appA:Gene Product(s): Difference between revisions

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touch the Product_localization_table table.
touch table
 
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!align=left {{table heading style}} |Synonyms
!align=left {{table heading style}} |Synonyms
||
||
phosphoanhydride phosphorylase<ref name='LIB:Riley_2006'/>, B0980<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, AppA<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>  
''phosphoanhydride phosphorylase<ref name='LIB:Riley_2006'/>, B0980<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, AppA<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/> , ECK0971, JW0963, b0980''
|-
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!align=left {{table heading style}} |Product description
!align=left {{table heading style}} |Product description
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* [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2]<ref name='LIB:Riley_2006'/>
* [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2]<ref name='LIB:Riley_2006'/>


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****************************************************************************************** -->{{Product_GO_help}}
****************************************************************************************** -->{{Product_GO_help}}
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="O46aa4fb38a97d"  class=" tableEdit GO_table_product dataTable"   
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
!|Qualifier!!GO ID!!GO term name!!Reference!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-  
|-  
|
|


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GO:0042597
GO:0030288
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periplasmic space
outer membrane-bounded periplasmic space
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GO_REF:0000004
 
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IEA: Inferred from Electronic Annotation
 
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SP_KW:KW-0574
 
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C
C
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from Riley et  al 2006 <ref name='LIB:Riley_2006'/>.
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complete
Missing: evidence, reference
|-  
|-  
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catalytic activity
catalytic activity
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GO_REF:0000004
GOA:spkw<br />SP_KW:GO_REF:0000004
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IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
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F
F
|
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
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acid phosphatase activity
acid phosphatase activity
|
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GO_REF:0000002<br />GO_REF:0000003
GOA:interpro<br />GO_REF:0000002
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IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
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InterPro:IPR000560<br />EC:3.1.3.2
InterPro:IPR000560
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F
F
|
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
Seeded from [[EcoCyc]] (v14.0)
|
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complete  
complete  
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GO:0008707
GO:0003993
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4-phytase activity
acid phosphatase activity
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GO_REF:0000003
GOA:spec<br />GO_REF:0000003
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IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
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EC:3.1.3.26
EC:3.1.3.2
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F
F
|
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
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GO:0016787
GO:0008707
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hydrolase activity
4-phytase activity
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GO_REF:0000004
GOA:spec<br />GO_REF:0000003
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IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
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SP_KW:KW-0378
EC:3.1.3.26
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F
F
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|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
|-  
|-  
|
|
 
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GO:0030288
GO:0016787
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outer membrane-bounded periplasmic space
hydrolase activity
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GOA:spkw<br />SP_KW:GO_REF:0000004
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IEA: Inferred from Electronic Annotation
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SP_KW:KW-0378
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C
F
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|
Seeded from Riley et  al 2006 <ref name='LIB:Riley_2006'/>.  
Seeded from [[EcoCyc]] (v14.0)
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required fields missing 
complete
|-  
|-  
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|
 
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GO:0006793
GO:0042597
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phosphorus metabolic process
periplasmic space
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GOA:spkw<br />SP_KW:GO_REF:0000004
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IEA: Inferred from Electronic Annotation
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SP_KW:KW-0574
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P
C
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Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.  
Seeded from [[EcoCyc]] (v14.0)
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required fields missing 
complete
|-  
|-  
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|
 
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GO:0042594
GO:0042597
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response to starvation
periplasmic space
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GO_REF:0000023
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IEA: Inferred from Electronic Annotation
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SP_SL:SL-0200
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P
C
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|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.  
Seeded from [[EcoCyc]] (v14.0)
|
|
required fields missing 
complete


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*  
*  
****************************************************************************************** -->{{Product_interactions_help}}
****************************************************************************************** -->{{Product_interactions_help}}
{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"   
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|- {{table heading style}}
|- {{table heading style}}
!|Partner Type!!Partner!!Notes!!References!!Evidence
!|Partner Type!!Partner!!Notes!!References!!Evidence
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Experiment(s):EBI-1138534<br />  
Experiment(s):EBI-1138534<br />  


