ynfE:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

YnfE

Synonyms

oxidoreductase subunit[1], B1587[2][1], YnfE[2][1] , ECK1582, JW1579, b1587

Product description

oxidoreductase subunit paralog of DmsA[2][3];

Component of dimethyl sulfoxide reductase paralogue[3]

Putative periplasmic reductase, function unknown[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0005506

iron ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0408

F

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-1003

C

Seeded from EcoCyc (v14.0)

complete

GO:0009055

electron carrier activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006655

F

Seeded from EcoCyc (v14.0)

complete

GO:0009389

dimethyl sulfoxide reductase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011888

F

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0472

C

Seeded from EcoCyc (v14.0)

complete

GO:0030151

molybdenum ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006657

F

Seeded from EcoCyc (v14.0)

complete

GO:0030151

molybdenum ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011888

F

Seeded from EcoCyc (v14.0)

complete

GO:0030151

molybdenum ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0500

F

Seeded from EcoCyc (v14.0)

complete

GO:0046872

metal ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0479

F

Seeded from EcoCyc (v14.0)

complete

GO:0051536

iron-sulfur cluster binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0411

F

Seeded from EcoCyc (v14.0)

complete

GO:0051539

4 iron, 4 sulfur cluster binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011888

F

Seeded from EcoCyc (v14.0)

complete

GO:0051539

4 iron, 4 sulfur cluster binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0004

F

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011888

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

P

Seeded from EcoCyc (v14.0)

complete

GO:0033797

selenate reductase activity

PMID:19415239[5]

IMP: Inferred from Mutant Phenotype

F

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of dimethyl sulfoxide reductase paralogue

could be indirect

Protein

dnaE

PMID:16606699[6]

Experiment(s):EBI-1140220

Protein

yfiD

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

glf

PMID:19402753[7]

LCMS(ID Probability):99.4

Protein

deaD

PMID:19402753[7]

LCMS(ID Probability):99.6

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MSKNERMVGI SRRTLVKSTA IGSLALAAGG FSLPFTLRNA AAAVQQAREK VVWGACSVNC
GSRCALRLHV KDNEVTWVET DNTGSDEYGN HQVRACLRGR SIRRRINHPD RLNYPMKRVG
KRGEGKFERI SWDEALDTIA SSLKKTVEQY GNEAVYIQYS SGIVGGNMTR SSPSASAVKR
LMNCYGGSLN QYGSYSTAQI SCAMPYTYGS NDGNSTTDIE NSKLVVMFGN NPAETRMSGG
GITYLLEKAR EKSNAKMIVI DPRYTDTAAG REDEWLPIRP GTDAALVAGI AWVLINENLV
DQPFLDKYCV GYDEKTLPAD APKNGHYKAY ILGEGDDKTA KTPQWASQIT GIPEDRIIKL
AREIGTAKPA YICQGWGPQR QANGELTARA IAMLPILTGN VGISGGNSGA RESTYTITIE
RLPVLDNPVK TSISCFSWTD AIDHGPQMTA IRDGVRGKDK LDVPIKFIWN YAGNTLVNQH
SDINKTHEIL QDESKCEMIV VIENFMTSSA KYADILLPDL MTVEQEDIIP NDYAGNMGYL
IFLQPVTSEK FERKPIYWIL SEVAKRLGPD VYQKFTEGRT QEQWLQHLYA KMLAKDPALP
SYDELKKMGI YKRKDPNGHF VAYKAFRDDP EANPLKTPSG KIEIYSSRLA EIARTWELEK
DEVISPLPVY ASTFEGWNSP ERRTFPLQLF GFHYKSRTHS TYGNIDLLKA ACRQEVWINP
IDAQKRGIAN GDMVRVFNHR GEVRLPAKVT PRILPGVSAM GQGAWHEANM SGDKIDHGGC
VNTLTTLRPS PLAKGNPQHT NLVEIEKI
Length

808

Mol. Wt

89.781 kDa

pI

8.5 (calculated)

Extinction coefficient

130,180 - 131,680 (calc based on 32 Y, 15 W, and 12 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

motif

1-43

UniProt Manual:Signal Peptides

UniProt:P77374

Domain

49..108

PF04879 Molybdopterin oxidoreductase Fe4S4 domain

PMID:19920124[8]

Domain

111..566

PF00384 Molybdopterin oxidoreductase

PMID:19920124[8]

Domain

687..802

PF01568 Molydopterin dinucleotide binding domain

PMID:19920124[8]

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=ynfE taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16129545

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946135

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0005302

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P77374

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:G6845

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG13843

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946135

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003434

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3604

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. Guymer, D et al. (2009) A genetic analysis of in vivo selenate reduction by Salmonella enterica serovar Typhimurium LT2 and Escherichia coli K12. Arch. Microbiol. 191 519-28 PubMed
  6. Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  7. 7.0 7.1 7.2 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  8. 8.0 8.1 8.2 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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