proP:Gene Product(s): Difference between revisions

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!align=left {{table heading style}} |Synonyms
!align=left {{table heading style}} |Synonyms
||
||
proline/glycine betaine transporter<ref name='LIB:Riley_2006'/>, B4111<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, ProP<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>  
''proline/glycine betaine transporter<ref name='LIB:Riley_2006'/>, B4111<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, ProP<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/> , ECK4104, JW4072, b4111''
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!align=left {{table heading style}} |Product description
!align=left {{table heading style}} |Product description
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{|   id="G46ab1d4ce7da9"  class=" tableEdit GO_table_product dataTable"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="G46ab1d4ce7da9"  class=" tableEdit GO_table_product dataTable"   
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
!|Qualifier!!GO ID!!GO term name!!Reference!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-  
|-  
|
|


|
|
GO:0006810
GO:0016021
|
|
transport
integral to membrane
|
|
PMID:6355059
PMID:10026245
|
|
IGI: Inferred from Genetic Interaction
IDA: Inferred from Direct Assay
|
|


|
|
P
C
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
 
|
|
complete  
complete  
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|
|
GO:0015292
GO:0005886
|
|
uniporter activity
plasma membrane
|
|
PMID:15205426
PMID:10026245
|
|
IGI: Inferred from Genetic Interaction
IDA: Inferred from Direct Assay
|
|


|
|
F
C
|
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
 
|
|
complete  
complete  
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|
|
GO:0015295
GO:0005886
|
plasma membrane
|
GOA:spkw<br />SP_KW:GO_REF:0000004
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0997
|
|
solute:hydrogen symporter activity
C
|
|
PMID:3049595
Seeded from [[EcoCyc]] (v14.0)
|
|
IGI: Inferred from Genetic Interaction
complete
|-
|
|


|
|
F
GO:0005886
|
plasma membrane
|
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
GOA:spkw<br />SP_KW:GO_REF:0000004
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-1003
|
C
|
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
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|
|
GO:0042538
GO:0016021
|
|
hyperosmotic salinity response
integral to membrane
|
|
PMID:3082857
GOA:interpro<br />GO_REF:0000002
|
|
IGI: Inferred from Genetic Interaction
IEA: Inferred from Electronic Annotation
|
|
 
InterPro:IPR004736
|
|
P
C
|
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
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integral to membrane
integral to membrane
|
|
PMID:10026245
GOA:interpro<br />GO_REF:0000002
|
|
IDA: Inferred from Direct Assay
IEA: Inferred from Electronic Annotation
|
|
 
InterPro:IPR005828
|
|
C
C
|
|
 
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
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|
|
GO:0016020
GO:0016021
|
|
membrane
integral to membrane
|
|
GO_REF:0000002<br />GO_REF:0000004<br />GO_REF:0000004
GOA:spkw<br />SP_KW:GO_REF:0000004
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
InterPro:IPR005829<br />SP_KW:KW-0397<br />SP_KW:KW-0472
SP_KW:KW-0812
|
|
C
C
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
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|
|
GO:0006810
GO:0016021
|
|
transport
integral to membrane
|
|
GO_REF:0000002<br />GO_REF:0000002<br />GO_REF:0000002<br />GO_REF:0000002<br />GO_REF:0000004
GO_REF:0000023
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
InterPro:IPR004736<br />InterPro:IPR005828<br />InterPro:IPR005829<br />InterPro:IPR007114<br />SP_KW:KW-0813
SP_SL:SL-9909
|
|
P
C
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
|-  
|-  
|
|
 
|
|
GO:0006561
GO:0055085
|
|
proline biosynthetic process
transmembrane transport
|
|
GOA:interpro<br />GO_REF:0000002
|
|
IEA: Inferred from Electronic Annotation
|
|
InterPro:IPR005828
|
|
P  
P
|
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.  
Seeded from [[EcoCyc]] (v14.0)
|
|
required fields missing 
complete
|-  
|-  
|
|


|
|
GO:0005886
GO:0055085
|
|
plasma membrane
transmembrane transport
|
|
GO_REF:0000004<br />GO_REF:0000004<br />GO_REF:0000023
GOA:interpro<br />GO_REF:0000002
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
SP_KW:KW-0997<br />SP_KW:KW-1003<br />SP_SL:SL-0037
InterPro:IPR005829
|
|
C
P
|
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
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|
|
GO:0015293
GO:0006865
|
|
symporter activity
amino acid transport
|
|
GO_REF:0000004
PMID:10026245
|
|
IEA: Inferred from Electronic Annotation
IDA: Inferred from Direct Assay
|
|
SP_KW:KW-0769
 
