rng:Gene Product(s): Difference between revisions

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!align=left {{table heading style}} |Synonyms
!align=left {{table heading style}} |Synonyms
||
||
ribonuclease G<ref name='LIB:Riley_2006'/>, B3247<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, AdhR<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, Rng<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, YhdF<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, CafA<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>  
''ribonuclease G<ref name='LIB:Riley_2006'/>, B3247<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, AdhR<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, Rng<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, YhdF<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, CafA<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/> , adhR, cafA, ECK3235, JW3216, yhdF, b3247''
|-
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!align=left {{table heading style}} |Product description
!align=left {{table heading style}} |Product description
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* [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4 3.1.4]<ref name='LIB:Riley_2006'/>
* [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4 3.1.4]<ref name='LIB:Riley_2006'/>


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****************************************************************************************** -->{{Product_GO_help}}
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="K46aadb6d0ca24"  class=" tableEdit GO_table_product dataTable" 
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
!|Qualifier!!GO ID!!GO term name!!Reference!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-  
|-  
|
|


|
|
GO:0008996
GO:0003723
|
|
ribonuclease G activity
RNA binding
|
|
PMID:10722715
GOA:interpro<br />GO_REF:0000002
|
|
IDA: Inferred from Direct Assay
IEA: Inferred from Electronic Annotation
|
|
 
InterPro:IPR003029
|
|
F
F
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
Line 103: Line 103:


|
|
GO:0006364
GO:0004519
|
|
rRNA processing
endonuclease activity
|
PMID:10362534
|
IMP: Inferred from Mutant Phenotype
|
 
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|
 
|
GO:0051301
|
cell division
|
|
GO_REF:0000004
GOA:spkw<br />SP_KW:GO_REF:0000004
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
SP_KW:KW-0132
SP_KW:KW-0255
|
P
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
|
complete
|-
|
 
|
GO:0016787
|
hydrolase activity
|
GO_REF:0000004
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0378
|
|
F
F
|
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
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|
|
GO:0007049
GO:0005737
|
|
cell cycle
cytoplasm
|
GO_REF:0000004
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0131
|
P
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
|
complete
|-
|
 
|
|
GO:0006396
GOA:interpro<br />GO_REF:0000002
|
RNA processing
|
GO_REF:0000002
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
InterPro:IPR004659
InterPro:IPR004659
|
P
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
|
complete
|-
|
|
GO:0005856
|
cytoskeleton
|
GO_REF:0000004
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0206
|
|
C
C
|
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
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|
|
F
F
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
|
complete
|-
|
|


|
GO:0004540
|
ribonuclease activity
|
GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR004659
|
F
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
|
|
complete  
complete  
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|
|
GO:0004519
GO:0005737
|
|
endonuclease activity
cytoplasm
|
|
GO_REF:0000004
GOA:spkw<br />SP_KW:GO_REF:0000004
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
SP_KW:KW-0255
SP_KW:KW-0963
|
|
F
C
|
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
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|
|
P
P
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
|
complete
|-
|
|


|
GO:0003723
|
RNA binding
|
GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR003029
|
F
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
|
|
complete  
complete  
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|
|
GO:0005515
GO:0005856
|
|
protein binding
cytoskeleton
|
PMID:15690043
|
IPI: Inferred from Physical Interaction
|
UniProtKB:P0A7V8
|
F
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
|
complete
|-
|
 
|
GO:0003723
|
RNA binding
|
|
GO_REF:0000002
GOA:spkw<br />SP_KW:GO_REF:0000004
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
InterPro:IPR003029
SP_KW:KW-0206
|
F
|
Seeded from EcoCyc
|
complete
|-
|
 
|
GO:0004518
|
nuclease activity
|
GO_REF:0000004
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0540
|
F
|
Seeded from EcoCyc
|
complete
|-
|
 
|
GO:0004519
|
endonuclease activity
|
GO_REF:0000004
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0255
|
F
|
Seeded from EcoCyc
|
complete
|-
|
 
