wzxE:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

WzxE

Synonyms

O-antigen translocase[1], B3792[2][1], Wzx[2][1], YifJ[2][1], WzxE[2][1] , ECK3784, JW3766, wzx, yifJ, b3792

Product description

lipid III flippase[2][3];

Component of Enterobacterial Common Antigen Biosynthesis Protein Complex[3]

O-antigen translocase; involved in the cross-membrane translocation of the UDP-linked ECA trisaccharide repeat unit of cyclic enterobacterial common antigen ECA(CYC)[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0000271

polysaccharide biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002797

P

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0997

C

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-1003

C

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002797

C

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0472

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0812

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-9909

C

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of Enterobacterial Common Antigen Biosynthesis Protein Complex

could be indirect


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

plasma membrane

C-terminus localized in the cytoplasm with 12 predicted transmembrane domains

Daley et al. (2005) [5]

plasma membrane

From EcoCyc[3]


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MSLAKASLWT AASTLVKIGA GLLVGKLLAV SFGPAGLGLA ANFRQLITVL GVLAGAGIFN
GVTKYVAQYH DNPQQLRRVV GTSSAMVLGF STLMALVFVL AAAPISQGLF GNTDYQGLVR
LVALVQMGIA WGNLLLALMK GFRDAAGNAL SLIVGSLIGV LAYYVSYRLG GYEGALLGLA
LIPALVVIPA AIMLIKRGVI PLSYLKPSWD NGLAGQLSKF TLMALITSVT LPVAYIMMRK
LLAAQYSWDE VGIWQGVSSI SDAYLQFITA SFSVYLLPTL SRLTEKRDIT REVVKSLKFV
LPAVAAASFT VWLLRDFAIW LLLSNKFTAM RDLFAWQLVG DVLKVGAYVF GYLVIAKASL
RFYILAEVSQ FTLLMVFAHW LIPAHGALGA AQAYMATYIV YFSLCCGVFL LWRRRA
Length

416

Mol. Wt

44.961 kDa

pI

10.3 (calculated)

Extinction coefficient

81,820 - 82,070 (calc based on 18 Y, 10 W, and 2 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

3..284

PF01943 Polysaccharide biosynthesis protein

PMID:19920124[6]

<motif_map/>

tmhmm.php?gene=wzxE&show=pic&.jpg
Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=wzxE taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131648

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948294

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012386

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0AAA7

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG11486

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11486

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948294

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001442

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1449

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
  6. Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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