMed
- ↑ Grilley, M et al. (1993) Bidirectional excision in methyl-directed mismatch repair. J. Biol. Chem. 268 11830-7 PubMed