|
GO:0006364
|
rRNA processing
|
PMID:10362534
|
IMP: Inferred from Mutant Phenotype
|
 
|
P
|
Seeded from EcoCyc
|
complete
|-
|
 
|
GO:0005737
|
cytoplasm
|
GO_REF:0000002<br />GO_REF:0000004
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR004659<br />SP_KW:KW-0963
|
|
C
C
|
|
Seeded from EcoCyc
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
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cytoskeleton
cytoskeleton
|
|
GO_REF:0000004
GO_REF:0000023
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
SP_KW:KW-0206
SP_SL:SL-0090
|
|
C
C
|
|
Seeded from EcoCyc
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0006396
|
RNA processing
|
GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR004659
|
P
|
Seeded from EcoCyc
|
|
complete  
complete  
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cell cycle
cell cycle
|
|
GO_REF:0000004
GOA:spkw<br />SP_KW:GO_REF:0000004
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
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P
P
|
|
Seeded from EcoCyc
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
|-  
|-  
|
|
NOT
|
GO:0009451
|
RNA modification
|
|
|
|
P
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.


I think this annotation is incorrect.  RNA modification is The covalent alteration of one or more nucleotides within an RNA molecule to produce an RNA molecule with a sequence that differs from that coded genetically." Rng processes 16S RNA, but I could find no evidence that it modifies the sequence.
|
|
required field missing
GO:0051301
|-
|
|
cell division
|
|
GO:0006401
GOA:spkw<br />SP_KW:GO_REF:0000004
|
|
RNA catabolic process
IEA: Inferred from Electronic Annotation
|
|
SP_KW:KW-0132
|
|
|
P
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
 
I think this annotation is incorrect because processing by Rng is involved in the synthesis of functional ribosomes not the turnover of ribosomes (DAS, TAMU).
|
required field missing
|-  
|
NOT
|
GO:0000910
|
cytokinesis
|
|
|
|
P
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
 
Overproduction of CafA causes a cell division defect: chains of cells and minicells form when expression of ''cafA'' from a multicopy plasmid is induced by the addition of IPTG <ref name= PMID:8300545/>.  Long axial filaments were also observed to form. (DAS, TAMU)
|
required field missing
|-
|
|
GO:0008996
|
ribonuclease G activity
|
PMID:10722715
|
IDA: Inferred from Direct Assay
|
 
|
F
|
 
|
complete
|-
|
|
GO:0006364
|
rRNA processing
|
PMID:10362534
|
IMP: Inferred from Mutant Phenotype
|
 
|
|
P
P
|
|
 
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0008996
|
ribonuclease G activity
|
PMID:10722715
|
IDA: Inferred from Direct Assay
|
 
|
F
|
Seeded from EcoCyc
|
|
complete  
complete  


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!|Partner Type!!Partner!!Notes!!References!!Evidence
!|Partner Type!!Partner!!Notes!!References!!Evidence
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|
|
Experiment(s):EBI-895082<br />  
Experiment(s):EBI-895082<br />  
|-
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Protein
|
rpsT
|


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PMID:19402753
|
LCMS(ID Probability):99.6
|-
|
Protein
|
rpsU
|
 
|
PMID:19402753
|
LCMS(ID Probability):99.6
|-
|
Protein
|
rpe
|
 
|
PMID:19402753
|
LCMS(ID Probability):99.2
|-
|
Protein
|
rpsS
|
 
|
PMID:19402753
|
LCMS(ID Probability):99.6
|-
|
Protein
|
eno
|
 
|
PMID:19402753
|
LCMS(ID Probability):99.6
|-
|
Protein
|
rpsM
|
 
|
PMID:19402753
|
LCMS(ID Probability):99.6
 
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!|Compartment!!Description!!Evidence!!Notes
!|Compartment!!Description!!Evidence!!Reference/Source!!Notes
|-  
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From EcoCyc<ref name='LIB:EcoCyc11.1'/>   
From EcoCyc<ref name='LIB:EcoCyc11.1'/>  
|
   


