galS:Expression: Difference between revisions

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{{Tab|Quickview|galS}}{{Tab|Gene|galS}}{{Tab|Gene Product(s)|galS}}{{Tab On|Expression|galS}}{{Tab|Evolution|galS}}{{Tab|On One Page|galS}}{{TabsBottom}}
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|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
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</noinclude>[[Category:Txn_unit:galS]]
</noinclude>
==Overview==
===Overview===
{{Expression_overview_placeholder}}
{{Expression_overview_placeholder}}
==Cellular Levels==
{{BacktoTop}}
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!|Molecule!!Organism or Strain!!Value!!Units!!Experimental Conditions!!Assay used!!Notes!!Reference(s)
|-
|
Protein
|
''E. coli'' K-12 MG1655
|
3<sup>a</sup>
|
molecules/cell/generation
|
*Medium: MOPS Complete 
* Temperature (°C):  37
*Doubling time (min):
* *Growth phase: 
*OD:
*other:
|
Ribosome Profiling
|
Low confidence in the sequencing data set.
|
PMID: 24766808
|-
|
Protein
|
''E. coli'' K-12 MG1655
|
8
|
molecules/cell/generation
|
*Medium: MOPS Minimal 
* Temperature (°C):  37
*Doubling time (min):
* *Growth phase: 
*OD:
*other:
|
Ribosome Profiling
|
|
PMID: 24766808
|-
|
Protein
|
''E. coli'' K-12 MG1655
|
0<sup>a</sup>
|
molecules/cell/generation
|
*Medium: MOPS Complete without Methionine 
* Temperature (°C):  37
*Doubling time (min):
* *Growth phase: 
*OD:
*other:
|
Ribosome Profiling
|
Low confidence in the sequencing data set.
|
PMID: 24766808
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===Notes===
==Transcription and Transcriptional Regulation==
==Transcription and Transcriptional Regulation==
{{BacktoTop}}
<protect>
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!Transcription unit(s)
!|Transcription unit(s)
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[[:Category:Txn_unit:galS|galS]]  
[[:Category:Txn_unit:galS|galS]]  
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<!--box uid=421aa90e079fa326b6494f812ad13e79..Y46a04ba6baf4b-->||{{RegulonDBfig|ECK120000358.jpg|GALS}}
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<!--Transcription_notes--></protect>
<!--Transcription_notes--></protect>
===Notes===
===Notes===
Line 39: Line 144:


==Translation and Regulation of Translation==
==Translation and Regulation of Translation==
<protect>{{GBrowseFigureByCoord|2239670|2239710|ecoli|MG1655|Genes_unlabeled+DNA_unlabeled+flip|400|{{Nterm_closeup}} }}
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{{Expression_translation_help}}
<protect><gbrowseImage>
name=NC_000913:2239670..2239710
source=MG1655
flip=1
type=Gene+DNA_+Protein
preset=Nterminus
</gbrowseImage>
{{Nterm_closeup}}
 
<!--Translation_notes--></protect>
<!--Translation_notes--></protect>
===Notes===
{{Expression_translation_placeholder}}
{{Expression_translation_placeholder}}


==Turnover and Regulation of Turnover==
==Turnover and Regulation of Turnover==
<!--Turnover_notes--></protect>
===Notes===
{{Expression_turnover_placeholder}}
{{Expression_turnover_placeholder}}


==Experimental==
==Experimental==
{{BacktoTop}}
<protect>
<protect>
===Mutations Affecting Expression===
<big>'''Mutations Affecting Expression'''</big>
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!Allele Name!!Mutation!!Phenotype!!Reference
!|Allele Name!!Mutation!!Phenotype!!Reference
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===Expression Studies===
 
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<big>'''Expression Studies'''</big>
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|
microarray
|
[[geoprofiles:b2151|GEO Profiles:b2151]] ([[Gene_Expression_Omnibus_%28GEO%29|EcoliWiki Page]])
|
NCBI GEO profiles for ''galS''
|-
|
microarray
|
[http://genexpdb.ou.edu/index.php?query=b2151 GenExpDB:b2151] ([[EcoliGenExpDB|EcoliWiki Page]])
|
Summary of data for galS from multiple microarray studies 
 
