cynS:Gene Product(s): Difference between revisions

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!align=left {{table heading style}} |Synonyms
!align=left {{table heading style}} |Synonyms
||
||
cyanate aminohydrolase<ref name='LIB:Riley_2006'/>, B0340<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, Cnt<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, CyaN<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, CynS<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, cyanate hydrolase<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, cyanase<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, cyanate C-N-lyase<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>  
''cyanate aminohydrolase<ref name='LIB:Riley_2006'/>, B0340<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, Cnt<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, CyaN<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, CynS<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, cyanate hydrolase<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, cyanase<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, cyanate C-N-lyase<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/> , cnt, ECK0337, JW0331, b0340''
|-
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!align=left {{table heading style}} |Product description
!align=left {{table heading style}} |Product description
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* [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.104 4.2.1.104]<ref name='LIB:Riley_2006'/>
* [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.104 4.2.1.104]<ref name='LIB:Riley_2006'/>


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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="C46aa2990e91ad"  class=" tableEdit GO_table_product dataTable" 
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!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
!|Qualifier!!GO ID!!GO term name!!Reference!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-  
|-  
|
|


|
|
GO:0008824
GO:0005737
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|
cyanate hydratase activity
cytoplasm
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|
GO_REF:0000002<br />GO_REF:0000003<br />GO_REF:0000020
PMID:8083164
|
|
IEA: Inferred from Electronic Annotation
IDA: Inferred from Direct Assay
|
InterPro:IPR003712<br />EC:4.2.1.104<br />HAMAP:MF_00535
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|
|


|
|
GO:0016836
C
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|
hydro-lyase activity
Seeded from Riley et  al 2006 <ref name='LIB:Riley_2006'/>.
|
GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
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InterPro:IPR008076
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
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|
complete  
complete  
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DNA binding
DNA binding
|
|
GO_REF:0000002
GOA:interpro<br />GO_REF:0000002
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IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
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F
F
|
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
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|
|
GO:0005737
GO:0008824
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|
cytoplasm
cyanate hydratase activity
|
|
PMID:8083164
GOA:hamap<br />GO_REF:0000020
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|
IDA: Inferred from Direct Assay
IEA: Inferred from Electronic Annotation
|
|
 
HAMAP:MF_00535
|
|
C
F
|
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
Seeded from [[EcoCyc]] (v14.0)
|
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complete  
complete  
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cyanate hydratase activity
cyanate hydratase activity
|
|
PMID:3651424
GOA:interpro<br />GO_REF:0000002
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|
IDA: Inferred from Direct Assay
IEA: Inferred from Electronic Annotation
|
|
 
InterPro:IPR003712
|
|
F
F
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
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GO:0009440
GO:0008824
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cyanate catabolic process
cyanate hydratase activity
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PMID:3309165
GOA:spec<br />GO_REF:0000003
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|
IMP: Inferred from Mutant Phenotype
IEA: Inferred from Electronic Annotation
|
|
 
EC:4.2.1.104
|
|
P
F
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
|
GO:0005737
|
cytoplasm
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PMID:8083164
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IDA: Inferred from Direct Assay
|
|
C
|
Seeded from Riley et  al 2006 <ref name='LIB:Riley_2006'/>.  
|
|
complete  
complete  
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|
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GO:0008152
GO:0016836
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|
metabolic process
hydro-lyase activity
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|
GO_REF:0000002
GOA:interpro<br />GO_REF:0000002
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
InterPro:IPR008076
InterPro:IPR008076
|
P
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
|
complete
|-
|
|
GO:0009440
|
cyanate catabolic process
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PMID:3309165
|
|
|
P
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
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required field missing
|-
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GO:0006807
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nitrogen compound metabolic process
|
|
|
|
P
|
Seeded from EcoCyc 10.6<ref name='LIB:EcoCyc10.6'/>.
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required fields missing 
|-
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GO:0006805
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xenobiotic metabolic process
|
|
|
|
P
|
Seeded from EcoCyc 10.6<ref name='LIB:EcoCyc10.6'/>.
|
required fields missing 
|-
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GO:0008824
|
cyanate hydratase activity
|
PMID:3651424
|
|
|
|
F
F
|
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
Seeded from [[EcoCyc]] (v14.0)
|
required field missing
|-
|
 
|
GO:0009439
|
cyanate metabolic process
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GO_REF:0000020<br />GO_REF:0000002
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IEA: Inferred from Electronic Annotation
|
HAMAP:MF_00535<br />InterPro:IPR003712
|
P
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
|
|
complete  
complete  


