ada:Gene Product(s): Difference between revisions
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!align=left {{table heading style}} |Synonyms | !align=left {{table heading style}} |Synonyms | ||
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fused DNA-binding transcriptional dual regulator<ref name='LIB:Riley_2006'/>, O6-methylguanine-DNA methyltransferase<ref name='LIB:Riley_2006'/>, B2213<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, Ada<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/> | ''fused DNA-binding transcriptional dual regulator<ref name='LIB:Riley_2006'/>, O6-methylguanine-DNA methyltransferase<ref name='LIB:Riley_2006'/>, B2213<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, Ada<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/> , ECK2205, JW2201, b2213'' | ||
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!align=left {{table heading style}} |Product description | !align=left {{table heading style}} |Product description | ||
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!|Qualifier!!GO ID!!GO term name!!Reference | !|Qualifier!!GO ID!!GO term name!!Reference!!Evidence Code!!with/from!!Aspect!!Notes!!Status | ||
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complete | complete | ||
|- | |||
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GO:0003908 | |||
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methylated-DNA-(protein)-cysteine S-methyltransferase activity | |||
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PMID:6754717 | |||
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IDA: Inferred from Direct Assay | |||
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F | |||
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| | |||
complete | |||
|- | |||
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GO:0005625 | |||
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soluble fraction | |||
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PMID:6754717 | |||
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IDA: Inferred from Direct Assay | |||
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C | |||
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complete | |||
|- | |||
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GO:0006307 | |||
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DNA dealkylation involved in DNA repair | |||
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PMID:6754717 | |||
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IDA: Inferred from Direct Assay | |||
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P | |||
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complete | |||
|- | |||
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GO:0006307 | |||
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DNA dealkylation involved in DNA repair | |||
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PMID: | |||
282622 | |||
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IDA: Inferred from Direct Assay | |||
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P | |||
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<div class="mw-parser-output"><p>complete | |||
</p></div> | |||
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!|Compartment!!Description!!Evidence!!Source!!Notes | !|Compartment!!Description!!Evidence!!Reference/Source!!Notes | ||
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!align=left {{table heading style}} |Name | |||
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!|Type!!Residues!!Description!!Notes!!References | !|Type!!Residues!!Description!!Notes!!References | ||
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[http://pfam.janelia.org/family/PF02805 PF02805 Metal binding domain of Ada] | [http://pfam.janelia.org/family/PF02805 PF02805 Metal binding domain of Ada] | ||
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PMID:19920124 | |||
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[http://pfam.janelia.org/family/PF00165 PF00165 Bacterial regulatory helix-turn-helix proteins, AraC family] | [http://pfam.janelia.org/family/PF00165 PF00165 Bacterial regulatory helix-turn-helix proteins, AraC family] | ||
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PMID:19920124 | |||
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[http://pfam.janelia.org/family/PF00165 PF00165 Bacterial regulatory helix-turn-helix proteins, AraC family] | [http://pfam.janelia.org/family/PF00165 PF00165 Bacterial regulatory helix-turn-helix proteins, AraC family] | ||
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PMID:19920124 | |||
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[http://pfam.janelia.org/family/PF01035 PF01035 6-O-methylguanine DNA methyltransferase, DNA binding domain] | [http://pfam.janelia.org/family/PF01035 PF01035 6-O-methylguanine DNA methyltransferase, DNA binding domain] | ||
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PMID:19920124 | |||
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[http://pfam.janelia.org/family/PF02870 PF02870 6-O-methylguanine DNA methyltransferase, ribonuclease-like domain] | [http://pfam.janelia.org/family/PF02870 PF02870 6-O-methylguanine DNA methyltransferase, ribonuclease-like domain] | ||
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PMID:19920124 | |||
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View models at: | View models at: | ||
* [http:// | * [http://www.proteinmodelportal.org/query/uniprot/P06134 Protein Model Portal] | ||
* [http://bioinf.cs.ucl.ac.uk/genome3d/read?uniprot_id=P06134 PSI-PRED (P06134)] | |||
* [http://bioinf.cs.ucl.ac.uk/ | * [http://spock.genes.nig.ac.jp/~genome/cgi-bin/gkeytext.pl.cgi?A&QUERY1=P06134 Genomes TO Protein (P06134)] (GTOP) | ||
* [http://spock.genes.nig.ac.jp/~genome/ | |||
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!|Database!!Accession!!Notes | !|Database!!Accession!!Notes | ||
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[http:// | [http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]]) | ||
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[http://www.ncbi.nlm.nih.gov/protein/16130150 GI:16130150] | |||
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Escherichia coli str. K-12 substr. MG1655 | |||
|- | |||
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[http:// | [http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]]) | ||
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[http://www.ncbi.nlm.nih.gov/protein/NP_416717.1 GeneID:946710] | |||
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Escherichia coli str. K-12 substr. MG1655 | |||
|- | |||
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[[ASAP]] | |||
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ASAP:ABE-0007311 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |||
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[http://www.pir.uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]]) | |||
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[http://www.uniprot.org/uniprot/P06134 UniProtKB/Swiss-Prot:P06134] | |||
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Escherichia coli str. K-12 substr. MG1655 | |||
