ydhZ:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

FumD

Synonyms

FumD, B1675[1][2], YdhZ[1][2] , ECK1671, JW1665

Product description

In vitro catalyzes the addition of water to fumarate, forming malate. Cannot catalyze the reverse reaction. [3]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0004333

fumarate hydratase activity

PMID:27914785[4]

IDA: Inferred from Direct Assay

F

Fig. 3e, assayed with purified protein

complete

GO:0005829

cytosol

PMID:19054114[5]

IEA: Inferred from Electronic Annotation

PMID:19054114[5]


C

from EcoCyc ver. 21.0

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

udk

PMID:16606699[6]

Experiment(s):EBI-1140446

Protein

arcA

PMID:16606699[6]

Experiment(s):EBI-1140446

Protein

nadE

PMID:16606699[6]

Experiment(s):EBI-1140446

Protein

htpG

PMID:16606699[6]

Experiment(s):EBI-1140446

Protein

rplO

PMID:16606699[6]

Experiment(s):EBI-1140446

Protein

rplE

PMID:16606699[6]

Experiment(s):EBI-1140446

Protein

rpsI

PMID:16606699[6]

Experiment(s):EBI-1140446

Protein

rplF

PMID:16606699[6]

Experiment(s):EBI-1140446

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MGNRTKEDEL YREMCRVVGK VVLEMRDLGQ EPKHIVIAGV LRTALANKRI QRSELEKQAM
ETVINALVK
Length

69

Mol. Wt

7.906 kDa

pI

9.7 (calculated)

Extinction coefficient

1,490 - 1,615 (calc based on 1 Y, 0 W, and 1 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

1..69

PF10965 Protein of unknown function (DUF2767)

PMID:19920124[7]

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=ydhZ taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16129631

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946180

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0005591

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0ACX5

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:G6903

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG13960

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946180

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003492

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3718

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  2. 2.0 2.1 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  3. Sévin, DC et al. (2017) Nontargeted in vitro metabolomics for high-throughput identification of novel enzymes in Escherichia coli. Nat. Methods 14 187-194 PubMed
  4. Chen, W et al. (2017) Effect of macroporous adsorption resin-membrane bioreactor hybrid system against fouling for municipal wastewater treatment. Bioresour. Technol. 224 112-117 PubMed
  5. 5.0 5.1 Díaz-Mejía, JJ et al. (2009) Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome. FEMS Microbiol. Rev. 33 66-97 PubMed
  6. 6.0 6.1 6.2 6.3 6.4 6.5 6.6 6.7 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  7. Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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