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!|Partner Type!!Partner!!Notes!!References!!Evidence
!|Partner Type!!Partner!!Notes!!References!!Evidence
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Experiment(s):EBI-1136296<br />  
Experiment(s):EBI-1136296<br />  


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!|Compartment!!Description!!Evidence!!Notes
!|Compartment!!Description!!Evidence!!Reference/Source!!Notes


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!align=left {{table heading style}} |Name
||


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VGAKLDLDED SILLLQMIPL RGCIDDRIPT DPTMYRFYEM LQVYGTTLKA LVHEKFGDGI
VGAKLDLDED SILLLQMIPL RGCIDDRIPT DPTMYRFYEM LQVYGTTLKA LVHEKFGDGI
ISAINFKLDV KKVADPEGGE RAVITLDGKY LPTKPF</pre>  
ISAINFKLDV KKVADPEGGE RAVITLDGKY LPTKPF</pre>  
|-
!align=left {{table heading style}} |Length
||
156
|-
|-
!align=left {{table heading style}} |Mol. Wt
!align=left {{table heading style}} |Mol. Wt
||
||
17.05 kDa (calc) <ref name='LIB:EcoCyc10.6'/>
17.049 kDa
|-
|-
!align=left {{table heading style}} |pI
!align=left {{table heading style}} |pI
||
||
5.29<ref name='LIB:EcoCyc10.6'/>
4.8 (calculated)
|-
|-
!align=left {{table heading style}} |Extinction coefficient
!align=left {{table heading style}} |Extinction coefficient
||
||
5960 - 6085 (calc based on 4 Y, 0 W, and 1 C residues)  
5,960 - 6,085 (calc based on 4 Y, 0 W, and 1 C residues)


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<span id="Domains/Motifs/Modification Sites"><big>'''Domains/Motifs/Modification Sites'''</big></span>
<span id="Domains/Motifs/Modification Sites"><big>'''Domains/Motifs/Modification Sites'''</big></span><table>
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|- {{table heading style}}
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!|Type!!Residues!!Description!!Notes
!|Type!!Residues!!Description!!Notes!!References
|-
|
Domain
|
83..156
|
[http://pfam.janelia.org/family/PF02560 PF02560  Cyanate lyase C-terminal domain]
|
 
|
PMID:19920124


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<td>
<!-- motifs column 2-->
<motif_map/>
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<span id="Structure"></span><big>'''Structure'''</big>
<table><tr valign='top'><td>
<span id='Structure'></span><big>'''Structure'''</big><br />
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|-
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!align=left {{table heading style}} |Structures
!align=left {{table heading style}} |Structures
||
||
<beststructure>cynS
<beststructure>
562</beststructure>
gene=cynS
 
taxon=562,83333
</beststructure>
|-
|-
!align=left {{table heading style}} |Models
!align=left {{table heading style}} |Models
||
||
View models at:
View models at:
* [http://dragon.bio.purdue.edu/ecolpredict2/localsearch/cgi-bin/search3.cgi?q=cynS&sub=keywordSearc EcoliPredict]
* [http://www.proteinmodelportal.org/query/uniprot/P00816 Protein Model Portal]
* [http://modbase.compbio.ucsf.edu/modbase-cgi/model_search.cgi?searchmode=default&displaymode=moddetail&searchproperties=ALL&searchvalue=cynS&organism=562&organismtext= ModBase]
* [http://bioinf.cs.ucl.ac.uk/genome3d/read?uniprot_id=P00816 PSI-PRED (P00816)]
* [http://bioinf.cs.ucl.ac.uk/psipred/ PSI-PRED]
* [http://spock.genes.nig.ac.jp/~genome/cgi-bin/gkeytext.pl.cgi?A&QUERY1=P00816 Genomes TO Protein (P00816)] (GTOP)
* [http://www.ebi.ac.uk/msd-srv/pqs/ Protein Quaternary Structure] (PQS)
* [http://spock.genes.nig.ac.jp/~genome/search.html Genomes TO Protein] (GTOP)


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<!--Structure_notes--></protect>
</td><td></protect>
==== Structure figures ====
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
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===Notes===
===Notes===


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!|Resource type!!Source!!Notes/Reference
!|Resource type!!Source!!Notes/Reference