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[[EcoCyc]] | |||
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EcoCyc:EG10029 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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[[EcoGene]] | |||
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EcoGene:EG10029 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
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[http:// | [http://www.ncbi.nlm.nih.gov/gene NCBI (Gene)] ([[NCBI (Gene)|EcoliWiki Page]]) | ||
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[ | [http://www.ncbi.nlm.nih.gov/gene?term=946710 GeneID:946710] | ||
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Escherichia coli str. K-12 substr. MG1655 | |||
|- | |- | ||
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[[RegulonDB]] | |||
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RegulonDB:ECK120000026 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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[[EchoBASE]] | |||
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EchoBASE:EB0028 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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[[Category:Proteins]] | [[Category:Proteins]] | ||
Latest revision as of 19:15, 6 June 2024
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
Ada |
|---|---|
| Synonyms |
fused DNA-binding transcriptional dual regulator[1], O6-methylguanine-DNA methyltransferase[1], B2213[2][1], Ada[2][1] , ECK2205, JW2201, b2213 |
| Product description |
Ada transcriptional dual regulator[2][3] O6-methylguanine-DNA methyltransferase, transcription factor; inducible; DNA repair against methylating and alkylating agents; binds Zn(II)[4] |
| EC number (for enzymes) | |
| edit table |
Notes
Function
Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003677 |
DNA binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003700 |
transcription factor activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003824 |
catalytic activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003908 |
methylated-DNA-(protein)-cysteine S-methyltransferase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003908 |
methylated-DNA-(protein)-cysteine S-methyltransferase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003908 |
methylated-DNA-(protein)-cysteine S-methyltransferase activity |
GOA:spec |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005622 |
intracellular |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006281 |
DNA repair |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006281 |
DNA repair |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006281 |
DNA repair |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006281 |
DNA repair |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006281 |
DNA repair |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006350 |
transcription |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006355 |
regulation of transcription, DNA-dependent |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006355 |
regulation of transcription, DNA-dependent |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006974 |
response to DNA damage stimulus |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0008270 |
zinc ion binding |
IDA: Inferred from Direct Assay |
F |
N-terminal domain binds Zn |
complete | |||
| GO:0008168 |
methyltransferase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0008168 |
methyltransferase activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0008270 |
zinc ion binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0008270 |
zinc ion binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0008270 |
zinc ion binding |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc (v14.0) |
complete | |||
| GO:0016740 |
transferase activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0043565 |
sequence-specific DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003908 |
methylated-DNA-(protein)-cysteine S-methyltransferase activity |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0005625 |
soluble fraction |
IDA: Inferred from Direct Assay |
C |
complete | ||||
| GO:0006307 |
DNA dealkylation involved in DNA repair |
IDA: Inferred from Direct Assay |
P |
complete | ||||
| GO:0006307 |
DNA dealkylation involved in DNA repair |
IDA: Inferred from Direct Assay |
P |
complete | ||||
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
|
Protein |
rplB |
Experiment(s):EBI-1142195 | ||
|
Protein |
rpsC |
Experiment(s):EBI-1142195 | ||
|
Protein |
moeB |
Experiment(s):EBI-1142195 | ||
|
Protein |
fur |
Experiment(s):EBI-1142195 | ||
|
Protein |
rpsG |
Experiment(s):EBI-1142195 | ||
|
Protein |
ybiA |
Experiment(s):EBI-1142195 | ||
| edit table |
Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Reference/Source | Notes |
|---|---|---|---|---|
|
membrane |
From EcoCyc[3] |
| ||
| edit table |
Notes
Structure and Physical Properties
Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Name | |
|---|---|
| Sequence |
MKKATCLTDD QRWQSVLARD PNADGEFVFA VRTTGIFCRP SCRARHALRE NVSFYANASE ALAAGFRPCK RCQPEKANAQ QHRLDKITHA CRLLEQETPV TLEALADQVA MSPFHLHRLF KATTGMTPKA WQQAWRARRL RESLAKGESV TTSILNAGFP DSSSYYRKAD ETLGMTAKQF RHGGENLAVR YALADCELGR CLVAESERGI CAILLGDDDA TLISELQQMF PAADNAPADL MFQQHVREVI ASLNQRDTPL TLPLDIRGTA FQQQVWQALR TIPCGETVSY QQLANAIGKP KAVRAVASAC AANKLAIIIP CHRVVRGDGT LSGYRWGVSR KAQLLRREAE NEER |
| Length |
354 |
| Mol. Wt |
39.323 kDa |
| pI |
8.8 (calculated) |
| Extinction coefficient |
36,440 - 37,940 (calc based on 6 Y, 5 W, and 12 C residues) |
| edit table |
Domains/Motifs/Modification Sites
|
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
|
Structure
|
Structure figures
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
| Resource type | Source | Notes/Reference |
|---|---|---|
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 Myers, LC et al. (1992) Zinc binding by the methylation signaling domain of the Escherichia coli Ada protein. Biochemistry 31 4541-7 PubMed
- ↑ 6.0 6.1 6.2 Demple, B et al. (1982) Repair of alkylated DNA in Escherichia coli. Physical properties of O6-methylguanine-DNA methyltransferase. J. Biol. Chem. 257 13776-80 PubMed
- ↑ Schendel, PF & Robins, PE (1978) Repair of O6-methylguanine in adapted Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 75 6017-20 PubMed
- ↑ 8.0 8.1 8.2 8.3 8.4 8.5 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
- ↑ 9.0 9.1 9.2 9.3 9.4 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed
Categories
- GO:0003677 ! DNA binding
- GO:0003700 ! DNA-binding transcription factor activity
- GO:0003824 ! catalytic activity
- GO:0003908 ! methylated-DNA-(protein)-cysteine S-methyltransferase activity
- GO:0005622 ! intracellular
- GO:0006281 ! DNA repair
- GO:0006350 ! transcription
- GO:0006355 ! regulation of transcription, DNA-templated
- GO:0006974 ! cellular response to DNA damage stimulus
- GO:0008270 ! zinc ion binding
- GO:0008168 ! methyltransferase activity
- GO:0016740 ! transferase activity
- GO:0043565 ! sequence-specific DNA binding
- GO:0005625 ! obsolete soluble fraction
- GO:0006307 ! DNA dealkylation involved in DNA repair
- Proteins