|
|
F
P
|
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
 
|
|
complete  
complete  
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|
|
GO:0016021
GO:0006865
|
|
integral to membrane
amino acid transport
|
|
GO_REF:0000002<br />GO_REF:0000002<br />GO_REF:0000002<br />GO_REF:0000004
PMID:10026245
|
|
IEA: Inferred from Electronic Annotation
IDA: Inferred from Direct Assay
|
|
InterPro:IPR004736<br />InterPro:IPR005828<br />InterPro:IPR007114<br />SP_KW:KW-0812
 
|
|
C
P
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
 
|
|
complete  
complete  
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{|   id="B46ab1d597e0a3"  class=" tableEdit Product_interactions_table dataTable"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="B46ab1d597e0a3"  class=" tableEdit Product_interactions_table dataTable"   
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!|Partner Type!!Partner!!Notes!!References!!Evidence
!|Partner Type!!Partner!!Notes!!References!!Evidence
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="G46ab1d5a53983"  class=" tableEdit Product_localization_table"   
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!|Compartment!!Description!!Evidence!!Source!!Notes
!|Compartment!!Description!!Evidence!!Reference/Source!!Notes
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!align=left {{table heading style}} |Name
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!|Type!!Residues!!Description!!Notes!!References
!|Type!!Residues!!Description!!Notes!!References
|-
|
Domain
|
25..453
|
[http://pfam.janelia.org/family/PF00083 PF00083  Sugar (and other) transporter]
|
|
PMID:19920124
|-
|
Domain
|
454..499
|
[http://pfam.janelia.org/family/PF08946 PF08946  Osmosensory transporter coiled coil]
|
|
PMID:19920124


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View models at:
View models at:
* [http://dragon.bio.purdue.edu/ecolpredict2/localsearch/cgi-bin/search3.cgi?q=recA EcoliPredict]
* [http://www.proteinmodelportal.org/query/uniprot/P0C0L7 Protein Model Portal]
* [http://modbase.compbio.ucsf.edu/modbase-cgi/model_search.cgi?searchmode=default&displaymode=moddetail&searchproperties=ALL&searchvalue=recA&organism=562&organismtext= ModBase]
* [http://bioinf.cs.ucl.ac.uk/genome3d/read?uniprot_id=P0C0L7 PSI-PRED (P0C0L7)]
* [http://bioinf.cs.ucl.ac.uk/psipred/ PSI-PRED]
* [http://spock.genes.nig.ac.jp/~genome/cgi-bin/gkeytext.pl.cgi?A&QUERY1=P0C0L7 Genomes TO Protein (P0C0L7)] (GTOP)
* [http://www.ebi.ac.uk/msd-srv/pqs/ Protein Quaternary Structure] (PQS)
* [http://spock.genes.nig.ac.jp/~genome/search.html Genomes TO Protein] (GTOP)


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!|Resource type!!Source!!Notes/Reference
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{|   id="T46ab1d5dccde5"  class=" tableEdit Gene_accessions_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="T46ab1d5dccde5"  class=" tableEdit Gene_accessions_table"   
|- {{table_heading_style}}
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!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
|-  
|-  
|
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/protein/16131937 GI:16131937]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=PROP-MONOMER EcoCyc:PROP-MONOMER]
[http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]])
|
|
[http://www.ncbi.nlm.nih.gov/protein/NP_418535.1 GeneID:948626]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[[ASAP]]
|
ASAP:ABE-0013462
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[http://www.pir.uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
|
[http://www.uniprot.org/uniprot/P0C0L7 UniProtKB/Swiss-Prot:P0C0L7]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[[EcoCyc]]
|
EcoCyc:EG11612
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[[EcoGene]]
|
EcoGene:EG11612
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI (Gene)] ([[NCBI (Gene)|EcoliWiki Page]])
|
|
[[uniprot:P0C0L7|UniProt:P0C0L7]]
[http://www.ncbi.nlm.nih.gov/gene?term=948626 GeneID:948626]
|
|
Escherichia coli str. K-12 substr. MG1655
|-  
|-  
|
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[http://pfam.sanger.ac.uk/ PFAM] ([[PFAM|EcoliWiki Page]])
[[RegulonDB]]
|
|
[http://pfam.sanger.ac.uk/family?acc=PF00083 PFAM:PF00083]
RegulonDB:ECK120001560
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
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[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])
[[EchoBASE]]
|
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_418535 RefSeq:NP_418535]
EchoBASE:EB1569
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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{{BacktoTop}}
{{BacktoTop}}
[[Category:Proteins]]
[[Category:Proteins]]
[[Category:GO:0006561_!_proline biosynthetic process]][[Category:GO:0015292_!_uniporter activity]][[Category:GO:0006970_!_response to osmotic stress]][[Category:Location:plasma membrane]]