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!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
|-  
|-  
|
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/protein/16128325 GI:16128325]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/protein/NP_414874.1 GeneID:948998]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[[ASAP]]
|
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=CYANLY-MONOMER EcoCyc:CYANLY-MONOMER]
ASAP:ABE-0001173
|
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://www.pir.uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
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[http://www.uniprot.org/uniprot/P00816 UniProtKB/Swiss-Prot:P00816]
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Escherichia coli str. K-12 substr. MG1655
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[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
[[EcoCyc]]
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[[uniprot:P00816|UniProt:P00816]]
EcoCyc:EG10175
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://pfam.sanger.ac.uk/ PFAM] ([[PFAM|EcoliWiki Page]])
[[EcoGene]]
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[http://pfam.sanger.ac.uk/family?acc=PF02560 PFAM:PF02560]
EcoGene:EG10175
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI (Gene)] ([[NCBI (Gene)|EcoliWiki Page]])
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[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_414874 RefSeq:NP_414874]
[http://www.ncbi.nlm.nih.gov/gene?term=948998 GeneID:948998]
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Escherichia coli str. K-12 substr. MG1655
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[http://modbase.compbio.ucsf.edu ModBase] ([[ModBase|EcoliWiki Page]])
[[RegulonDB]]
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[http://salilab.org/modbase/searchbyid?databaseID=P00816 ModBase:P00816]
RegulonDB:ECK120000170
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://www.rcsb.org/pdb PDB] ([[PDB|EcoliWiki Page]])
[[EchoBASE]]
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[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1DW9 PDB:1DW9]
EchoBASE:EB0172
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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==References==
==References==
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{{BacktoTop}}
 
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<references/>
<references />
__NOTOC__
__NOTOC__
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===Categories===
===Categories===
{{BacktoTop}}
{{BacktoTop}}
[[Category:Proteins]]
[[Category:Proteins]]
[[Category:GO:0005737_!_cytoplasm]][[Category:GO:0009440_!_cyanate catabolic process]][[Category:GO:0006807_!_nitrogen compound metabolic process]][[Category:GO:0006805_!_xenobiotic metabolic process]][[Category:GO:0008824_!_cyanate hydratase activity]][[Category:Complex:cyanase]][[Category:Products with structures in PDB]]

Latest revision as of 20:24, 17 February 2015

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

CynS

Synonyms

cyanate aminohydrolase[1], B0340[2][1], Cnt[2][1], CyaN[2][1], CynS[2][1], cyanate hydrolase[2][1], cyanase[2][1], cyanate C-N-lyase[2][1] , cnt, ECK0337, JW0331, b0340

Product description

cyanase[2][3];

Component of cyanase[2]; cyanase dimer[3]; cyanase[3]

Cyanate aminohydrolase[4]

EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0005737

cytoplasm

PMID:8083164[5]

IDA: Inferred from Direct Assay

C

Seeded from Riley et al 2006 [1].

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010982

F

Seeded from EcoCyc (v14.0)

complete

GO:0008824

cyanate hydratase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00535

F

Seeded from EcoCyc (v14.0)

complete

GO:0008824

cyanate hydratase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003712

F

Seeded from EcoCyc (v14.0)

complete

GO:0008824

cyanate hydratase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:4.2.1.104

F

Seeded from EcoCyc (v14.0)

complete

GO:0016836

hydro-lyase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008076

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of cyanase

could be indirect

Protein

prs

PMID:16606699[6]

Experiment(s):EBI-1136296

Protein

dnaK

PMID:16606699[6]

Experiment(s):EBI-1136296

Protein

gshB

PMID:16606699[6]

Experiment(s):EBI-1136296
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MIQSQINRNI RLDLADAILL SKAKKDLSFA EIADGTGLAE AFVTAALLGQ QALPADAARL
VGAKLDLDED SILLLQMIPL RGCIDDRIPT DPTMYRFYEM LQVYGTTLKA LVHEKFGDGI
ISAINFKLDV KKVADPEGGE RAVITLDGKY LPTKPF
Length

156

Mol. Wt

17.049 kDa

pI

4.8 (calculated)

Extinction coefficient

5,960 - 6,085 (calc based on 4 Y, 0 W, and 1 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

83..156

PF02560 Cyanate lyase C-terminal domain

PMID:19920124[7]

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=cynS taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128325

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948998

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001173

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P00816

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10175

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10175

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948998

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000170

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0172

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ Kozliak, EI et al. (1994) Expression of proteins encoded by the Escherichia coli cyn operon: carbon dioxide-enhanced degradation of carbonic anhydrase. J. Bacteriol. 176 5711-7 PubMed
  6. ↑ 6.0 6.1 6.2 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  7. ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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