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*  
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****************************************************************************************** -->{{Product_localization_help}}
****************************************************************************************** -->{{Product_localization_help}}
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!|Compartment!!Description!!Evidence!!Source!!Notes
!|Compartment!!Description!!Evidence!!Reference/Source!!Notes
|-  
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From EcoCyc<ref name='LIB:EcoCyc11.1'/>  
From EcoCyc<ref name='LIB:EcoCyc11.1'/>  
|
|-
|
Periplasm
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|
PMID:8387749
|
[http://www.york.ac.uk/res/thomas/Echolocation.cfm?EB_SubLocID=EB0047 EchoLocation:appA]
|
|
   
   


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****************************************************************************************** -->{{Product_physical_properties_help}}
****************************************************************************************** -->{{Product_physical_properties_help}}
{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
{|   id="O46aa4fc1e2c49" class=" tableEdit Product_phys_prop_table"
 
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!align=left {{table heading style}} |Name
||


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49,960 - 50,960 (calc based on 4 Y, 8 W, and 8 C residues)
49,960 - 50,960 (calc based on 4 Y, 8 W, and 8 C residues)


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*  
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****************************************************************************************** -->{{Product_domains_motifs_help}}
****************************************************************************************** -->{{Product_domains_motifs_help}}
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!|Type!!Residues!!Description!!Notes
!|Type!!Residues!!Description!!Notes!!References
|-  
|-  
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[[uniprotmanual:signal|UniProt Manual:Signal Peptides]]
[[uniprotmanual:signal|UniProt Manual:Signal Peptides]]
|
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[http://www.uniprot.org/uniprot/P07102#section_features UniProt:P07102]  
[http://www.uniprot.org/uniprot/P07102#section_features UniProt:P07102]
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|-
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Domain
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29..377
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[http://pfam.janelia.org/family/PF00328 PF00328  Histidine acid phosphatase]
|


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PMID:19920124
 
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*  
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****************************************************************************************** -->{{Product_structure_help}}
****************************************************************************************** -->{{Product_structure_help}}
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||
||
View models at:
View models at:
* [http://dragon.bio.purdue.edu/ecolpredict2/localsearch/cgi-bin/search3.cgi?q=recA EcoliPredict]
* [http://www.proteinmodelportal.org/query/uniprot/P07102 Protein Model Portal]
* [http://modbase.compbio.ucsf.edu/modbase-cgi/model_search.cgi?searchmode=default&displaymode=moddetail&searchproperties=ALL&searchvalue=recA&organism=562&organismtext= ModBase]
* [http://bioinf.cs.ucl.ac.uk/genome3d/read?uniprot_id=P07102 PSI-PRED (P07102)]
* [http://bioinf.cs.ucl.ac.uk/psipred/ PSI-PRED]
* [http://spock.genes.nig.ac.jp/~genome/cgi-bin/gkeytext.pl.cgi?A&QUERY1=P07102 Genomes TO Protein (P07102)] (GTOP)
* [http://www.ebi.ac.uk/msd-srv/pqs/ Protein Quaternary Structure] (PQS)
* [http://spock.genes.nig.ac.jp/~genome/search.html Genomes TO Protein] (GTOP)


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*  
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****************************************************************************************** -->{{Product_resources_help}}
****************************************************************************************** -->{{Product_resources_help}}
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|- {{table_heading_style}}
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!|Resource type!!Source!!Notes/Reference
!|Resource type!!Source!!Notes/Reference


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*  
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****************************************************************************************** -->{{Gene_accessions_help}}
****************************************************************************************** -->{{Gene_accessions_help}}
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|- {{table_heading_style}}
|- {{table_heading_style}}
!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
|-  
|-  
|
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]])
|
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=APPA-MONOMER EcoCyc:APPA-MONOMER]
[http://www.ncbi.nlm.nih.gov/protein/16128946 GI:16128946]
|
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/protein/NP_415500.1 GeneID:946206]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[[ASAP]]
|
ASAP:ABE-0003305
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[http://www.pir.uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
|
[http://www.uniprot.org/uniprot/P07102 UniProtKB/Swiss-Prot:P07102]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[[EcoCyc]]
|
EcoCyc:EG10049
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[[EcoGene]]
|
EcoGene:EG10049
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI (Gene)] ([[NCBI (Gene)|EcoliWiki Page]])
|
|
[[uniprot:P07102|UniProt:P07102]]
[http://www.ncbi.nlm.nih.gov/gene?term=946206 GeneID:946206]
|
|
Escherichia coli str. K-12 substr. MG1655
|-  
|-  
|
|
[http://pfam.sanger.ac.uk/ PFAM] ([[PFAM|EcoliWiki Page]])
[[RegulonDB]]
|
|
[http://pfam.sanger.ac.uk/family?acc=PF00328 PFAM:PF00328]
RegulonDB:ECK120000045
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|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])
[[EchoBASE]]
|
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_415500 RefSeq:NP_415500]
EchoBASE:EB0047
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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!|Name!!URL!!Comments
!|Name!!URL!!Comments