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!align=left {{table heading style}} |Name
||


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!align=left {{table heading style}} |Mol. Wt
!align=left {{table heading style}} |Mol. Wt
||
||
55.36 kDa (calc) <ref name='LIB:EcoCyc10.6'/>
56.063 kDa
|-
|-
!align=left {{table heading style}} |pI
!align=left {{table heading style}} |pI
||
||
6.0 (calculated)
|-
|-
!align=left {{table heading style}} |Extinction coefficient
!align=left {{table heading style}} |Extinction coefficient
||
||
27850 - 28600 (calc based on 15 Y, 1 W, and 6 C residues)  
27,850 - 28,600 (calc based on 15 Y, 1 W, and 6 C residues)


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!|Type!!Residues!!Description!!Notes
!|Type!!Residues!!Description!!Notes!!References
|-
|
Initiator Methionine
|
1
|
Removed
|
[http://www.uniprot.org/uniprot/P0A9J0#section_features UniProt:P0A9J0]
|
|-  
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|
Domain
Domain
|
|
39 - 128
35..122
|
|
[[interpro:IPR003029|S1 motif]]
[http://pfam.janelia.org/family/PF00575 PF00575  S1 RNA binding domain]
|
|
[http://www.uniprot.org/uniprot/P0A9J0#section_features UniProt:P0A9J0]


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PMID:19920124
|-
|
Domain
|
124..394
|
[http://pfam.janelia.org/family/PF10150 PF10150  Ribonuclease E/G family]
|
 
|
PMID:19920124
 
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</table>
</table>


<span id="Structure"></span><big>'''Structure'''</big>
<table><tr valign='top'><td>
<span id='Structure'></span><big>'''Structure'''</big><br />
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|-
|-
!align=left {{table heading style}} |Structures
!align=left {{table heading style}} |Structures
||
||
<beststructure>rng
<beststructure>
562</beststructure>
gene=rng
 
taxon=562,83333
</beststructure>
|-
|-
!align=left {{table heading style}} |Models
!align=left {{table heading style}} |Models
||
||
View models at:
View models at:
* [http://dragon.bio.purdue.edu/ecolpredict2/localsearch/cgi-bin/search3.cgi?q=rng&sub=keywordSearc EcoliPredict]
* [http://www.proteinmodelportal.org/query/uniprot/P0A9J0 Protein Model Portal]
* [http://modbase.compbio.ucsf.edu/modbase-cgi/model_search.cgi?searchmode=default&displaymode=moddetail&searchproperties=ALL&searchvalue=rng&organism=562&organismtext= ModBase]
* [http://bioinf.cs.ucl.ac.uk/genome3d/read?uniprot_id=P0A9J0 PSI-PRED (P0A9J0)]
* [http://bioinf.cs.ucl.ac.uk/psipred/ PSI-PRED]
* [http://spock.genes.nig.ac.jp/~genome/cgi-bin/gkeytext.pl.cgi?A&QUERY1=P0A9J0 Genomes TO Protein (P0A9J0)] (GTOP)
* [http://www.ebi.ac.uk/msd-srv/pqs/ Protein Quaternary Structure] (PQS)
* [http://spock.genes.nig.ac.jp/~genome/search.html Genomes TO Protein] (GTOP)


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<!--Structure_notes--></protect>
</td><td></protect>
==== Structure figures ====
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
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===Notes===
===Notes===