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===Expression Resources===
 
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<big>'''Expression Resources'''</big>
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!|Resource Name!!Resource Type!!Description!!Source!!Notes
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|
 
|
GFP Fusion
|
Intergenic region (2239619..2239844) fused to <i>gfpmut2</i>.
|
[http://www.openbiosystems.com/GeneExpression/Non-Mammalian/Bacteria/EcoliPromoters/ OpenBioSystems][[Category:Genes in OpenBioSystems with Promoter Fusions]]
|
GFP fusion described in Zaslaver, <i>et al.</i><br />Plate:109-AZ03; Well:B4<ref name="PMID: 16862137" />
 
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<protect><!--Expression_experimental_notes--></protect>
<protect><!--Expression_experimental_notes--></protect>
===Notes===
===Notes===




==Accessions Related to galS Expression==
==Accessions Related to ''galS'' Expression==
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|- {{table_heading_style}}
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!Database!!Accession!!Notes
!|Database!!Accession!!Notes
|-
|-  
|
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
|
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10365 EcoCyc:EG10365] 
EcoCyc:EG10365
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|-  
|
|
[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
[[EchoBASE]]
|
|
[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB0360 EchoBASE:EB0360] 
EchoBASE:EB0360
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|-  
|
|
[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])  
[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])  
|
|
[http://chase.ou.edu/macro/searchlink.php?bnum=b2151 EcoliGenExpDB:b2151]
[http://chase.ou.edu/macro/searchlink.php?bnum=b2151 EcoliGenExpDB:b2151]
|
|
   
   
|-
|-
|
[[EcoGene]]
|
EcoGene:EG10365
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[[RegulonDB]]
|
RegulonDB:ECK120000358
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|
|
[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
[[ASAP]]
|
|
[http://ecogene.org/geneinfo.php?eg_id=EG10365 EcoGene:EG10365] 
ASAP:ABE-0007110
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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<protect><!--Accessions_notes--></protect>
<protect><!--Accessions_notes--></protect>
===Notes===
===Notes===
{{EXPRESSION_ACCESSIONS_NOTES}}
 


==Links==
==Links==
{{BacktoTop}}
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!|Name!!URL!!Comments
 
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==References==
==References==
{{BacktoTop}}
{{RefHelp}}
{{RefHelp}}
<references/>
<references />
__NOTOC__
__NOTOC__
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===Categories===
{{BacktoTop}}
[[Category:Txn_unit:galS]]

Latest revision as of 19:37, 21 July 2015

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Overview Transcription Translation Turnover Experimental Links References Suggestions

Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

[back to top]


See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

3a

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID:24766808[1]

Protein

E. coli K-12 MG1655

8

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID:24766808[1]

Protein

E. coli K-12 MG1655

0a

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID:24766808[1]

edit table

Notes

Transcription and Transcriptional Regulation

[back to top]


See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

galS

edit table
Figure courtesy of RegulonDB


Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<gbrowseImage> name=NC_000913:2239670..2239710 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.


Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

[back to top]


Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
edit table


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b2151 (EcoliWiki Page)

NCBI GEO profiles for galS

microarray

GenExpDB:b2151 (EcoliWiki Page)

Summary of data for galS from multiple microarray studies

edit table


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (2239619..2239844) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ03; Well:B4[2]

edit table

<protect>

Notes

Accessions Related to galS Expression

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10365

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0360

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2151

EcoGene

EcoGene:EG10365

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000358

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007110

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 Li, GW et al. (2014) Quantifying absolute protein synthesis rates reveals principles underlying allocation of cellular resources. Cell 157 624-35 PubMed
  2. ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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Retrieved from "https://ecoliwiki.org/colipedia/index.php?title=galS:Expression&oldid=1501145"