Latest revision as of 21:12, 17 February 2015

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

ProP

Synonyms

proline/glycine betaine transporter[1], B4111[2][1], ProP[2][1] , ECK4104, JW4072, b4111

Product description

ProP proline/betaine MFS transporter[2][3]

Proline/betaine permease, minor; osmosensor/osmoregulator[4]

EC number (for enzymes)



Notes

Function

[back to top]


Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0016021

integral to membrane

PMID:10026245[5]

IDA: Inferred from Direct Assay

C

complete

GO:0005886

plasma membrane

PMID:10026245[5]

IDA: Inferred from Direct Assay

C

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0997

C

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-1003

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004736

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005828

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0812

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-9909

C

Seeded from EcoCyc (v14.0)

complete

GO:0055085

transmembrane transport

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005828

P

Seeded from EcoCyc (v14.0)

complete

GO:0055085

transmembrane transport

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005829

P

Seeded from EcoCyc (v14.0)

complete

GO:0006865

amino acid transport

PMID:10026245[5]

IDA: Inferred from Direct Assay

P

complete

GO:0006865

amino acid transport

PMID:10026245[5]

IDA: Inferred from Direct Assay

P

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence


Notes

Proline transporter activity is sensitive to the osmolality of the growth medium. Activation and polar localization depend on cardiolipin content of the membrane.

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

plasma membrane

C-terminus localized in the cytoplasm with 12 predicted transmembrane domains

Daley et al. (2005) [6]

plasma membrane

From EcoCyc[3]

Pole

Fluorescence microscopy PMID:17504273[7] PMID:18326496[8]



Notes

Structure and Physical Properties

[back to top]


Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MLKRKKVKPI TLRDVTIIDD GKLRKAITAA SLGNAMEWFD FGVYGFVAYA LGKVFFPGAD
PSVQMVAALA TFSVPFLIRP LGGLFFGMLG DKYGRQKILA ITIVIMSIST FCIGLIPSYD
TIGIWAPILL LICKMAQGFS VGGEYTGASI FVAEYSPDRK RGFMGSWLDF GSIAGFVLGA
GVVVLISTIV GEANFLDWGW RIPFFIALPL GIIGLYLRHA LEETPAFQQH VDKLEQGDRE
GLQDGPKVSF KEIATKYWRS LLTCIGLVIA TNVTYYMLLT YMPSYLSHNL HYSEDHGVLI
IIAIMIGMLF VQPVMGLLSD RFGRRPFVLL GSVALFVLAI PAFILINSNV IGLIFAGLLM
LAVILNCFTG VMASTLPAMF PTHIRYSALA AAFNISVLVA GLTPTLAAWL VESSQNLMMP
AYYLMVVAVV GLITGVTMKE TANRPLKGAT PAASDIQEAK EILVEHYDNI EQKIDDIDHE
IADLQAKRTR LVQQHPRIDE
Length

500

Mol. Wt

54.848 kDa

pI

7.2 (calculated)

Extinction coefficient

63,830 - 64,330 (calc based on 17 Y, 7 W, and 4 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

25..453

PF00083 Sugar (and other) transporter

PMID:19920124[9]

Domain

454..499

PF08946 Osmosensory transporter coiled coil

PMID:19920124[9]

<motif_map/>

tmhmm.php?gene=proP&show=pic&.jpg
Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=proP taxon=562,83333 </beststructure>

Models

View models at:

Structure figures

Notes

Gene Product Resources

[back to top]


See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference


Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131937

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948626

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013462

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0C0L7

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG11612

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11612

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948626

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001560

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1569

Escherichia coli str. K-12 substr. MG1655


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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 5.3 Racher, KI et al. (1999) Purification and reconstitution of an osmosensor: transporter ProP of Escherichia coli senses and responds to osmotic shifts. Biochemistry 38 1676-84 PubMed
  6. ↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
  7. ↑ Romantsov, T et al. (2007) Cardiolipin promotes polar localization of osmosensory transporter ProP in Escherichia coli. Mol. Microbiol. 64 1455-65 PubMed
  8. ↑ Romantsov, T et al. (2008) Cardiolipin controls the osmotic stress response and the subcellular location of transporter ProP in Escherichia coli. J. Biol. Chem. 283 12314-23 PubMed
  9. ↑ 9.0 9.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

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