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!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
|-  
|-  
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/protein/90111563 GI:90111563]
|
Escherichia coli str. K-12 substr. MG1655
|-
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[http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]])
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[http://www.ncbi.nlm.nih.gov/protein/NP_417713.2 GeneID:947744]
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Escherichia coli str. K-12 substr. MG1655
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[[ASAP]]
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ASAP:ABE-0010651
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
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[http://www.pir.uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
|
[http://www.uniprot.org/uniprot/P0A9J0 UniProtKB/Swiss-Prot:P0A9J0]
|
Escherichia coli str. K-12 substr. MG1655
|-
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[[EcoCyc]]
|
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG11299-MONOMER EcoCyc:EG11299-MONOMER]
EcoCyc:EG11299
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
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[[EcoGene]]
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EcoGene:EG11299
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
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[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI (Gene)] ([[NCBI (Gene)|EcoliWiki Page]])
|
|
[[uniprot:P0A9J0|UniProt:P0A9J0]]
[http://www.ncbi.nlm.nih.gov/gene?term=947744 GeneID:947744]
|
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Escherichia coli str. K-12 substr. MG1655
|-  
|-  
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[http://pfam.sanger.ac.uk/ PFAM] ([[PFAM|EcoliWiki Page]])
[[RegulonDB]]
|
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[http://pfam.sanger.ac.uk/family?acc=PF00575 PFAM:PF00575]
RegulonDB:ECK120001272
|
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
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[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])
[[EchoBASE]]
|
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[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_417713 RefSeq:NP_417713]
EchoBASE:EB1276
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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==References==
==References==
{{BacktoTop}}
{{BacktoTop}}
 
{{RefHelp}}
<references/>
<references />
__NOTOC__
__NOTOC__
</noinclude>
===Categories===
===Categories===
{{BacktoTop}}
{{BacktoTop}}
[[Category:Proteins]]
[[Category:Proteins]]
[[Category:GO:0005737_!_cytoplasm]][[Category:GO:0009451_!_RNA modification]][[Category:GO:0016072_!_rRNA metabolic process]][[Category:GO:0006401_!_RNA catabolic process]][[Category:GO:0000910_!_cytokinesis]][[Category:Complex:ribonuclease G (RNAse G)]][[Category:Location:cytoplasm]]

Latest revision as of 21:01, 17 February 2015

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

Rng

Synonyms

ribonuclease G[1], B3247[2][1], AdhR[2][1], Rng[2][1], YhdF[2][1], CafA[2][1] , adhR, cafA, ECK3235, JW3216, yhdF, b3247

Product description

ribonuclease G (RNAse G)[2][3];

Component of ribonuclease G (RNAse G)[3]

Endoribonuclease G involved in 16S rRNA processing; division and growth, overexpression forms minicells and chains with long axial structures[4]

EC number (for enzymes)


Notes

Function

[back to top]


Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0003723

RNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003029

F

Seeded from EcoCyc (v14.0)

complete

GO:0004519

endonuclease activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0255

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004659

C

Seeded from EcoCyc (v14.0)

complete

GO:0008996

ribonuclease G activity

PMID:10722715[5]

IDA: Inferred from Direct Assay

F

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0006364

rRNA processing

PMID:10362534[6]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0005856

cytoskeleton

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0206

C

Seeded from EcoCyc (v14.0)

complete

GO:0005856

cytoskeleton

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0090

C

Seeded from EcoCyc (v14.0)

complete

GO:0007049

cell cycle

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0131

P

Seeded from EcoCyc (v14.0)

complete

GO:0051301

cell division

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0132

P

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of ribonuclease G (RNAse G)

could be indirect

Protein

rpsD

PMID:15690043[7]

Experiment(s):EBI-890107

Protein

rplB

PMID:16606699[8]

Experiment(s):EBI-1145286

Protein

rpsI

PMID:16606699[8]

Experiment(s):EBI-1145286

Protein

rpsC

PMID:16606699[8]

Experiment(s):EBI-1145286

Protein

rpsB

PMID:16606699[8]

Experiment(s):EBI-1145286

Protein

rplQ

PMID:16606699[8]

Experiment(s):EBI-1145286

Protein

racC

PMID:16606699[8]

Experiment(s):EBI-1145286

Protein

dnaK

PMID:15690043[7]

