lhgO:Gene Product(s)

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

LhgO

Synonyms

predicted enzyme[1], B2660[2][1], YgaF[2][1] , ECK2654, JW2635, ygaF, b2660

Product description

predicted enzyme[2][3]

Putative dehydrogenase, function unknown; second gene in csiD-lhgO-gabDTP-csiR operon[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0034419

L-2-hydroxyglutarate oxidase activity

PMID:18390652[5]

IDA: Inferred from Direct Assay

F

Figures 4 and 5

complete

GO:0005737

cytoplasm

PMID:18390652[5]

IDA: Inferred from Direct Assay

C

complete

GO:0050660

FAD binding

PMID:18390652[5]

IDA: Inferred from Direct Assay

F

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

groL

PMID:16606699[6]

Experiment(s):EBI-1143593
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MAISLTPLTT TRRISKRKGI ERMYDFVIIG GGIIGMSTAM QLIDVYPDAR IALLEKESAP
ACHQTGHNSG VIHAGVYYTP GSLKAQFCLA GNRATKAFCD QNGIRYDNCG KMLVATSDLE
MERMRALWER TAANGIEREW LNADELRERE PNITGLGGIF VPSSGIVSYR DVTAAMAKIF
QSRGGEIIYN AEVSGLNEHK NGVVIRTRQG GEYEASTLIS CSGLMADRLV KMLGLEPGFI
ICPFRGEYFR LAPEHNQIVN HLIYPIPDPA MPFLGVHLTR MIDGSVTVGP NAVLAFKREG
YRKRDFSFSD TLEILGSSGI RRVLQNHLRS GLGEMKNSLC KSGYLRLVQK YCPRLSLSDL
QPWPAGVRAQ AVSPDGKLID DFLFVTTPRT IHTCNAPSPA ATSAIPIGAH IVSKVQTLLA
SQSNPGRTLR AARSVDALHA AFNQ
Length

444

Mol. Wt

48.593 kDa

pI

9.2 (calculated)

Extinction coefficient

35,870 - 36,995 (calc based on 13 Y, 3 W, and 9 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

3..392

PF01266 FAD dependent oxidoreductase

PMID:19920124[7]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=ygaF taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

Purification protocol

PMID:18390652[5]

Assay

PMID:18390652[5]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:90111477

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948069

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P37339

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948069

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 5.3 5.4 Kalliri, E et al. (2008) Identification of Escherichia coli YgaF as an L-2-hydroxyglutarate oxidase. J. Bacteriol. 190 3793-8 PubMed
  6. ↑ Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  7. ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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