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[[Category:Proteins]]
[[Category:Proteins]]
[[Category:GO:0030288_!_outer membrane-bounded periplasmic space]][[Category:GO:0006793_!_phosphorus metabolic process]][[Category:GO:0042594_!_response to starvation]][[Category:Location:periplasm]][[Category:Products with structures in PDB]]

Latest revision as of 20:32, 17 February 2015

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

AppA

Synonyms

phosphoanhydride phosphorylase[1], B0980[2][1], AppA[2][1] , ECK0971, JW0963, b0980

Product description

AppA[2][3]

Acid phosphatase, pH 2.5, exopolyphosphatase; phytase[4]

EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0030288

outer membrane-bounded periplasmic space

C

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0003824

catalytic activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0511

F

Seeded from EcoCyc (v14.0)

complete

GO:0003993

acid phosphatase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000560

F

Seeded from EcoCyc (v14.0)

complete

GO:0003993

acid phosphatase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:3.1.3.2

F

Seeded from EcoCyc (v14.0)

complete

GO:0008707

4-phytase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:3.1.3.26

F

Seeded from EcoCyc (v14.0)

complete

GO:0016787

hydrolase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0378

F

Seeded from EcoCyc (v14.0)

complete

GO:0042597

periplasmic space

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0574

C

Seeded from EcoCyc (v14.0)

complete

GO:0042597

periplasmic space

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0200

C

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

eaeH

PMID:16606699[5]

Experiment(s):EBI-1138534

Protein

rplB

PMID:16606699[5]

Experiment(s):EBI-1138534
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

periplasm

From EcoCyc[3]

Periplasm

PMID:8387749[6]

EchoLocation:appA


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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MKAILIPFLS LLIPLTPQSA FAQSEPELKL ESVVIVSRHG VRAPTKATQL MQDVTPDAWP
TWPVKLGWLT PRGGELIAYL GHYQRQRLVA DGLLAKKGCP QSGQVAIIAD VDERTRKTGE
AFAAGLAPDC AITVHTQADT SSPDPLFNPL KTGVCQLDNA NVTDAILSRA GGSIADFTGH
RQTAFRELER VLNFPQSNLC LKREKQDESC SLTQALPSEL KVSADNVSLT GAVSLASMLT
EIFLLQQAQG MPEPGWGRIT DSHQWNTLLS LHNAQFYLLQ RTPEVARSRA TPLLDLIKTA
LTPHPPQKQA YGVTLPTSVL FIAGHDTNLA NLGGALELNW TLPGQPDNTP PGGELVFERW
RRLSDNSQWI QVSLVFQTLQ QMRDKTPLSL NTPPGEVKLT LAGCEERNAQ GMCSLAGFTQ
IVNEARIPAC SL
Length

432

Mol. Wt

47.057 kDa

pI

6.7 (calculated)

Extinction coefficient

49,960 - 50,960 (calc based on 4 Y, 8 W, and 8 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

motif

1-22

UniProt Manual:Signal Peptides

UniProt:P07102

Domain

29..377

PF00328 Histidine acid phosphatase

PMID:19920124[7]

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=appA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128946

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946206

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003305

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P07102

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10049

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10049

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946206

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000045

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0047

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  6. ↑ Greiner, R et al. (1993) Purification and characterization of two phytases from Escherichia coli. Arch. Biochem. Biophys. 303 107-13 PubMed
  7. ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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