Experiment(s):EBI-895082

Protein

eno

PMID:15690043[7]

Experiment(s):EBI-895082

Protein

rpe

PMID:15690043[7]

Experiment(s):EBI-895082

Protein

rpsE

PMID:15690043[7]

Experiment(s):EBI-895082

Protein

rpsI

PMID:15690043[7]

Experiment(s):EBI-895082

Protein

rpsM

PMID:15690043[7]

Experiment(s):EBI-895082

Protein

rpsO

PMID:15690043[7]

Experiment(s):EBI-895082

Protein

rpsS

PMID:15690043[7]

Experiment(s):EBI-895082

Protein

rpsT

PMID:15690043[7]

Experiment(s):EBI-895082

Protein

rpsU

PMID:15690043[7]

Experiment(s):EBI-895082

Protein

rpsT

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

rpsU

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

rpe

PMID:19402753[9]

LCMS(ID Probability):99.2

Protein

rpsS

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

eno

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

rpsM

PMID:19402753[9]

LCMS(ID Probability):99.6


Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

cytoplasm

From EcoCyc[3]



Notes

Structure and Physical Properties

[back to top]


Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MRKGINMTAE LLVNVTPSET RVAYIDGGIL QEIHIEREAR RGIVGNIYKG RVSRVLPGMQ
AAFVDIGLDK AAFLHASDIM PHTECVAGEE QKQFTVRDIS ELVRQGQDLM VQVVKDPLGT
KGARLTTDIT LPSRYLVFMP GASHVGVSQR IESESERERL KKVVAEYCDE QGGFIIRTAA
EGVGEAELAS DAAYLKRVWT KVMERKKRPQ TRYQLYGELA LAQRVLRDFA DAELDRIRVD
SRLTYEALLE FTSEYIPEMT SKLEHYTGRQ PIFDLFDVEN EIQRALERKV ELKSGGYLII
DQTEAMTTVD INTGAFVGHR NLDDTIFNTN IEATQAIARQ LRLRNLGGII IIDFIDMNNE
DHRRRVLHSL EQALSKDRVK TSVNGFSALG LVEMTRKRTR ESIEHVLCNE CPTCHGRGTV
KTVETVCYEI MREIVRVHHA YDSDRFLVYA SPAVAEALKG EESHSLAEVE IFVGKQVKVQ
IEPLYNQEQF DVVMM
Length

495

Mol. Wt

56.063 kDa

pI

6.0 (calculated)

Extinction coefficient

27,850 - 28,600 (calc based on 15 Y, 1 W, and 6 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P0A9J0

Domain

35..122

PF00575 S1 RNA binding domain

PMID:19920124[10]

Domain

124..394

PF10150 Ribonuclease E/G family

PMID:19920124[10]

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=rng taxon=562,83333 </beststructure>

Models

View models at:

Structure figures

Notes

Gene Product Resources

[back to top]


See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference


Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:90111563

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947744

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010651

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A9J0

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG11299

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11299

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947744

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001272

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1276

Escherichia coli str. K-12 substr. MG1655


Notes

Links

[back to top]




References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ Tock, MR et al. (2000) The CafA protein required for the 5'-maturation of 16 S rRNA is a 5'-end-dependent ribonuclease that has context-dependent broad sequence specificity. J. Biol. Chem. 275 8726-32 PubMed
  6. ↑ Wachi, M et al. (1999) Escherichia coli cafA gene encodes a novel RNase, designated as RNase G, involved in processing of the 5' end of 16S rRNA. Biochem. Biophys. Res. Commun. 259 483-8 PubMed
  7. ↑ 7.00 7.01 7.02 7.03 7.04 7.05 7.06 7.07 7.08 7.09 7.10 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  8. ↑ 8.0 8.1 8.2 8.3 8.4 8.5 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  9. ↑ 9.0 9.1 9.2 9.3 9.4 9.5 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  10. ↑ 10.0 10.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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